In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-12-12)
Abstract:
Homologous recombination involving sister chromatids is the most accurate, and thus most frequently used, form of recombination-mediated DNA repair. Despite its importance, sister chromatid recombination is not easily studied because it does not result in a change in DNA sequence, making recombination between sister chromatids difficult to detect. We have previously developed a novel DNA template strand sequencing technique, called Strand-seq, that can be used to map sister chromatid exchange (SCE) events genome-wide in single cells. An increase in the rate of SCE is an indicator of elevated recombination activity and of genome instability, which is a hallmark of cancer. In this study, we have adapted Strand-seq to detect SCE in the yeast Saccharomyces cerevisiae. We provide the first quantifiable evidence that most spontaneous SCE events in wild-type cells are not due to the repair of DNA double-strand breaks.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.30560.001
DOI:
10.7554/eLife.30560.002
DOI:
10.7554/eLife.30560.003
DOI:
10.7554/eLife.30560.004
DOI:
10.7554/eLife.30560.005
DOI:
10.7554/eLife.30560.006
DOI:
10.7554/eLife.30560.007
DOI:
10.7554/eLife.30560.008
DOI:
10.7554/eLife.30560.009
DOI:
10.7554/eLife.30560.010
DOI:
10.7554/eLife.30560.011
DOI:
10.7554/eLife.30560.012
DOI:
10.7554/eLife.30560.014
DOI:
10.7554/eLife.30560.015
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
Permalink