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  • 1
    Electronic Resource
    Electronic Resource
    Inactivation and unfolding of aminoacylase during denaturation in sodium dodecyl sulfate solutions (1995)
    Springer
    The protein journal 14 (1995), S. 349-357 
    Details
    ISSN: 1573-4943
    Keywords: Aminoacylase ; sodium dodecyl sulfate ; inactivation ; conformational change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract During denaturation by sodium dodecyl sulfate (SDS), aminoacylase shows a rapid decrease in activity with increasing concentration of the detergent to reach complete inactivation at 1.0 mM SDS. The denatured minus native-enzyme difference spectrum showed two negative peaks at 287 and 295 nm. With the increase of concentration of SDS, both negative peaks increased in magnitude to reach maximal values at 5.0 mM SDS. The fluorescence emission intensity of the enzyme decreased, whereas there was no red shift of emission maximum in SDS solutions of increasing concentration. In the SDS concentration regions employed in the present study, no marked changes of secondary structure of the enzyme have been observed by following the changes in far-ultraviolet CD spectra. The inactivation of this enzyme has been followed and compared with the unfolding observed during denaturation in SDS solutions. A marked inactivation is already evident at low SDS concentration before significant conformational changes can be detected by ultraviolet absorbance and fluorescence changes. The inactivation rate constants of free enzyme and substrate-enzyme complex were determined by the kinetics method of the substrate reaction in the presence of inactivator previously described by Tsou [Tsou (1988),Adv. Enzymol. Related Areas Mol. Biol. 61, 381–436]. It was found that substrate protects against inactivation and at the same SDS concentrations, the inactivation rate of the free enzyme is much higher than the unfolding rate. The above results show that the active sites of metal enzyme containing Zn2+ are also situated in a limited and flexible region of the enzyme molecule that is more fragile to denaturants than the protein as a whole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01886792
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  • 2
    Electronic Resource
    Electronic Resource
    Eigenproperties of large-scale structures by finite element partitioning and homotopy continuation (1989)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 28 (1989), S. 2113-2122 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Finite element partitioning (or substructuring) is employed to estimate the eigenproperties of large-scale structural systems. A homotopy equation is constructed and its solutions are characterized by a number of curves which connect the eigensolutions of the partitions with those of the complete system. A step-by-step tracing procedure is developed to follow these curves. At each step, prediction and correction are performed. The Rayleigh-Ritz procedure and the conjugate gradient method are used as predictor and corrector, respectively. Compared with the sole use of either the Rayleigh-Ritz or gradient methods, the proposed method is more reliable and more efficient for large-scale problems. Numerical implementation is well suited for supercomputers.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620280909
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  • 3
    Electronic Resource
    Electronic Resource
    Multiple scale finite element methods (1991)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 32 (1991), S. 969-990 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: New temporal and spatial discretization methods are developed for multiple scale structural dynamic problems. The concept of fast and slow time scales is introduced for the temporal discretization. The required time step is shown to be dependent only on the slow time scale, and therefore, large time steps can be used for high frequency problems. To satisfy the spatial counterpart of the requirement on time step constraint, finite-spectral elements and finite wave elements are developed. Finite-spectral element methods combine the usual finite elements with the fast convergent spectral functions to obtain a faster convergence rate; whereas, finite wave elements are developed in parallel to the temporal shifting technique. Therefore, the spatial resolution is increased substantially. These methods are especially applicable to structural acoustics and linear space structures. Numerical examples are presented to illustrate the effectiveness of these methods.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620320504
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