Publication Date:
2024-03-15
Description:
Ocean acidification (OA) is predicted to play a major role in shaping species biogeography and marine biodiversity over the next century. We tested the effect of medium-term exposure to OA (pH 8.00, 7.30 and 6.70 for 30 d) on acid-base balance in the decapod crab Necora puber-a species that is known to possess good extracellular buffering ability during short-term exposure to hypercapnic conditions. To determine if crabs undergo physiological trade-offs in order to buffer their haemolymph, we characterised a number of fundamental physiological functions, i.e. metabolic rate, tolerance to heat, carapace and chelae [Ca2+] and [Mg2+], haemolymph [Ca2+] and [Mg2+], and immune response in terms of lipid peroxidation. Necora puber was able to buffer changes to extracellular pH over 30 d exposure to hypercapnic water, with no evidence of net shell dissolution, thus demonstrating that HCO3- is actively taken up from the surrounding water. In addition, tolerance to heat, carapace mineralization, and aspects of immune response were not affected by hypercapnic conditions. In contrast, whole-animal O2uptake significantly decreased with hypercapnia, while significant increases in haemolymph [Ca2+] and [Mg2+] and chelae [Mg2+] were observed with hypercapnia. Our results confirm that most physiological functions in N. puber are resistant to low pH/hypercapnia over a longer period than previously investigated, although such resistance comes at the expenses of metabolic rates, haemolymph chemistry and chelae mineralization.
Keywords:
Acid-base regulation; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Arthropoda; Atomic absorption spectrometer (Spectr AA 600, Varian); Automated CO2 analyzer (CIBA-Corning 965, UK); Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcification/Dissolution; Calcite saturation state; Calcite saturation state, standard deviation; Calculated, see reference(s); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure, standard deviation; Coast and continental shelf; Comment; Conductivity meter (WTW, Weilheim, Gemany); EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Identification; Laboratory experiment; Necora puber; Necora puber, carapace, calcium; Necora puber, carapace, magnesium; Necora puber, chelae, calcium; Necora puber, chelae, magnesium; Necora puber, haemolymph, calcium ion; Necora puber, haemolymph, lipid peroxide; Necora puber, haemolymph, magnesium ion; Necora puber, haemolymph, partial pressure of carbon dioxide; Necora puber, haemolymph, pH; Necora puber, haemolymph, total carbon; Necora puber, mass; Necora puber, width; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Oxygen consumption; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; pH meter (Mettler Toledo, USA); Respiration; Salinity; Salinity, standard deviation; see reference(s); Sex; Single species; Strathkelvin oxygen electrode system; Temperate; Temperature, standard deviation; Temperature, water; Thermal limit
Type:
Dataset
Format:
text/tab-separated-values, 1687 data points
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