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    Deposition of biogenic iron minerals in a methane oxidizing microbial mat. (2018)
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    Publication Date: 2021-03-29
    Description: The syntrophic community between anaerobic methanotrophic archaea and sulfate reducing bacteria forms thick, black layers within multi-layered microbial mats in chimney-like carbonate concretions of methane seeps located in the Black Sea Crimean shelf. The microbial consortium conducts anaerobic oxidation of methane, which leads to the formation of mainly two biomineral by-products, calcium carbonates and iron sulfides, building up these chimneys. Iron sulfides are generated by the microbial reduction of oxidized sulfur compounds in the microbial mats. Here we show that sulfate reducing bacteria deposit biogenic iron sulfides extra- and intracellularly, the latter in magnetosome-like chains. These chains appear to be stable after cell lysis and tend to attach to cell debris within the microbial mat. The particles may be important nuclei for larger iron sulfide mineral aggregates.
    Keywords: Biogenic Iron Minerals; Methane; Microbial Mat ; 551 ; Anaerobiosis ; Archaea ; Bacteria ; Black Sea ; Calcium Carbonate ; Geologic Sediments ; Iron ; Methane ; Microbial Consortia ; Oxidation-Reduction ; Seawater ; Sulfates ; Sulfides ; Sulfur-Reducing Bacteria
    Language: English , English
    Type: article , publishedVersion
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    CITATION GEO-LEO
  • 2
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    Localization of Methyl-Coenzyme M reductase as metabolic marker for diverse methanogenic Archaea. (2018)
    Details
    Publication Date: 2021-03-29
    Description: Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated. Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50-70% of the enzyme was localized close to the cytoplasmic membrane, which implies "facultative" membrane association of the enzyme. This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms.
    Description: Open-Access-Publikationsfonds 2013
    Keywords: Methyl-Coenzyme M Reductase; Metabolic Marker; Methanogenic Archaea ; 551 ; Anaerobiosis ; Antibodies ; Archaea ; Cell Membrane ; Cytoplasm ; Methane ; Oxidation-Reduction ; Oxidoreductases ; Staining and Labeling
    Language: English , English
    Type: article , publishedVersion
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    CITATION GEO-LEO
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