Publication Date:
2022-05-25
Description:
Author Posting. © Society for General Mircobiology, 2002. This article is posted here by permission of Society for General Mircobiology for personal use, not for redistribution. The definitive version was published in International Journal of Systematic Bacteriology 52 (2002): 2261-2269, doi:10.1099/ijs.0.02184-0.
Description:
A cellulolytic, dinitrogen-fixing bacterium isolated from the gill tissue of a
wood-boring mollusc (shipworm) Lyrodus pedicellatus of the bivalve family
Teredinidae and 58 additional strains with similar properties, isolated from
gills of 24 bivalve species representing 9 of 14 genera of Teredinidae, are
described. The cells are Gram-negative, rigid, rods (0〈4–0〈6x3–6 lm) that bear a
single polar flagellum. All isolates are capable of chemoheterotrophic growth
in a simple mineral medium supplemented with cellulose as a sole source of
carbon and energy. Xylan, pectin, carboxymethylcellulose, cellobiose and a
variety of sugars and organic acids also support growth. Growth requires
addition of combined nitrogen when cultures are vigorously aerated, but all
isolates fix dinitrogen under microaerobic conditions. The pH, temperature and
salinity optima for growth were determined for six isolates and are
approximately 8〈5, 30–35 °C and 0〈3 M NaCl respectively. The isolates are
marine. In addition to NaCl, growth requires elevated concentrations of Ca2M
and Mg2M that reflect the chemistry of seawater. The DNA GMC content ranged
from 49 to 51 mol%. Four isolates were identical with respect to small-subunit
rRNA sequence over 891 positions compared and fall within a unique clade in
the c-subclass of the Proteobacteria. Based on morphological, physiological
and phylogenetic characteristics and specific symbiotic association with
teredinid bivalves, a new genus and species, Teredinibacter turnerae gen. nov.,
sp. nov., is proposed. The type strain is T7902T (vATCC 39867TvDSM 15152T).
Description:
This work was supported by grants from the National
Science Foundation no. NSF DEB-9420051 and IBN-
9982982, the Maine Science and Technology Foundation's
Center for Innovation in Biotechnology, and the University
of Maine's Faculty Research program.
Keywords:
Teredinidae
;
Bivalvia
;
Shipworms
;
Symbiont
;
16S rRNA phylogeny
Repository Name:
Woods Hole Open Access Server
Type:
Article
Format:
411866 bytes
Format:
application/pdf
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