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  • Biochemistry and Biotechnology  (2)
  • 01140307; AESOPS; Aluminium, particulate; Antarctic Environments Southern Ocean Process Study; Cadmium, dissolved; Cadmium, particulate; Cobalt, dissolved; Cobalt, particulate; Copper, dissolved; Copper, particulate; DEPTH, water; Determination of trace metal concentrations (Johnson et al, 1997); GOFLO; Go-Flo bottles; Iron, dissolved; Iron, particulate; JGOFS; Joint Global Ocean Flux Study; Manganese, dissolved; Manganese, particulate; Nathaniel B. Palmer; NBP9701; NBP9701-01-12; Nickel, dissolved; Nickel, particulate; Southern Ocean; Zinc, dissolved; Zinc, particulate  (1)
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  • 1
    facet.materialart.
    Unknown
    Trace metal concentrations at station NBP97-01-01-12 (2003)
    PANGAEA
    Details
    Publication Date: 2024-02-01
    Keywords: 01140307; AESOPS; Aluminium, particulate; Antarctic Environments Southern Ocean Process Study; Cadmium, dissolved; Cadmium, particulate; Cobalt, dissolved; Cobalt, particulate; Copper, dissolved; Copper, particulate; DEPTH, water; Determination of trace metal concentrations (Johnson et al, 1997); GOFLO; Go-Flo bottles; Iron, dissolved; Iron, particulate; JGOFS; Joint Global Ocean Flux Study; Manganese, dissolved; Manganese, particulate; Nathaniel B. Palmer; NBP9701; NBP9701-01-12; Nickel, dissolved; Nickel, particulate; Southern Ocean; Zinc, dissolved; Zinc, particulate
    Type: Dataset
    Format: text/tab-separated-values, 45 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 2
    Electronic Resource
    Electronic Resource
    Investigation of drug metabolism using capillary electrophoresis with photodiode array detection and online mass spectrometry equipped with an array detector (1994)
    Weinheim : Wiley-Blackwell
    Electrophoresis 15 (1994), S. 62-71 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The application of capillary electrophoresis (CE) with photodiode array detection (DAD) and on-line CE-mass spectrometry (CE-MS) equipped with a position and time resolved (PATRICtm) focal plane detector for analysis of both in vitro and in vivo drug metabolism is demonstrated. Separation of metabolites derived from the neuroleptic drug haloperidol, by CE, using a simple, volatile run buffer containing 50 mM ammonium acetate with 10% methanol and 1% acetic acid is reported. The potential utility of CE-DAD for screening drug metabolite mixtures derived from hepatic microsomal incubations is demonstrated for haloperidal (HAL). Also the potential problems associated with using this technology to screen human urine samples for HAL metabolites is discussed. Furthermore, the usefulness of CE-MS and CE-electrospray ionization skimmer collision induced dissociation-MS (CE-ESI-CID-MS) in identification and structure elucidation of HAL metabolites derived from both a guinea pig hepatic microsomal incubation and urine from a patient treated with 0.5 mg/day of HAL is shown. The utility of such an approach in the general area of clinical pharmacology is also discussed.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150150110
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Enhanced sensitivity for sequence determination of major histocompatibility complex class I peptides by membrane preconcentration - capillary electrophoresis -microspray - tandem mass spectrometry (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 2207-2212 
    Details
    ISSN: 0173-0835
    Keywords: On-line preconcentration ; Capillary electrophoresis ; Transient isotachophoresis ; Polybrene ; Microspray mass spectrometry ; Peptide sequencing ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Sequence analysis of antigenic major histocompatibility complex (MHC) class I peptides requires minimizing sample loss and enhancing mass spectrometric sensitivity. In order to facilitate such analyses, we have coupled on-line membrane preconcentration-capillary electrophoresis (mPC-CE) with microspray mass spectrometry (mPC-CE-μMS) and tandem mass spectrometry (mPC-CE-μMS/MS). Specifically, cell lysate from ∼ 109 EG-7 mouse tumor cells was immunoprecipitated and the released MHC class I peptides were subjected to reverse-phase HPLC. An HPLC fraction containing antigenic peptide(s) shown to induce T-cell stimulation was subjected to mPC-CE-μMS. Approximately 10 μL (from 100 μL) of the fraction was pressure-injected and concentrated on a styrenedivinylbenzene (SDB) impregnated membrane. The peptides were eluted from the membrane with ∼100 nL of 80% methanol, sandwiched between a leading stcking buffer (LSB, also serving as CE separation medium) of ∼110 nL of 0.1% acetic acid in 10% methanol, and a trailing stacking buffer (TSB) of ∼ 110 nL of 0.1% NH4OH. On application of the CE voltage the peptides are subjected to moving boundary transient isotachophoresis and focused. The peptides were separated in a Polybrene-coated capillary with application of -20 kV in reverse polarity mode and subsequently sprayed via an emitter coupled to the CE capillary by a liquid junction containing a platinum wire. An ion at m/z 482.3 was detected and subjected to mPC-CE-μMS/MS and determined to be SIINFEKL, a peptide (OVA) known to be antigenic in the mouse model system. Sensitivity enhancement over conventional mPC-CE-MS and MS/MS was ∼100-fold.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191227
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    Paper (German National Licenses)
    Fulltext
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