In:
Journal of Chromatographic Science, Oxford University Press (OUP), Vol. 62, No. 6 ( 2024-07-10), p. 562-569
Abstract:
Fructus Corni (F. Corni) is the dried mature pulp of Cornus officinalis Sieb. et Zucc.(Cornaceae), which is rich in iridoids. In this study, a simple, sensitive and rapid UPLC–MS/MS method was developed for the simultaneous determination of 13 iridoid glycosides of F. Corni from different areas. Specifically, we included five new compounds (cornusdiridoid C, cornusdiridoid E, cornusdiridoid F, 3′′,5′′-dehydroxycornuside and 2′-O-p-coumaroyl-kingiside) and isomers (2′-O-p-E-coumaroylloganin and 2′-O-p-Z-coumaroylloganin) for the first time in the quality markers of F. Corni. A total of 13 compounds and two pairs of isomers were well isolated and tested within just 14 min. All calibration curves showed good linear regression (r2 ≥ 0.99) within the tested concentration ranges. The limit of detection and limit of quantification were in the range of 0.19–1.90 and 0.38–3.76 ng/mL, respectively. The intra-day and inter-day precision were & lt;3.21% and 12.49%, the RSD values of repeatability did not exceed 6.81% and the average recoveries were 90.95–113.59% for the analytes. All iridoid glycosides stabilized within 12 h (RSD & lt; 10.99%). This method has been successfully applied to the quality evaluation of crude and processed F. Corni from different areas. The determination of characteristic iridoid glycosides and isomers will provide a more reliable and comprehensive method for the evaluation of F. Corni.
Type of Medium:
Online Resource
ISSN:
0021-9665
,
1945-239X
DOI:
10.1093/chromsci/bmad083
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2024
detail.hit.zdb_id:
80141-0
detail.hit.zdb_id:
2044085-6
Permalink