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  • Immunogold labelling  (2)
  • Knee prostheses  (2)
  • 03. Hydrosphere::03.01. General::03.01.06. Paleoceanography and paleoclimatology  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 278 (1986), S. 283-292 
    ISSN: 1432-069X
    Keywords: Keratinocyte differentiation ; Desmosome ; Immunogold labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The keratinocyte membrane antigen KM 48 was defined by a new monoclonal antibody obtained after mouse immunization with normal human epidermal cell suspension. Specific reactivity of the antibody with desmosomal regions of keratinocyte cell membrane was demonstrated by immunoelectronmicroscopy. Langerhans cells, melanocytes, and indeterminate cells did not express the KM 48 antigen. Immunogold labelling permitted ultrastructural quantitation of KM 48 antibody binding on keratinocytes from various epidermal layers. A gradual increase in desmosome-related KM 48 antigen expression accompanied differentiation of keratinocytes durign their transit from basal to granular layer. Distribution of the antigen on individual cells was uneven. The upper pole of a keratinocyte facing overlying more differentiated cells was always found to be laden with an immunogold marker about twice that of the opposite, lower surface of the cell. The results support the previous reports on gradual development of desmosomes during epidermal cell maturation and open up new possibilities for keratinocyte differentiation studies. They also underline the virtues of the immunogold-labelling method used for cell-surface antigen tracing and quantitation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-069X
    Keywords: HLA class I antigens ; Human epidermis ; Immunogold labelling ; Langerhans cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using an immunogold labelling procedure, we quantified the density of major histocompatibility (MHC) class I antigens on the surface of Langerhans cells (LCs) and keratinocytes of the normal human epidermis. According to ultrastructural features, keratinocytes were divided into three subpopulations: stratum basalis (SBK), stratum spinosum (SSK), and stratum granulosum keratinocytes (SGK), and analyzed separately. For this purpose, three monoclonal antibodies (MCAs) were employed: an anti-HLA A,B,C, an anti-B2-microglobulin (B2-m), and a polymorphic anti-HLA A2 Aw69 MCA. Under electron microscopy, quantitative analysis demonstrated: (a) the presence of a high amount of HLA monomorphic determinants on SBK and SSK and moderate but significant labelling of SGK; (b) the very weak density of MHC class I antigens on the surface of epidermal LCs; (c) the expression, at an identical level, of the HLA heavy chain common determinant (HLA A,B,C), B2-m, and the alloantigen HLA A2 by all epidermal cells (ECs) apart from SGKs and LCs that presented far fewer HLA A2 sites than monomorphic determinants (B2-m and HLA A,B,C); (d) the absence of HLA class I on corneocytes and a moderate labelling of melanocytes. A knowledge of the precise quantitative distribution of HLA class I antigens among various cell subpopulations of the normal human epidermis would be very useful for the study and followup of cutaneous malignancies that are known to lose these molecules as well as for the understanding of immune responses, especially allospecific, that involve the skin.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of orthopaedic surgery & traumatology 3 (1993), S. 93-96 
    ISSN: 1432-1068
    Keywords: Biological implants ; Knee prostheses ; Implants biologiques ; Prothèses de genou
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Dans une note préliminaire, les auteurs exposent leur expérience actuelle, dans le domaine de la recherche expérimentale, de l'ancrage biologique des implants inertes par repousse de travées osseuses dans un effet de surface. Celui-ci est constitué d'un grillage multicouche à forme géométrique en alliage de Titane, Aluminium, Vanadium, créant des porosités régulières ouvertes dont le type 1, selon la topographie définie par Ducheyne, est de 488 µ de côté. Après un rapide exposé de la méthodologie expérimentale, les auteurs présentent les résultats obtenus sur le plan qualitatif par l'étude, après double marquage à la tétracycline, en fluorescence par ultraviolets et par examen en lumière polarisée; cette étude permet d'apprécier la texture du tissu osseux et la différenciation du tissu osseux primaire en tissu osseux secondaire lamellaire plus performant sur le plan biomécanique. Les résultats quantitatifs, en cours d'évaluation, obtenus après coloration au Solochrome-Cyanine R, font appel à l'histomorphométrie osseuse par méthode computérisée. Les premiers résultats permettent de considérer que l'effet de surface étudié autorise une véritable ostéo-intégration selon la définition donnée par Albrektssonn.
