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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 77 (1976), S. 79-89 
    ISSN: 1437-1596
    Keywords: Blutalkoholabbau im Schock ; Schock ; Alkoholabbau
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Nach einer Alkoholbelastung von 3 g Äthanol pro Kilogramm reduziertem Körpergewicht und nach einem 1-stündigen Diffusionsausgleich wurde unter einer Halothan-N2O/O2-Narkose an Bastardhunden unter standardisierten Bedingungen ein Blutverlust von 30 bis 40 % der Gesamtblutmenge erzeugt. Über eine Versuchsdauer von 3 Stunden wurden alle 30 Minuten BAK-Bestimmungen nach der ADH-Methode durchgeführt. Gegenüber der Kontrolltieren fand sich bei den Versuchstieren im hämorrhagischen Schock im vergleichbaren Beobachtungszeitraum eine signifikante Verzögerung des mittleren Gesamtabbaus p = 0,005 (U-Test mit Rangauflistung).
    Notes: Summary Ethanol at a dosage of 3 g/kg reduced body weight was injected i.v. into mongrel dogs resulting in a blood alcohol concentration of approximately 2.9 mg/ml. One hour after injection the dogs were anaesthetized with halothane-N2O/O2 and blood was withdrawn until the blood pressure was reduced to 40 mm Hg. This usually required removal of about 30 – 40 % of the total blood volume. The resulting haemorrhagic shock was ascertained by monitoring blood pH, pCO2, pO2, lactate, pyruvate and blood electrolytes. A blood specimen for enzymatic alcohol determination (ADH) was obtained every 30 min over a period of 3 hours. Compared with equally dosed controls the dogs in haemorrhagic shock showed a significant (p = 0.005) reduction of the blood alcohol decay rate (β) which is explained by 1) the diminished blood flow through the liver and 2) the hypoxaemic metabolic situation in shock.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1106
    Keywords: Anterograde transport ; Double labelling ; HRP ; 3H-Proline ; Cat's retinal projections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new method is described for combining 3H-proline and horseradish peroxidase (HRP) as anterograde neuronal tracers. By this method the presence of both substances can be demonstrated in the same histological section. We developed this method to investigate the retinofugal projections from the two eyes in the cat. One eye was injected with 3H-proline the other with HRP. Cryostat sections of the brain were mounted on emulsion coated slides in the dark. Sections were first exposed to the emulsion for 2–3 weeks at −40° C and developed for autoradiography. Only then they were reacted for HRP-activity with tetramethylbenzidine (TMB). Keeping to this sequence autoradiographic procedures could not abolish the HRP-reaction product and silver grains and TMB can be visualized on the same slide. The wellknown projection pattern in the lateral geniculate nuclei was confirmed as a control for the new method. In the superior colliculus and in the pretectum a clear overlap of retinal terminals from the two eyes could be demonstrated for the first time.
    Type of Medium: Electronic Resource
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