ISSN:
1615-6102
Keywords:
Actin
;
Cauliflower
;
Cortical cytoskeleton
;
Membrane cytoskeleton
;
Plant plasma membrane
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well defined, inside-out and right-side-out plasma membrane vesicles can be used to study the structure and dynamics of the plant cortical cytoskeleton.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01403398
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