ISSN:
0170-2041
Keywords:
Aborycin
;
Peptides
;
Antibiotics
;
Streptomyces griseoflavus TÜ 4072
;
HIV
;
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The screening for growth inhibitors against Bacillus subtilis revealed a peptide antibiotic produced by Streptomyces griseoflavus TÜ 4072. Very high yields of the antibiotic named aborycin were obtained by cultivation in a medium containing soybean meal and sucrose. Isolation from the mycelium was carried out by a combination of extraction and gel chromatography. Preparative HPLC yielded the uniform antibiotic. The structure elucidation was based on GC amino acid analysis on a chiral phase, automated Edman degradation, electrospray tandem mass spectrometry and two-dimensional NMR (TOCSY, ROESY, HSQC, HMBC). Aborycin represents a 21-peptide that is cyclized from the side chain of Asp9 to the N-terminus of Cys1. Two disulfide bonds Cys1 to Cys13 and Cys7 to Cys19 form a tricyclic structure consisting exclusively of protein amino acids. The peptide antibiotic aborycin was found to be identical with a HIV protease inhibitor isolated recently from Streptomyces SP 9440.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jlac.199419940716
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