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  • Archaea; Baltic Sea; BaltRap; BUCKET; Bucket water sampling; CARD-FISH; Catalyzed reporter deposition fluorescence in situ hybridisation (CARD-FISH); Cells, 4',6-Diamidin-2-phenylindol stained; Core acyclic glycerol dialkyl glycerol tetraether; Core crenarchaeol; Core crenarchaeol regio-isomer; Core dicyclic glycerol dialkyl glycerol tetraether; core lipids; Core monocyclic glycerol dialkyl glycerol tetraether; Core tricyclic glycerol dialkyl glycerol tetraether; Crenarchaeota, targeted with Cren537 oligonucleotide FISH-probe; CTD, Sea-Bird, SBE 911plus; CTD/Rosette; CTD-RO; DATE/TIME; DEPTH, water; Dihexose acyclic glycerol dialkyl glycerol tetraether; Dihexose-archaeol; Dihexose crenarchaeol; Dihexose dicyclic glycerol dialkyl glycerol tetraether; Dihexose monocyclic glycerol dialkyl glycerol tetraether; Dihexose tricyclic glycerol dialkyl glycerol tetraether; Eastern Gotland Basin; Elisabeth Mann Borgese; EMB201; EMB201_10-3; EMB201_10-9; EMB201_12-0; EMB201_12-4; EMB201_12-5; EMB201_5-3; EMB201_7-0; EMB201_7-1; EMB201_9-0; EMB201_9-1; Event label; Fårö Basin; glycerol dialkyl glycerol tetraethers; GOFLO; Go-Flo bottles; GPUMP; High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC; Intact polar lipids; Landsort Deep; LATITUDE; LC-MS/MS; Location; LONGITUDE; Monohexose acyclic glycerol dialkyl glycerol tetraether; Monohexose-archaeol; Monohexose crenarchaeol; Monohexose crenarchaeol regio-isomer; Monohexose dicyclic glycerol dialkyl glycerol tetraether; Monohexose-macroarchaeol; Monohexose monocyclic glycerol dialkyl glycerol tetraether; Monohexose tricyclic glycerol dialkyl glycerol tetraether; Other event; Oxygen, dissolved; Phosphohexose acyclic glycerol dialkyl glycerol tetraether; Phosphohexose crenarchaeol; Phosphohexose dicyclic glycerol dialkyl glycerol tetraether; Phosphohexose monocyclic glycerol dialkyl glycerol tetraether; Phosphohexose tricyclic glycerol dialkyl glycerol tetraether; Sample code/label; Ships non-toxic pump; Site; Stat. 06; Stat. TF 271 Stat. 4; Station 04 (TF 271); Station 09; Station label; Temperature, water; Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus  (1)
  • Intracellular oxygen transport  (1)
  • Klimaänderung  (1)
Document type
Keywords
Publisher
Language
Years
  • 1
    Online Resource
    Online Resource
    The impact of climate change on phytoplankton - bacterioplankton interactions (2009)
    Details Availability
    Keywords: Hochschulschrift ; Phytoplankton ; Klimaänderung
    Description / Table of Contents: Global warming has already and is continuing to impact the global oceans. Half of the global primary production is performed by phytoplankton in the oceans and heterotrophic marine bacteria channel a substantial amount of primary organic carbon through the microbial loop. Understanding the influence of climate change on these important processes is therefore essential for an assessment of the vulnerability of the carbon cycle and possible feedbacks. This thesis reports results from investigations on the temperature dependent coupling between phytoplankton and bacterioplankton, with respect to additional effects of light intensity and inorganic nutrient concentrations. During four consecutive years, mesocosm experiments with natural Kiel Fjord winter plankton communities investigated the influences of increasing water temperatures of up to ?T +6ʿC and different light intensities between 16 and 100% of natural incident light. In an additional microcosm experiment with a single algal species and the natural bacterial community, two inorganic nutrient concentrations were used, in order to evaluate the combined effects of temperature and substrate on the algal-bacterial coupling. Summarising the results from all experiments it can be concluded, that increasing temperatures generally led to an increased heterotrophic bacterial organic substrate utilisation relative to primary production. In combination with a further brightening, the supplemental promotion of primary production would increase the absolute amounts of cycled organic matter. Future increasing P-limitation in coastal waters would lead not only to an enhanced absolute amount of cycled carbon, but additionally to an increased relative amount of remineralised organic carbon through the microbial loop. An enhanced organic matter transfer through the microbial loop has the potential to alter the whole structure and functioning of the marine food web and the biological sequestration of carbon to depth. Additionally, a substantial rise of CO2 emissions through enhanced respiration represents a positive feedback loop to the global climate change problem.
