ISSN:
1432-1912
Keywords:
Phalloidin poisoning
;
Isolated hepatocytes
;
4,4′-diisothiocyano stilbene-2,2′-disulfonic acid
;
Plasma membranes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 4,4′-Diisothiocyano stilbene-2,2′-disulfonic acid (DIDS) inhibits the typical development of protrusions, regularly seen after treatment of isolated hepatocytes with phalloidin. The degree of inhibition depends on the time of preincubation and on the concentration of DIDS, but not on the concentration of phalloidin. DIDS is more effective than H2DIDS. The inhibition by both compounds is irreversible. The binding capacity of hepatocytes for H2DIDS is much higher than that of the phalloidin-insensitive hepatoma cells. Gel electrophoresis of lysates from cells, pretreated with 3H2DIDS demonstrates that actin binds very little of the inhibitor. Our results suggest that a protein structure on the surface of hepatocytes, needed for the response to phalloidin, is influenced by DIDS or H2DIDS.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00507973
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