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  • 1
    Electronic Resource
    Electronic Resource
    Phenotype and hormonal status of transgenic tobacco plants overexpressing the rolA gene of Agrobacterium rhizogenes T-DNA (1993)
    Springer
    Plant molecular biology 23 (1993), S. 1199-1210 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium ; growth retardants ; plant hormones ; Ri plasmid ; transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rolA gene of the TL-DNA of Agrobacterium rhizogenes Ri-plasmid plays a major role in establishing the hairy root syndrome in transgenic plants. Transgenic tobacco plants (Nicotiana tabacum L.) expressing constitutively the rolA gene under the transcriptional control of the 35S RNA promoter show pronounced phenotypical alterations. P35S-rolA transgenic tobacco plants are characterized by stunted growth, dark green wrinkled leaves with an altered length-to-width ratio, condensed inflorescences, retarded onset of flowering, a reduced number of flowers and shortened styles. To investigate whether the pleiotropic alterations of growth and development are linked to an altered hormonal status we have compared the immunoreactive content of indole-3-acetic acid, cytokinins, abscisic acid, gibberellin and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) of seedlings and different tissues of P35S-rolA transgenic plants, transgenic plants expressing the rolA gene under control of its own phloem-specific promoter and wild-type plants. Multiple tissue-specific alterations of phytohormone concentrations are the consequence of rolA gene activity. Changes of phytohormonal content can explain part of the rolA-induced phenotypic alterations. Most strikingly, in young and fully developed leaves of rolA and P35S-rolA transgenic clones a 40–60% reduction of immunoreactive gibberellin A1 was found, as compared to wild-type leaves. Treatment of wild-type tobacco plants with inhibitors of gibberellin biosynthesis phenotypic alterations similar to those of rolA transgenic plants. This suggests that the reduction of gibberellic acid content is indirectly but causally involved in rolA-induced alterations of stem elongation and planar leaf blade growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042353
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of host range determinants in the Rhizobium species MPIK3030 (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 42-48 
    Details
    ISSN: 1617-4623
    Keywords: Host-range ; Tn5 mutagenesis ; hsn ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary R. meliloti primarily nodulates Medicago sativa but cannot nodulate Macroptilium atropurpureum. By introducing an 11.4 kb region into R. meliloti from the Symplasmid of Rhizobium strain MPIK3030, the host range of the R. meliloti transconjugants were shown to be extended to M. atropurpureum, one of the hosts of MPIK3030 but not normally nodulated by R. meliloti. The region responsible for host range extension was isolated by mass conjugating a clone bank from MPIK3030 into the R. meliloti wild type, and subsequent screening for nodulation on M. atropurpureum. Using deleted derivatives of a plasmid reisolated from endosymbiotic bacteria, the host range region was further narrowed down to three EcoRI fragments. Tn5 mutagenesis allowed the isolation of three discrete regions on an 11.4 kb section, which are involved in the extension of host range to M. atropurpureum. Finally, complementation experiments performed with R. meliloti common nod and hsn mutants indicated that none of the genes involved in the early steps of nodulation, including host-range functions, can be complemented by genes carried on the 11.4 kb fragment derived from MPIK3030.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330382
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  • 3
    Electronic Resource
    Electronic Resource
    Differential regulation of soybean chalcone synthase genes in plant defence, symbiosis and upon environmental stimuli (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 315-322 
    Details
    ISSN: 1617-4623
    Keywords: Multigene family ; Clustered genes ; Promoter region ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four independent recombinant λ clones hybridizing to parsley chalcone synthase (CHS) cDNA were isolated from a soybean (Glycine max) genomic library. Restriction fragment length polymorphism (RFLP) analysis indicated that the CHS gene family comprises six members. The CHS genes were found to be clustered with three genes on a 10 kb segment and pairs on others. DNA sequences of the 5′-, the coding-, and the 3′ untranslated regions were determined for three different genes. A consensus alignment of the 5′ regions revealed extensive homology between them for up to 150 bp upstream of the TATA box. Developmental regulation of CHS was observed in uninfected and in rhizobium-infected roots. Regulation at the level of transcription by different stimuli was investigated in the root, stem and cotyledons of soybean seedlings. Our results suggest a co-operative induction of CHS genes by wounding and clicitor treatment of cotyledons. The most rapid transcript accumulation, however, was observed in roots and stems. The induction of CHS genes by light was found to be UV dependent. A possible involvement of different members of the CHS gene family in response to elicitor versus UV treatment was analysed by the use of gene specific probes, and unexpectedly revealed that only CHS 1 transcription was induced by either elicitor or UV treatment of seedlings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331284
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  • 4
    Electronic Resource
    Electronic Resource
    The Rhizobium meliloti early nodulation genes (nodABC) are nitrogen-regulated: Isolation of a mutant strain with efficient nodulation capacity on alfalfa in the presence of ammonium (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 89-96 
    Details
    ISSN: 1617-4623
    Keywords: Nitrogen regulation (ntr) ; nodABC ; nodD3 ; Nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The presence of combined nitrogen in the soil suppresses the formation of nitrogen-fixing root nodules by Rhizobium. We demonstrate that bacterial genes determining early nodulation functions (nodABC) as well as the regulatory gene nodD3 are under nitrogen (NH 4 + ) control. Our results suggest that the gene product of nodD3 has a role in mediating the ammonia regulation of early nod genes. The general nitrogen regulatory (ntr) system as well as a chromosomal locus mutated in Rhizobium meliloti were also found to be involved in the regulation of nod gene expression. A R. meliloti mutant with altered sensitivity to ammonia regulation was isolated, capable of more efficient nodulation of alfalfa than the wild-type strain in the presence of 2 mM ammonium sulfate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261162
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a light-inducible, organ-specific gene from potato and analysis of its expression after tagging and transfer into tobacco and potato shoots (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 14-22 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Light induction ; Position effect ; Transformed plants ; Gene isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The isolation and analysis of several cDNAs and of one genomic clone encoding ST-LS1, a single-copy gene fromSolanum tuberosum with leaf/stem-specific, light-inducible expression, is described. The structure of the gene was determined by sequencing several overlapping partial cDNA clones as well as the genomic clone and by determining the transcription start site by RNA protection experiments. A “tagged” derivative of the gene, obtained by exon modification, was reintroduced into potato and into tobacco shoots usingAgrobacterium/Ti-plasmid vector systems. The modified gene was expressed in both tobacco and potato shoots giving rise to an RNA of approximately 1,200 nucleotides which exceeded the length of the RNA made from the endogenous gene by the expected size of the “tag” (470 nucleotides). The level of expression of the modified gene varied substantially between independent transformants. A high proportion of the transformants (20%–40%) synthesized as much RNA from the added gene as from the resident gene. Expression of the transferred gene was light induced. Qualitatively and quantitatively the expression of the introduced gene was similar in a homologous (potato) and in a heterologous, but related, cellular background (tobacco).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02428027
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