GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (1)
  • nicotinamide  (1)
  • Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; CTD, Sea-Bird SBE 911plus; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; EXP; Experiment; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hata_etal_02; LICOR quantameter (LI-COR LI-192SA); North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Precision analyzer (Model EN-501A, Kimoto Electric); Radiation, photosynthetically active; Rocky-shore community; Salinity; Salinometer, inductive; Shiraho coral reef; Temperate; Temperature, water
Document type
Source
Keywords
  • nicotinamide  (1)
  • Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; CTD, Sea-Bird SBE 911plus; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; EXP; Experiment; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hata_etal_02; LICOR quantameter (LI-COR LI-192SA); North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Precision analyzer (Model EN-501A, Kimoto Electric); Radiation, photosynthetically active; Rocky-shore community; Salinity; Salinometer, inductive; Shiraho coral reef; Temperate; Temperature, water
  • Chinese hamster ovary (CHO) cells  (1)
  • Rubisco activase  (1)
  • ascorbic acid  (1)
    • +
Publisher
Years
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Utilization and stability of vitamins in serum-containing and serum-free media in CHO cell culture (1990)
    Springer
    Cytotechnology 4 (1990), S. 243-250 
    Details
    ISSN: 1573-0778
    Keywords: Chinese hamster ovary (CHO) cells ; vitamins ; ascorbic acid ; choline ; nicotinamide ; thiamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The concentrations of four vitamins, ascorbic acid, nicotinamide, choline and thiamine were evaluated in the culture supernatant of Chinese hamster ovary (CHO) cells. The media used were α-modified Eagle's minimum essential medium (MEM-α) supplemented with 10% fetal calf serum, and a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (DME/F12), containing neither serum nor protein. The reference experiment without cells revealed instability of ascorbic acid and thiamine. Moreover, a significant amount of each vitamin decreased in the culture supernatant. The possibility of growth limitation by vitamin depletion is strongly suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00563784
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...