ISSN:
1617-4623
Keywords:
Bacteriophage T7
;
Monomer replication
;
Concatemer replication
;
Primer RNA
;
Semidiscontinous replication
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have analyzed the transition sites between primer RNA and DNA in a 589 bp segment of the bacteriophage T7 genome. In the monomeric replication stage, RNA-DNA transition sites are predominantly on the light (L) strand (with, 5′→3′ polarity on the genetic map) but rarely on the heavy (H) strand, indicating that replication proceeds semidiscontinuously with the H and L strands corresponding to the leading and lagging strands, respectively. The direction of replication is that expected from the position of the primary origin and also indicates that secondary origins are seldom if ever used. In the concatemeric stage of replication, RNA-DNA transition sites are instead distributed on both strands of the segment with equally high frequency, showing that initiation occurs within the concatemeric molecule per se and by a different mechanism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425692
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