ISSN:
1040-0397
Keywords:
Peroxidases
;
Hydrogen peroxide
;
Carbon paste electrodes
;
Amperometric
;
Organic-phase biosensor
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Biosensor characteristics of peroxidase-lactitol-modified carbon paste microclectrodes have been evaluated in aqueous and partial aqueous media (H2O-10% v./v. CH3OH and H2O-10% v./v. CH3CN), for hydrogen peroxide and 2-butanone peroxide amperometric detection, using a flow injection system. The activity of the investigated peroxidases (horseradish and fungal peroxidase from Arthromyces ramosus) was higher for hydrogen peroxide than for 2-butanone peroxide, irrespective of the solvent composition. For both peroxidases, the microelectrodes' sensitivity, estimated from the slopes of the linear domains as well as calculated from the Eddie-Hofstee plots decreased in the sequence: H2O 〉 H2O-10% v./v. CH3CN 〉 H2O-10% v./v. CH3OH. It is suggested that this behavior was due to the difference between the polarity and other physical properties, such as kinematic viscosity and dielectric constant of the nonaqueous solvents.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140081107
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