    Notes: Abstract In a preliminary note, the authors report their current experience in the field of experimental research, regarding the biological fixation of inert implants by bone ingrowth into porous coatings. The implant is made in a multi-layer lattice with a geometrical form in an alloy of Titanium, Aluminium and Vanadium, creating some regular open porosities (type 1, according to the topography defined by Ducheyne measures 488 µ of width). After briefly presenting the experimental methodology, the authors present the qualitative results: - in a study using Tetracycline double-labelling specimens, U.V. fluorescence, - in a polarized light microscopy-study. They appreciate the bony tissue's texture and the differenciation between primary and secondary bone. The latter shows better biomechanical performances. Quantitative results and dynamic evaluation made by using staining with Solochrome-R-Cyanine specimens are analysed by histomorphometry using a computerised method. The preliminary results show that the surface effect makes rehabitation and osteointegration possible, as defined by Albrektssonn.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European journal of orthopaedic surgery & traumatology 3 (1993), S. 143-146 
    ISSN: 1432-1068
    Keywords: Knee prostheses ; Prothèses de genou
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Les auteurs rapportent une expérience homogène de 10 ans d'une prothèse totale de genou cimentée semi-contrainte en acier inoxydable 316 L coulé et coussin tibial amovible en polyéthylène. Sur 68 patients opérés, 50 ont pu être suivis avec un recul moyen de 6 ans. Les résultats obtenus, selon le score 100 points, sont sensiblement comparables à ceux publiés dans la Littérature pour ce type de prothèse avec 84 % de malades entre 80 et 100 et un indice de satisfaction positif dans 94 % des cas. Toutefois, il est noté sur le plan anatomique une fréquence non négligeable de défauts de positionnement (5 %) et sur le plan fonctionnel, 40 % des cas présentent un sentiment d'insécurité à la descente des escaliers. Cette insécurité est liée principalement à des problèmes techniques en rapport avec un ancillaire très approximatif à l'époque étudiée et qui est maintenant amélioré. Malgré ses résultats relativement moyens, ce type de prothèse, en raison de sa facilité et rapidité de mise en place, doit encore trouver des indications chez des malades de grand âge aux exigences physiques modestes.
    Notes: Abstract The authors report an homogeneous series over ten years of a cemented total prosthesis of the knee, semi-constraint, in a stainless steel, 316 L cast, and a removable tibial cushion in polyethylene. On 68 patients operated on, 50 have been followed up with an average follow-up of 6 years. The results obtained, allowing a score of 100 points, are comparable to those published in the literature for this type of prosthesis, with 84 % of patients between 80 and 100, and a positive index of satisfaction in a 94 % of cases. Nevertheless, from an anatomical point of view, we noticed a non negligible frequency of a defect in position (5%) and from a fonctional point of view, 40% of the patients have a feeling of insecurity in going down steps. This feeling of insecurity is particulary linked to technical problems in relation with the instrumentation, very approximate at the time it was studied, and which is now improved. In spite of its average results, this type of prosthesis, thanks to its ease and speed of placement, must find some indications for elderly people with a modest requirements.
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  • 5
    Publication Date: 2022-06-14
    Description: Thirty years after oxygen isotope records frommicrofossils deposited in ocean sediments confirmed the hypothesis that variations in the Earth’s orbital geometry control the ice ages1, fundamental questions remain over the response of the Antarctic ice sheets to orbital cycles2. Furthermore, an understanding of the behaviour of the marine-based West Antarctic ice sheet (WAIS) during the ‘warmer-than- present’ early-Pliocene epoch (̃5–3Myr ago) is needed to better constrain the possible range of ice-sheet behaviour in the context of future global warming3. Here we present a marine glacial record from the upper 600 m of theAND-1B sediment core recovered from beneath the northwest part of the Ross ice shelf by the ANDRILL programme and demonstrate well-dated, ̃40-kyr cyclic variations in ice-sheet extent linked to cycles in insolation influenced by changes in the Earth’s axial tilt (obliquity) during the Pliocene. Our data provide direct evidence for orbitally induced oscillations in the WAIS, which periodically collapsed, resulting in a switch from grounded ice, or ice shelves, to open waters in the Ross embayment when planetary temperatures were up to ̃3 C warmer than today4 and atmospheric CO2 concentration was as high as ̃400 p.p.m.v. (refs 5, 6). The evidence is consistent with a new ice-sheet/ice-shelf model7 that simulates fluctuations in Antarctic ice volume of up to + 7 m in equivalent sea level associated with the loss of the WAIS and up to +3 m in equivalent sea level from the EastAntarctic ice sheet, in response to ocean-induced melting paced by obliquity.During interglacial times, diatomaceous sediments indicate high surface-water productivity, minimal summer sea ice and air temperatures above freezing, suggesting an additional influence of surface melt8 under conditions of elevated CO2.
    Description: Published
    Description: 322 - 328
    Description: 2.2. Laboratorio di paleomagnetismo
    Description: 3.8. Geofisica per l'ambiente
    Description: 2.3. TTC - Laboratori di chimica e fisica delle rocce
    Description: JCR Journal
    Description: reserved
    Keywords: Antarctica ; paleoclimate ; Pliocene ; stratigraphy ; 02. Cryosphere::02.02. Glaciers::02.02.99. General or miscellaneous ; 03. Hydrosphere::03.01. General::03.01.06. Paleoceanography and paleoclimatology ; 04. Solid Earth::04.04. Geology::04.04.10. Stratigraphy
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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