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  • Autologous mixed lymphocyte reaction  (2)
  • Bcl-2  (2)
  • autologous mixed lymphocyte reaction  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Autologous mixed lymphocyte reaction in man. II. Histamine-induced suppression of the autologous mixed lymphocyte reaction by T-cell subsets defined with monoclonal antibodies (1981)
    Springer
    Journal of clinical immunology 1 (1981), S. 241-249 
    Details
    ISSN: 1573-2592
    Keywords: T-cell subsets ; histamine-induced suppressor function ; autologous mixed lymphocyte reaction ; monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Human peripheral blood mononuclear cells were separted into T and non-T cells by E-rosette formation. The influence of histamine (10−8−10−3 M) on the proliferative response of T cells in autologous and allogeneic mixed lymphocyte cultures (MLC) was studied. Pretreatment of responder T cells but not of stimulator non-T cells with histamine for 24 hr resulted in a markedly diminished proliferative response in both autologous and allogeneic MLC. A minimum of 4 hr of incubation of T cells with histamine was required to inhibit autologous MLC. Furthermore, T cells pretreated with histamine followed by mitomycin C treatment, when cocultured with fresh autologous T cells, suppressed their proliferative response in both autologous and allogeneic MLC. Analysis with OKT4 and OKT8 monoclonal antibodies revealed that histamine-induced suppressor T cells were contained in OKT 8 + -cell subset. Hitamine-treated OKT 4 + cells had no suppressive effect on the proliferative responses of autologous T cells. Supernatants of T cells cultured with histamine for 24 hr also suppressed both autologous and allogeneic MLC responses of fresh T cells, suggesting that the suppression could be mediated by a soluble suppressor factor(s). Experiments with H1 and H2 agonists and antagonists indicated that histamine-induced activation of suppressor T cells and the production of a soluble suppressor factor(s) were mediated through histamine type 2 receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00915143
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  • 2
    Electronic Resource
    Electronic Resource
    Lymphocyte transformation induced by autologous cells. XV. Xenoantigens are not required for the proliferative response observed in the autologous mixed lymphocyte reaction (1983)
    Springer
    Journal of clinical immunology 3 (1983), S. 100-102 
    Details
    ISSN: 1573-2592
    Keywords: Autologous mixed lymphocyte reaction ; sheep erythrocytes ; fetal calf serum ; xenoantigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The autologous mixed lymphocyte reaction is the proliferative response of T cells cultured with autologous non-T cells. This reaction has been described as being immunological in nature, i.e., possessing memory and specificity. The generation of T cells with regulatory and effector function has also been reported during the course of this reaction. More recently it has been reported that the proliferative response observed is due to the exposure of T cells to xenoantigens used in separating T and non-T cells. We have found that the presence of antigens such as sheep erythrocytes and fetal calf serum is not required for the induction of proliferation in T cells by autologous non-T cells, although exposure to such antigens may augment [3H]thymidine incorporation. In certain individuals who are sensitized to these xenogeneic antigens, the proliferative response of their lymphocytes in the autologous mixed lymphocyte reaction is very greatly enhanced if exposed to xenoantigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00919145
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Programmed Cell Death (Apoptosis) in Cord Blood Lymphocytes (1997)
    Springer
    Journal of clinical immunology 17 (1997), S. 63-73 
    Details
    ISSN: 1573-2592
    Keywords: Cord blood ; apoptosis ; Fas ; Bcl-2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cord blood lymphocytes are functionally immature and have deficient immune responses. In order to determine whether the process of programmed cell death is distinct between cord blood and peripheral blood lymphocytes, we analyzed the expression of fas and bax (apoptosis promoting genes) and bcl-2 and bcl-x L (apoptosis inhibiting genes) at protein or mRNA levels using flow cytometry and quantitative PCR methods, respectively. The susceptibility of T cell subsets from cord blood and adult peripheral blood to undergo apoptosis following restimulation with anti-CD3 or anti-Fas monoclonal antibodies was also studied. We observed that cord blood T cell subsets expressed lower levels of Fas and Bcl-2, a low bcl-2/bax ratio, and higher bcl-x L compared to peripheral blood. Additionally, upon primary stimulation with anti-CD3, cord blood T cell subsets were more resistant to apoptosis compared to peripheral blood. In contrast, rechallenge of previously stimulated lymphocytes with anti-CD3 monoclonal antibody triggered apoptosis in a larger proportion of T cells from cord blood as compared to peripheral blood, whereas the number of T cells undergoing anti-Fas-induced programmed cell death were lower in cord blood compared to peripheral blood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1027340529644
