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  • Bacillus thuringiensis δ-endotoxin gene  (1)
  • RNA synthesis  (1)
Document type
Keywords
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Years
  • 1
    Electronic Resource
    Electronic Resource
    Transcription during early embryogenesis ofLeptinotarsa (Coleoptera) (1979)
    Springer
    Development genes and evolution 186 (1979), S. 179-188 
    Details
    ISSN: 1432-041X
    Keywords: Insects ; Early embryogenesis ; RNA synthesis ; α-amanitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RNA synthesis was investigated in precleavage stages (0–5 h after egg deposition) and cleavage stages (5–36 h) ofLeptinotarsa by autoradiography. In precleavage stages the first detectable RNA synthesis occurs after the maturation divisions. Label is found in the female pronucleus during the short period of migration towards the interior of the egg and in the polar body nuclei. In no case was label found in the sperm pronuclei or during the period when the female pronucleus is attached to a male pronucleus prior to fusion (pronuclear prefusion phase). During cleavage, intranuclear labelling can be shown at all developmental stages except the 2-nuclei stage. RNA synthesis increases with age. α-Amanitin completely inhibits the autoradiographically detectable RNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848588
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Development of insect resistance in tomato plants expressing the δ-endotoxin gene ofBacillus thuringiensis subsp.tenebrionis (1995)
    Springer
    Molecular breeding 1 (1995), S. 229-236 
    Details
    ISSN: 1572-9788
    Keywords: Bacillus thuringiensis δ-endotoxin gene ; Coleoptera ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A crystal δ-endotoxin gene ofBacillus thuringiensis subsp.tenebrionis (B.t.t.) encoding a coleopteran insect-specific toxin was used to construct a chimeric gene which expressed the toxin in plant cells. Via anAgrobacterium tumefaciens binary vector system, the toxin gene was transferred into tomato cells. From leaf disks recombinant plants were regenerated. Hybridization experiments demonstrated that these plants synthesized toxin-specific mRNA of the expected size. Transgenic tomato plants with the chimericB.t.t. toxin gene contained a 74 kDa protein which cross-reacted with toxin antibodies. The expression caused a significant insecticidal activity of the transgenic tomato plants against Colorado potato beetle larvae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02277423
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    Paper (German National Licenses)
    Fulltext
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