In:
Journal of Separation Science, Wiley, Vol. 36, No. 12 ( 2013-06), p. 1980-1985
Abstract:
A method of simultaneous separation of water‐ and fat‐soluble vitamins using pressure‐assisted CEC with a methacrylate‐based capillary monolithic column was developed. In the proposed method, water‐soluble vitamins were mainly separated electrophoretically, while fat soluble‐ones were separated chromatographically by the interaction with a methacrylate‐based monolith. A mixture of six water‐soluble and four fat‐soluble vitamins was separated simultaneously within 20 min with an isocratic elution using 1 M formic acid (pH 1.9)/acetonitrile (30:70, v/v) containing 10 mM ammonium formate as a mobile phase. When the method was applied to a commercial multivitamin tablet and a spiked one, the vitamins were successfully analyzed, and no influence of the matrix contained in the tablet was observed.
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.201201191
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2047990-6
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