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  • General Chemistry  (12)
  • Analytical Chemistry and Spectroscopy  (10)
  • Eastern Pacific Ocean  (3)
  • Cobalt(III) porphyrins  (2)
  • Drosophila  (2)
  • 1
    Electronic Resource
    Electronic Resource
    The Hermes element from Musca domestica can transpose in four families of cyclorrhaphan flies (1997)
    Springer
    Genetica 99 (1997), S. 15-29 
    Details
    ISSN: 1573-6857
    Keywords: Drosophila ; Musca ; recombination ; transgenics ; transposable elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic insect technology will provide opportunities to explore the basic biology of a broad range of insect species in ways that will prove insightful and important. It is also a technology that will provide opportunities to manipulate the genotypes of insects of practical significance to the health and welfare of humans. The Hermes transposable element from the housefly, Musca domestica, is a short inverted repeat-type element related to hobo from Drosophila melanogaster, Ac from Zea mays, and Tam3 from Antirrhinum majus. It has potential to become a versatile and efficient broad host-range insect transformation vector. The ability of Hermes to transpose when introduced into five species of diptera from four divergent families was tested using an in vivo, interplasmid transpositional recombination assay. Hermes was capable of transposing in all species tested, demonstrating that Hermes has a broad host-range. In addition, the rates of transposition were sufficiently high in all species tested to suggest that Hermes will be an efficient gene transfer vector in a wide range of insect species. The Hermes element also revealed a pattern of integration into the target substrate that permitted factors determining integration site selection to be identified. Primary nucleotide sequence of the integration site played a role as did proximity to preferred integration sites and the nucleosomal organization of the target.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018349803656
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  • 2
    Electronic Resource
    Electronic Resource
    TheHermes element fromMusca domestica can transpose in four families of cyclorrhaphan flies (1997)
    Springer
    Genetica 99 (1997), S. 15-29 
    Details
    ISSN: 1573-6857
    Keywords: Drosophila ; Musca ; recombination ; transgenics ; transposable elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic insect technology will provide opportunities to explore the basic biology of a broad range of insect species in ways that will prove insightful and important. It is also a technology that will provide opportunities to manipulate the genotypes of insects of practical significance to the health and welfare of humans. TheHermes transposable element from the housefly,Musca domestica, is a short inverted repeat-type element related tohobo fromDrosophila melanogaster, Ac fromZea mays, andTam3 fromAntirrhinum majus. It has potential to become a versatile and efficient broad host-range insect transformation vector. The ability ofHermes to transpose when introduced into five species of diptera from four divergent families was tested using anin vivo, interplasmid transpositional recombination assay.Hermes was capable of transposing in all species tested, demonstrating thatHermes has a broad host-range. In addition, the rates of transposition were sufficiently high in all species tested to suggest thatHermes will be an efficient gene transfer vector in a wide range of insect species. TheHermes element also revealed a pattern of integration into the target substrate that permitted factors determining integration site selection to be identified. Primary nucleotide sequence of the integration site played a role as did proximity to preferred integration sites and the nucleosomal organization of the target.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02259495
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of cholesterol, cholesterol-5,6-epoxides and cholestane-3β,5α,6β-triol in nipple aspirates of human breast fluid by gas chromatography/mass spectrometry (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 14 (1987), S. 335-338 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method has been developed for the quantitative determination of cholesterol and three of its major oxidative metabolites (the 5α,6α-epoxide, the 3β,5α,6β-triol, and the 5β,6β-epoxide) in a single sample of human breast fluid (2-50 μl), using gas chromatography/mass spectrometry with selected ion monitoring. High specificity and reliable quantification is achieved by the use of the inverse stable isotope dilution method, employing deuterium-labeled variants of the compounds as internal standards.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200140707
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  • 4
    Electronic Resource
    Electronic Resource
    Synthese von Alkinen aus Enolestern (1969)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 81 (1969), S. 437-446 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Dieser Aufsatz gibt einen Überblick über Eliminierungen, die von Enolsulfonaten, Enolphosphaten und Enolphosphoniumsalzen zu Alkinen führen. Bei der Bildung der Dreifachbindung ist gewöhnlich die Eliminierung am Olefin der geschwindigkeitsbestimmende Schritt. Enolester können möglicherweise auch in der Biosynthese der Alkine eine Rolle spielen.
