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  • 34  (2)
  • Genetic variability  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 32 (1983), S. 75-78 
    ISSN: 1432-0649
    Keywords: 33 ; 34 ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Multiphoton absorption (MPA) of the CO2 laser 9 μmR(30) line (pulse width 60 ns, FWHM) by NH3 and NH3/N2 mixtures was studied as a function of gas composition, pressure and laser fluence (Φ). MPA occurs for Φ smaller than those that have been used for optically pumped NH3/N2 lasers; consequently a simple two-level absorption model will not adequately describe laser action in these systems. The photon energy deposited in NH3/N2 mixtures can be calculated from the MPA cross-section and the fluence dependence of the illumination geometry. An examination of the efficiency of conversion of this absorbed energy to the reported radiant energy of optically pumped NH3/N2 lasers shows an optimum value which depends on pressure, and gas composition.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 38 (1985), S. 51-55 
    ISSN: 1432-0649
    Keywords: 33 ; 34
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Both the multiphoton absorption and the luminescence brightness of osmium tetroxide induced by absorption of temporally smooth CO2 10P(20) laser pulses of 10 and 40 ns FWHM have been measured for pressures in the range 6.7 to 200 Pa. A dependence of these phenomena on laser pulse length is observed. Comparison of the luminescence brightness to the average excitation shows that the distribution of the multiphoton excitation depends on the laser pulse intensity for the two pulse lengths studied.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Nicotiana ; Somatic hybrids ; Protoplast fusion ; Genetic variability ; Variegation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrids have been produced between N. tabacum and two closely related species in the genus Nicotiana, N. otophora and N. sylvestris, to evaluate interclonal variation and genetic behavior of these hybrids. As with the previously reported N. nesophila+ N. tabacum somatic hybrids, we have detected variation for morphological and isoenzyme characters between somatic hybrid clones, despite stability of chromosome number. One clone of N. sylvestris+N. tabacum was marked by variation in leaf spot frequency. The inheritance of this unstable trait was monitored through two sexual generations. Transmission of the Su gene marker was monitored in self-fertilized and back-crossed progeny of the N. sylvestris+N. tabacum somatic hybrids. Segregation ratios were similar to those previously reported for amphiploid N. sylvestris x N. tabacum sexual hybrids.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 62 (1982), S. 193-198 
    ISSN: 1432-2242
    Keywords: Nicotiana ; Somatic hybrids ; Protoplast fusion ; Ovule culture ; Genetic variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrids were produced between Nicotiana tabacum and N. nesophila, two species incapable of conventional sexual hybridization. Sexual hybrids, though, could be produced between these two species by using ovule culture only when N. nesophila was female. Clones of somatic hybrids were compared with sexual hybrids. Statistically significant variation was observed between clones, but not between sexual hybrids, for pollen viability, flower morphology, leaf morphology, and trichome density. As all clones of somatic hybrids have 96 chromosomes, the variability could not be explained by interclonal variation in chromosome number. Variation between somatic hybrids could be the result of cytoplasmic segregation or recombination, mitotic recombination or small chromosomal rearrangements prior to plant regeneration. Variation between clones could be exploited as these interspecies hybrids are now being used to incorporate disease resistance into cultivated tobacco.
    Type of Medium: Electronic Resource
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