Note: Front Cover -- Bioluminescence and Chemiluminescence -- Copyright Page -- Table of Contents -- Contributors to Volume 305 -- Preface -- Volumes in Series -- Section I: Chemiluminescence and Bioluminescence: Overviews -- Chapter 1. Chemical Generation of Excited States: The Basis of Chemiluminescence and Bioluminescence -- Chapter 2. Some Brief Notes on Nomenclature and Units and Standards Used in Bioluminescence and Chemiluminescence -- Section II: Instrumentation -- Chapter 3. Physics of Low Light Detectors -- Chapter 4. Luminometer Design and Low Light Detection -- Chapter 5. Absolute Calibration of Luminometers with Low-Level Light Standards -- Chapter 6. Commercially Available Luminometers and Low-Level Light Imaging Devices -- Chapter 7. Automated Recordings of Bioluminescence with Special Reference to the Analysis of Circadian Rhythms -- Chapter 8. Chemiluminescence Imaging Systems for the Analysis of Macrosamples: Microtiter Format, Blot Membrane, and Whole Organs -- Section III: Luciferases, Luminescence Accessory Proteins, and Substrates -- Chapter 9. Overexpression of Bacterial Luciferase and Purification from Recombinant Sources -- Chapter 10. Purification of Luciferase Subunits from Recombinant Sources -- Chapter 11. A Rapid Chromatographic Method to Separate the Subunits of Bacterial Luciferase in Urea Containing Buffer -- Chapter 12. Purification and Ligand Exchange Protocols for Antenna Proteins from Bioluminescent Bacteria -- Chapter 13. Purification of Firefly Luciferase from Recombinant Sources -- Chapter 14. Chemical Synthesis of Firefly Luciferin Analogs and Inhibitors -- Chapter 15. Structural Basis for Understanding Spectral Variations in Green Fluorescent Protein -- Chapter 16. Large-Scale Purification of Recombinant GFP from Escherichia coli. , Chapter 17. Recombinant Obelin: Cloning and Expression of cDNA, Purification, and Characterization as a Calcium Indicator -- Chapter 18. Gonyaulax Luciferase: Gene Structure, Protein Expression, and Purification from Recombinant Sources -- Chapter 19. Dinoflagellate Luciferin-Binding Protein -- Section IV: Bacterial Autoinduction System and Its Applications -- Chapter 20. Assay of Autoinducer Activity with Luminescent Escherichia coli Sensor Strains Harboring a Modified Vibrio fischeri lux Regulon -- Chapter 21. Detection, Purification, and Structural Elucidation of the Acylhomoserine Lactone Inducer of Vibrio fischeri Luminescence and Other Related Molecules -- Chapter 22. Chemical Synthesis of Bacterial Autoinducers and Analogs -- Chapter 23. Overexpression of Foreign Proteins Using the Vibrio fischeri lux Control System -- Section V: Luminescence-Based Assays in Vitro -- Chapter 24. Application of Bioluminescence and Chemiluminescence in the Biomedical Sciences -- Chapter 25. Use of Firefly Luciferase in ATP-Related Assays of Biomass, Enzymes, and Metabolites -- Chapter 26. Chemiluminescent Methods for Detecting and Quantitating Enzyme Activity -- Chapter 27. Chemiluminescence Assay of Serum Alkaline Phosphatase and Phosphoprotein Phosphatases -- Chapter 28. Chemiluminescence Screening Assays for Erythrocytes and Leukocytes in Urine -- Chapter 29. Immunoassay Protocol for Quantitation of Protein Kinase Activities -- Chapter 30. Chemiluminescent Immunodetection Protocols with 1,2-Dioxetane Substrates -- Chapter 31. Chemiluminescent Reporter Gene Assays with 1,2-Dioxetane Substrates -- Chapter 32. Clinical Application of Southern Blot Hybridization with Chemiluminescence Detection -- Chapter 33. Quantitative Polymerase Chain Reaction and Solid-Phase Capture Nucleic Acid Detection -- Section VI: Luminescence Monitoring in Vivo. , Chapter 34. Targeted Bioluminescent Indicators in Living Cells -- Chapter 35. Green Fluorescent Protein as a Reporter of Transcriptional Activity in a Prokaryotic System -- Chapter 36. Bacterial lux Genes as Reporters in Cyanobacteria -- Chapter 37. Application of Bioluminescence to the Study of Circadian Rhythms in Cyanobacteria -- Chapter 38. Construction of lux Bacteriophages and the Determination of Specific Bacteria and Their Antibiotic Sensitivities -- Chapter 39. Luciferase Gene as Reporter: Comparison with the CAT Gene and Use in Transfection and Microinjection of Mammalian Cells -- Chapter 40. In Situ Hybridization and Immunohistochemistry with Enzyme-Triggered Chemiluminescent Probes -- Chapter 41. Blood Phagocyte Luminescence: Gauging Systemic Immune Activation -- Section VII: Bioluminescence as an Education Tool -- Chapter 42. Demonstrations of Chemiluminescence -- Chapter 43. Bioluminescence as a Classroom Tool for Scientist Volunteers -- Chapter 44. Green Fluorescent Protein in Biotechnology Education -- Author Index -- Subject Index.