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  • 1
    Electronic Resource
    Electronic Resource
    Inactivation and unfolding of aminoacylase during denaturation in sodium dodecyl sulfate solutions (1995)
    Springer
    The protein journal 14 (1995), S. 349-357 
    Details
    ISSN: 1573-4943
    Keywords: Aminoacylase ; sodium dodecyl sulfate ; inactivation ; conformational change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract During denaturation by sodium dodecyl sulfate (SDS), aminoacylase shows a rapid decrease in activity with increasing concentration of the detergent to reach complete inactivation at 1.0 mM SDS. The denatured minus native-enzyme difference spectrum showed two negative peaks at 287 and 295 nm. With the increase of concentration of SDS, both negative peaks increased in magnitude to reach maximal values at 5.0 mM SDS. The fluorescence emission intensity of the enzyme decreased, whereas there was no red shift of emission maximum in SDS solutions of increasing concentration. In the SDS concentration regions employed in the present study, no marked changes of secondary structure of the enzyme have been observed by following the changes in far-ultraviolet CD spectra. The inactivation of this enzyme has been followed and compared with the unfolding observed during denaturation in SDS solutions. A marked inactivation is already evident at low SDS concentration before significant conformational changes can be detected by ultraviolet absorbance and fluorescence changes. The inactivation rate constants of free enzyme and substrate-enzyme complex were determined by the kinetics method of the substrate reaction in the presence of inactivator previously described by Tsou [Tsou (1988),Adv. Enzymol. Related Areas Mol. Biol. 61, 381–436]. It was found that substrate protects against inactivation and at the same SDS concentrations, the inactivation rate of the free enzyme is much higher than the unfolding rate. The above results show that the active sites of metal enzyme containing Zn2+ are also situated in a limited and flexible region of the enzyme molecule that is more fragile to denaturants than the protein as a whole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01886792
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  • 2
    Electronic Resource
    Electronic Resource
    Radiation crosslinked poly(vinylmethylsiloxane) for levonorgestrel delivery system (1988)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Letters Edition 26 (1988), S. 195-199 
    Details
    ISSN: 0887-6258
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1988.140260405
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  • 3
    Electronic Resource
    Electronic Resource
    Thermal and mechanical properties of phenolphthalein polyethersulfone/poly(phenylene sulfide) blends (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 55 (1995), S. 633-639 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The thermal and mechanical properties of phenolphthalein polyethersulfone/poly(phenylene sulfide) (PES-C/PPS) blends were studied using a differential scanning calorimeter, a dynamic mechanical analyzer, and mechanical characterization. The morphologies of fracture surfaces were observed by scanning electron microscopy. The blends are multiphase systems with strong interaction between the two phases. It is of interest that, although the strength and ductility of PPS are lower than those of PES-C, the addition of PPS can improve markedly the impact strength of PES-C without changing its higher strength. The PPS can also act as a flow aid for PES-C. © 1995 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070550411
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of metal salts on poly(DL-lactide-co-glycolide) polymer hydrolysis (1997)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 34 (1997), S. 531-538 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The effects of encapsulated metal salts on poly(DL-lactide-co-glycolide) (PLGA) water uptake and degradation properties were investigated in this work. Salts of varying aqueous solubility characteristics were incorporated into PLGA films either as particles or by codissolution in polymer solutions. Polymer films were characterized with respect to the kinetics of water uptake, morphology changes, degradation, and weight loss after hydration. It was found that these properties are strongly influenced by the presence and nature of encapsulated salts. Effects range from minor changes in water uptake profile with no significant difference in degradation kinetics to major alterations in water uptake kinetics together with a several-fold decrease in the polymer degradation rate. Possible mechanistic explanations for the observed effects are discussed. © 1997 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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