Description: Anthropogenic nutrient enrichment is changing the structure and the function of coastal ecosystems. These coastal zones are transitions between freshwater and marine systems where multiple biogeochemical processes remove, produce and transform organic matter. The extent to which the coastal zone is merely a conduit for terrestrial (allochthonous) organic matter, versus a distinct source of autochthonous organic matter fueled by eutrophication, is unclear. To address this issue, we characterized the freshwater and marine dissolved organic matter (DOM) pools in an eutrophic estuary with a long water residence time (Roskilde Fjord, Denmark) over an annual cycle. We combined elemental, optical (absorbance and fluorescence) and isotopic analyses to obtain insight about the bulk properties of the DOM pool during this period. We also used sediment traps to analyze the changes related to the exchange of organic matter between the particulate (POM) and dissolved (DOM) fractions. The results showed that labile autochthonous DOM from in situ primary production was rapidly transformed to more recalcitrant DOM that accumulated in the estuary despite continuous exchange with the open sea. Also, parts of the POM pool were degraded rapidly (within 24h) and transformed into the DOM pool. Accumulated DOM was characterized by relatively low molecular size and stable carbon isotopic value, and by high protein-like fluorescence. These results indicate that autotrophic material can be a major source of specific recalcitrant DOM in eutrophic coastal waters, contributing significantly to the flux of organic carbon to the ocean.

Description: Two stations were visited in the western tropical Pacific Ocean: The South-East Asia Time-series Station (SEATS) in the northern South China Sea (SCS) and station F2 in the Philippine Sea, ~230 km to the northeast of the Luzon Strait. Water samples were taken aboard the R/V Dong-Fang-Hong 2 in July and August 2017 from 13 depths between 5 m and 3800 m at SEATS and 11 depths between 5 m and 3200 m at F2 using Niskin bottles mounted on a SeaBird 911 conductivity-temperature-depth (CTD) rosette sampler. A time-course irradiation with sampling time at 0, 1, 3, 5, 7, and 10 d was conducted for the bottommost samples from SEATS and F2. The value of different parameters was calculated as the ratio of the signal of property X at irradiation time t to that at time zero.