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  • 1
    Keywords: Climatic changes ; Forests and forestry ; Climatic changes ; Forests and forestry ; Agriculture ; Agriculture ; Climate Change ; Forestry management.
    Description / Table of Contents: This book collects wide-ranging contributions such as case studies, reviews, reports on technological developments, outputs of research/studies, and examples of successful projects, presenting current knowledge and raising awareness to help the agriculture and forestry sectors find solutions for mitigating climate variability and adapting to change. It brings the topic of ecosystem services closer to education and learning, as targeted by the Framework Convention on Climate Change and the Paris Agreement, the 2030 Agenda for Sustainable Development and the EU Biodiversity Strategy to 2020. Climate change and its impacts on agriculture and agroforestry have been observed across the world during the last 50 years. Increasing temperatures, droughts, biotic stresses and the impacts of extreme events have continuously decreased agroforestry systems’ resilience to the effects of climate change. As such, there is a need to adapt farming and agroforestry systems so as to make them better able to handle ever-changing climate conditions, and to preserve habitats and ecosystems services
    Type of Medium: Online Resource
    Pages: Online-Ressource (X, 520 p, online resource)
    Edition: Springer eBook Collection. Earth and Environmental Science
    ISBN: 9783319750040
    Series Statement: Climate Change Management
    Language: English
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  • 2
    ISSN: 1573-0778
    Keywords: BSA ; CHO cells ; interferon-γ ; linoleic acid ; lipids ; Pluronic F68
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The role of bovine serum albumin in mammalian cell cultures and the possibility of its substitution by other components in a serum-free medium has been investigated. In this study, BSA was shown to be important for growth and product formation in CHO cells expressing recombinant human interferon-γ. There were indications that its stimulating growth effect was dependent on the source of BSA used and probably was related to the purification procedure used for the production of the desired albumin fraction. Cell growth did not occur in the absence of BSA but at low concentration (1 mg ml−1) it was stimulated by the addition of a combination of a commercial lipid mixture plus Pluronic F68. However, under the latter conditions IFN-γ production was adversely effected. The importance of individual lipid components was investigated using a statistical approach based on a Plackett-Burman design. Linoleic acid was identified as a positive variable for cell growth while cholesterol was identified as a negative variable for both cell growth and IFN-γ production. When a combination of linoleic acid plus Pluronic F68 was included in the formulation of low BSA medium, cell growth was similar to that at high BSA concentration (5 mg ml−1) but the IFN-γ concentration was significantly reduced (ca. 45%).
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-0778
    Keywords: Glycosylation ; recombinant ; interferon ; CHO cells ; lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effects of lipids on the glycosylation of recombinant human interferon-γ expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-glycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-γ deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-γ glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.
    Type of Medium: Electronic Resource
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