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  • Arachis hypogaea  (2)
  • Life and Medical Sciences  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants (1996)
    Springer
    Plant molecular biology 30 (1996), S. 351-358 
    Details
    ISSN: 1573-5028
    Keywords: Arachis hypogaea ; Botrytis cinerea ; chitinase ; fungal elicitior ; ethylene ; salicylate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;1 and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea. The second gene, A.h.Chi2;1, was only expressed after treatment with the fungal spores. Transgenic tobacco plants containing the complete peanut A.h.Chi2;1 gene exhibited essentially the same expression pattern in leaves as observed in peanut cell cultures. Expression characteristics of transgenic tobacco carrying a promoter-GUS fusion of A.h.Chi2;1 are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020121
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Elicitor-specific induction of one member of the chitinase gene family in Arachis hypogaea (1990)
    Springer
    Molecular genetics and genomics 224 (1990), S. 469-476 
    Details
    ISSN: 1617-4623
    Keywords: Cell culture ; Phytophthora megasperma elicitor ; Signal transduction ; Chitinase cDNA ; Arachis hypogaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chitinases are believed to play an important role in plant defence against bacterial and fungal attack. In peanut (Arachis hypogaea) chitinase genes form a small multigene family. Four chitinase cDNAs (chit 1–4) were isolated from cultured peanut cells. Expression of individual chit genes was assayed by the polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphisms (RFLP). UV irradiation, dilution of cell cultures and treatment with Phytophthora megasperma (Pmg) elicitor or yeast extract were used to induce expression of chit genes. The chit 3 gene is constitutively expressed at a low level in untreated as well as in treated cultures; the expression of chit 4 gene is induced by each of the stimuli tested, whereas the chit 1 gene is activated by cell culture dilution and by yeast extract treatment. The chit 2 gene is strongly activated by treatment with cell wall components from the fungus Phytophthora megasperma but not by the other stimuli. These results indicate that chit 2 gene expression may be controlled by pathogen-specific regulatory elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262442
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Chimeric genes and transgenic plants are used to study the regulation of genes involved in symbiotic plant-microbe interactions (nodulin genes) (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 182-196 
    Details
    ISSN: 0192-253X
    Keywords: nodulins ; leghemoglobin ; glutamine synthetase ; Enod2 ; cis-acting elements ; transacting factors ; Agrobacterium turnefaciens ; A. rhizogenes ; binary vectors ; plant transformation ; chimeric genes ; chloramphenicol acetyltransferase ; glucuronidase ; cytokinin induction ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Nodulin genes are plant genes specifically activated during the formation of nitrogen-fixing nodules on leguminous plants. These genes are interesting to study since they are not only induced in a specific developmental fashion by signals coming directly or indirectly from the rhizobial symbiont, but are also expressed in a tissue-specific manner. By examining the expression of chimeric nodulin-reporter genes in transgenic legume plants it has been shown that nodule specific expression is mediated by DNA sequences present in the 5′upstream region of several nodulin genes. Here we summarize the available data on these cis-acting elements and the trans-acting factors interacting with them. We also review experiments designed to identify rhizobial “signals” which may play a role in nodule specific gene expression.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110304
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Bacterial and firefly luciferase genes in transgenic plants: Advantages and disadvantages of a reporter gene (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 224-232 
    Details
    ISSN: 0192-253X
    Keywords: lux ; luc reporter genes ; light emission ; gene expression ; single photon imaging in vivo ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Genes encoding light-emitting luciferase were recently isolated from luminous marine bacteria and fireflies. Expression of luciferase genes in diverse organisms is a unique way for studying gene expression by simple and sensitive measurement of light. Recent advances in application of luciferase reporter genes are reviewed and documented by examples of in vivo visualization of their expression in transgenic plants.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110308
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    Paper (German National Licenses)
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