ISSN:
1432-2277
Keywords:
Immunosuppression and metalloproteinases
;
Metalloproteinases and immunosuppression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The concentration of the metalloproteinases type I collagenase and gelatinase was measured in isolated polymorphonuclear leukocytes (PMNLs) of renal transplant recipients treated either with cyclosporin A (CyA) and prednisolone (Pr) (n=8) or azathioprine (Aza) and Pr (n=8), and of healthy subjects (n=12). PMNLs of CyA- and Aza-treated transplant patients displayed markedly higher gelatinase content (2427±489 and 3284±357 ng/107 cells) than PMNLs of controls (528±83 ng/107 cells). There was also a higher content of type I collagenase in PMNLs (3374±292 ng/107 cells) of Aza-treated patients and significantly elevated levels in PMNLs of patients receiving CyA (3625±229 ng/107 cells) compared with healthy subjects (2878±151 ng/107 cells). In contrast, neutrophil lactoferrin content was lower in transplant patients. Thus, immunosuppressive drugs may reduce the release of leukocyte proteinases, which are known for their deleterious role in proteolytic tissue and matrix breakdown. In vitro, the effects of different immunosuppressive drugs on the release of lactoferrin, collagenase and gelatinase were investigated on FMLPNTL-stimulated PMNLs isolated from healthy subjects. CyA but not Aza or Pr caused inhibition of gelatinase, collagenase and lactoferrin release.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00335512
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