    Type of Medium: Online Resource
    Pages: Online-Ressource (pdf-Datei: 199 S., 1,7 MB)
    URL: http://eldiss.uni-kiel.de/accept.htm?http://eldiss.uni-kiel.de/macau/servlets/MCRFileNodeServlet/dissertation_derivate_00003001/diss_pbreithaupt_2009.pdf?hosts=
    URL: http://nbn-resolving.de/urn:nbn:de:gbv:8-diss-44468
    URL: http://d-nb.info/1019869682/34
    URL: http://macau.uni-kiel.de/receive/dissertation_diss_00004446
    URN: urn:nbn:de:gbv:8-diss-44468
    DDC: 578.77622
    Language: English
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    GEOMAR CATALOGUE
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  • 2
    Electronic Resource
    Electronic Resource
    Diffusion of myoglobin in skeletal muscle cells — dependence on fibre type, contraction and temperature (1995)
    Springer
    Pflügers Archiv 430 (1995), S. 519-525 
    Details
    ISSN: 1432-2013
    Keywords: Diffusion coefficient ; Muscle cells ; Myoglobin ; Microinjection ; Oxygen ; Facilitated diffusion ; Intracellular oxygen transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We measured the diffusion coefficient of myoglobin (D Mb) inside mammalian skeletal muscle cells with a microinjection technique. A small bolus of horse Mb was injected into a single muscle fibre and the subsequent time-dependent changes of the Mb profiles along the fibre axis were measured with a microscope-photometer. For fibres of the rat soleus muscle at 22° C, a D Mb of 1.3·10−7 cm2/s was found, confirming a result obtained previously by us for rat diaphragm muscle with a photo-oxidation technique. In the extensor digitorum longus muscle of the rat, a higher value of 1.9 · 10−7 cm2/s was measured. Auxotonic muscle contractions did not change the apparent D Mb. For the temperature range between 22 ° C and 37 ° C, a temperature coefficient, Q 10, of 1.5 was calculated. The implication of this result for the role of Mb in the facilitation of oxygen transport was examined. Model calculations show that with this relatively low D Mb value, the intracellular oxygen supply can be improved only slightly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00373888
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Glycerol dialkyl glycerol tetraethers (GDGT) lipids and CARD-FISH data from suspended particulate matter from the central Baltic Sea (2023)
    PANGAEA
    Details
    Publication Date: 2024-05-30
    Description: During expedition EMB201 in the Baltic Sea we investigated the local producers of glycerol dialkyl glycerol tetraethers (GDGT) and their thriving depth by a combined 16S rRNA gene amplicon sequencing/ CARD-FISH and lipidomic approach. Water samples were taken in December 2018 by a pump-CTD, a giant water sampler and with Niskin bottles at the surface, suboxic and sulfidic zones of the Landsort Deep, Fårö Basin and East Gotland Basin. This data set contains the CARD-FISH and lipidomics data, while the 16S rRNA gene sequencing data is available on ENA. Cell abundance was analysed in an aliquot of 40 ml filtered (pore size 0.22 micro m) sea water fixed with particle-free formaldehyde. Archaeal cells on the filters were specifically hybridized via catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) using the Cren537 probe. Cells on the hybridized filter were counter-stained with 40,6-diamidin-2-phenylindol (DAPI). For lipid analysis, 150–600 L sea water were filtered with a flow rate of 1.5 L min-1 on pre-ashed, 142-mm-diameter, 0.7µm pore size glass fibre GF/F filters, and frozen at –20 °C. The filters were lyophilized before different extraction methods were used to obtain intact and core GDGTs by ultra-sonification in different solvent mixtures. The combined supernatants were phase separated before analysis by high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS2) for intact polar lipids and by high performance liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry (HPLC APCI-MS; ThermoScientific) for core GDGTs.
    Keywords: Archaea; Baltic Sea; BaltRap; BUCKET; Bucket water sampling; CARD-FISH; Catalyzed reporter deposition fluorescence in situ hybridisation (CARD-FISH); Cells, 4',6-Diamidin-2-phenylindol stained; Core acyclic glycerol dialkyl glycerol tetraether; Core crenarchaeol; Core crenarchaeol regio-isomer; Core dicyclic glycerol dialkyl glycerol tetraether; core lipids; Core monocyclic glycerol dialkyl glycerol tetraether; Core tricyclic glycerol dialkyl glycerol tetraether; Crenarchaeota, targeted with Cren537 oligonucleotide FISH-probe; CTD, Sea-Bird, SBE 911plus; CTD/Rosette; CTD-RO; DATE/TIME; DEPTH, water; Dihexose acyclic glycerol dialkyl glycerol tetraether; Dihexose-archaeol; Dihexose crenarchaeol; Dihexose dicyclic glycerol dialkyl glycerol tetraether; Dihexose monocyclic glycerol dialkyl glycerol tetraether; Dihexose tricyclic glycerol dialkyl glycerol tetraether; Eastern Gotland Basin; Elisabeth Mann Borgese; EMB201; EMB201_10-3; EMB201_10-9; EMB201_12-0; EMB201_12-4; EMB201_12-5; EMB201_5-3; EMB201_7-0; EMB201_7-1; EMB201_9-0; EMB201_9-1; Event label; Fårö Basin; glycerol dialkyl glycerol tetraethers; GOFLO; Go-Flo bottles; GPUMP; High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC; Intact polar lipids; Landsort Deep; LATITUDE; LC-MS/MS; Location; LONGITUDE; Monohexose acyclic glycerol dialkyl glycerol tetraether; Monohexose-archaeol; Monohexose crenarchaeol; Monohexose crenarchaeol regio-isomer; Monohexose dicyclic glycerol dialkyl glycerol tetraether; Monohexose-macroarchaeol; Monohexose monocyclic glycerol dialkyl glycerol tetraether; Monohexose tricyclic glycerol dialkyl glycerol tetraether; Other event; Oxygen, dissolved; Phosphohexose acyclic glycerol dialkyl glycerol tetraether; Phosphohexose crenarchaeol; Phosphohexose dicyclic glycerol dialkyl glycerol tetraether; Phosphohexose monocyclic glycerol dialkyl glycerol tetraether; Phosphohexose tricyclic glycerol dialkyl glycerol tetraether; Sample code/label; Ships non-toxic pump; Site; Stat. 06; Stat. TF 271 Stat. 4; Station 04 (TF 271); Station 09; Station label; Temperature, water; Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus
    Type: Dataset
    Format: text/tab-separated-values, 371 data points
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    DATA PANGAEA
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