ISSN:
0173-0835
Keywords:
Anti-hapten antibodies
;
Fluorescein isothiocyanate
;
Two-dimensional affinity electrophoresis
;
Carrier
;
Epitopic suppression
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Effects of a carrier, bovine serum albumin (BSA) and a hapten array, fluorescein isothiocyanate-conjugated dextran (FITC-DEX), on the production of anti-FITC antibodies in BALB/c mice were analyzed by two-dimensional affinity electrophoresis (2-D AEP). The mice were immunized with FITC-BSA, followed by immunizations with FITC-BSA, saline, BSA, human serum albumin (HSA), fluorescein (Flu), and FITC-DEX. The heterogeneity and quantity of anti-FITC antibodies were increased markedly during the second and third immunization with FITC-BSA. However, the production of anti-FITC antibodies with low affinity to FITC was suppressed by the second and third immunization with the carrier protein of BSA. The suppression of anti-FITC antibodies by immunization with BSA may be due to the major histocompatibility complex (MHC)-mediated competition through antigen processing and the clonal expansion of BSA-specific B cells because similar suppression was induced by the immunization with HSA. The hapten array of FITC-DEX generally suppressed the production of anti-FITC antibodies; however, immunization with free hapten of Flu did not affect the production significantly. This may indicate that cross-linking of anti-FITC antibodies on the surface of specific B cells via the binding of hapten array is required for the suppression.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150190848
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