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  • 4
    Electronic Resource
    Electronic Resource
    Cartilage-Hair Hypoplasia Syndrome: Increased Apoptosis of T Lymphocytes Is Associated with Altered Expression of Fas (CD95), FasL (CD95L), IAP, Bax, and Bcl2 (1999)
    Springer
    Journal of clinical immunology 19 (1999), S. 428-434 
    Details
    ISSN: 1573-2592
    Keywords: Cartilage-hair hypoplasia ; T cell subsets ; cytokines ; Fas ; FasL ; Bcl-2 ; Bax
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cartilage-hair hypoplasia (CHH) is a rare autosomal recessive short-limbed dwarfism associated with thin and sparse hair and cell mediated or combined immunodeficiency. However, the basis of immune deficiency in CHH is unclear. In this study, we investigated a role of apoptosis in immunodeficiency in a patient with CHH. An increased apoptosis of both CD4+ and CD8+ T cells, as determined by TUNEL assay, was observed in CHH compared to an age-matched healthy dwarf control. Increased apoptosis in CHH was associated with increased expression of Fas (CD95), CD95L, and Bax and decreased expression of Bcl-2 and inhibitor of apoptosis protein (IAP) compared to the control. These data suggest that lymphopenia and immunodeficiency in CHH may be, at least in part, due to increased apoptosis of T cells, possibly through the Fas/ FasL signaling pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020563018864
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  • 5
    Electronic Resource
    Electronic Resource
    Autologous mixed lymphocyte reaction in man. IV. Decreased autologous mixed lymphocyte culture response in patients with common variable immunodeficiency (1983)
    Springer
    Journal of clinical immunology 3 (1983), S. 78-83 
    Details
    ISSN: 1573-2592
    Keywords: Autologous mixed lymphocyte reaction ; primary immunodeficiency ; T-cell subsets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The autologous mixed lymphocyte reaction (AMLR) was studied in 18 patients with common variable immunodeficiency. The AMLR was decreased in 10 of 18 (55%) patients with common variable immunodeficiency compared to healthy controls. In allogeneic MLR, T cells from patients were found to be poor responders, and non-T cells poor stimulators, compared to allogeneic MLR between healthy normal controls. In allogeneic MLR, B cells (B cells + null cells) from patients were poor stimulators, whereas macrophages stimulated normally compared to controls. The deficient AMLR could be one of the mechanisms responsible for the increased risk of autoimmune phenomena in a subset of patients with primary immunodeficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00919142
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  • 6
    Electronic Resource
    Electronic Resource
    Autologous mixed lymphocyte reaction in man. XIII. Characterization of the T-T autologous mixed lymphocyte reaction (1985)
    Springer
    Journal of clinical immunology 5 (1985), S. 187-194 
    Details
    ISSN: 1573-2592
    Keywords: T-cell subsets ; autologous mixed lymphocyte reaction ; interleukin-2 (IL-2) ; IL-2 receptor ; suppressor function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In this study we have demonstrated that in the T-TA autologous mixed lymphocyte reaction (AMLR), OKT4+ T cells are the major responders; however, in the presence of additional interleukin-2 (IL-2), OKT8+ T cells also respond by proliferation. Both OKT4+ and OKT8+ T cells, activated in the T-non-T AMLR, act as stimulators in the T-TA AMLR. OKT4+ T cells activated in the T-TA AMLR suppress the proliferative response of the fresh T-non-T AMLR; control OKT4+ cells show no immunoregulatory activity in this system. In contrast, control OKT8+ T cells spontaneously suppress the proliferation of the T-non-T AMLR, but activation of OKT8+ T cells in the T-TA AMLR does not result in a further increase in the suppressor activity of OKT8+ T cells. In summary, in the T-non-T and T-TA AMLR phenotypically similar T-cell subpopulations proliferate but express distinct immunoregulatory functions and perhaps regulate the tempo of the AMLR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00915510
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