Publication Date:
2023-02-23
Description:
Physicochemical parameters and relative abundance of selected nutrient cycling genes were measured in sediment and pore water samples taken from a fish farming area (Site S3 16◦23.097 N, 119◦55.551 E, depth 12.6m) and a control site (Site S1 16◦22.960 N, 119◦54.723 E, depth 15m) not influenced by fish farming. From each site, five pairs of cores representing five biological replicates were collected. One core from each pair was used for physicochemical analysis while the other cores were used for microbial community analysis. Samples from S1 were collected on 2017-06-10 and samples from S3 were collected the following day.
Nitrogen (bacterial ammonia monooxygenase/ amoA and nitrite reductase/ nirK) and sulfur (dissimilatory sulfate reductase/ dsrA and sulfur oxidation/ soxB) cycling genes were quantified and normalized against the abundance of the 16S rRNA gene.
Keywords:
ACUTE; Ammonia monooxygenase, amoA gene, relative abundance; Aquaculture practice in tropical coastal ecosystems - understanding ecological and socio-economic consequences; Core; DHC; Dissimilatory sulfate reductase, dsrA gene, relative abundance; Diver-held corer; Event label; Extraction: Mobio DNA Isolation Kit (Mo Bio Laboratories, Carlsbad, USA); Analysis: Shimadzu Biospec Nano microvolume spectrophotometer; FC; fish cage; Guiguiwanen_channel_S1; Guiguiwanen_channel_S3; marine aquaculture; Nitrite reductase, nirK gene, relative abundance; nutrient loading; OC; off cage site; Philippines; Site; Sulfur oxidizing multi-enzyme, gene soxB, relative abundance
Type:
Dataset
Format:
text/tab-separated-values, 140 data points
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