    Additional Material: 6 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19690811202
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  • 5
    Electronic Resource
    Electronic Resource
    Ein Modell für die Biosynthese von Acetylenen und Allenen (1964)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 76 (1964), S. 794-794 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19640761839
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  • 6
    Electronic Resource
    Electronic Resource
    Isolierung und Stereochemie des antibakteriellen Prinzips aus Haematoxylon braziletto (1964)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 76 (1964), S. 794-794 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19640761838
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  • 7
    Electronic Resource
    Electronic Resource
    An improved selected ion recording system for precise isotope ratio determination (1980)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1980), S. 381-384 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An improved analog four-channel selected ion recording system is described, in which major modifications permit a decrease in the dwell time to 33 ms per channel, thus minimizing the mass cycling error. Synchronization with a sinusoidal sweep voltage superimposed on the normal accelerating voltage (8 kV) enables two channels to be monitored simultaneously in real time and each mass to be recorded continuously. These improvements allow measurement of ion current ratios with a precision of 0.2% over a wide dynamic range, permitting accurate determination of isotopic enrichment in biomedical assays even when this enrichment is derived from a single label. Use of the system is illustrated by the analysis of palmitate turnover in dogs (using [1 - 13C] palmitic acid) with an average standard deviation corresponding to the detection of 0.04% excess of [1 - 13C] palmitate.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200070905
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  • 8
    Electronic Resource
    Electronic Resource
    Multiple ion detection by accelerating voltage alternation in conjunction with voltage sweeping (1974)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 1 (1974), S. 418-422 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An analog multiple ion detection system has been developed for magnetic sector mass spectrometers based on accelerating voltage alternation in conjunction with the accurate maintenance of focusing by means of voltage sweeping, using a superimposed a.c. sine wave voltage. This multiple ion detector voltage sweep system is easily constructed, inexpensive and produces analytical data of much greater precision than that reported from conventional systems. The application of the multiple ion detector voltage sweep system to biomedical problems is illustrated by the development of a method for the determination of stable isotope enrichment in blood glucose and alanine in children infused with tetradeuterio-L-alanine and dideuterioglucose to study gluconeogenesis and glucose utilization in vivo.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200010610
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  • 9
    Electronic Resource
    Electronic Resource
    Determination of chlorpromazine and its major metabolites by gas chromatography/mass spectrometry: Application to biological fluids (1985)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 707-713 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method for the quantitative determination of chlorpromazine and five of its major metabolites in a single sample of biological fluid in the ng/ml range has been developed utilizing gas chromatography/mass spectrometry with selected ion recording. The assay is highly specific and quantification is accomplished by an inverse stable isotope dilution technique, using deuterium-labeled variants of the compounds as internal standards. In this way the concentrations of chlorpromazine and five of its major metabolites (the sulfoxide, the N-oxide, the monodemethylated, the didemethylated, and the 7-hydroxylated compounds) can be determined in biological fluids. Levels in humans have been measured both in plasma and in red blood cells and are compared to those found in related in vitro studies.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200121207
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  • 10
    Electronic Resource
    Electronic Resource
    A stable isotope technique for investigating lactate metabolism in humans (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 458-462 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A stable isotope tracer method has been developed for studying lactate metabolism in humans. The method uses lactic acid triply labeled with 13C as the tracer. The stable isotope is infused to attain a level of approximately 1.5% of that of the circulating unlabeled lactate. Following the isolation of lactic acid from the blood, the percentage of triply labeled (13C)lactate is measured using gas chromatography mass spectrometry. We compared this method with tracer methodology using [14C]lactate and found comparable results.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100804
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