In:
eLife, eLife Sciences Publications, Ltd, Vol. 5 ( 2016-11-28)
Abstract:
The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymena’s germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.19090.001
DOI:
10.7554/eLife.19090.002
DOI:
10.7554/eLife.19090.003
DOI:
10.7554/eLife.19090.004
DOI:
10.7554/eLife.19090.005
DOI:
10.7554/eLife.19090.006
DOI:
10.7554/eLife.19090.007
DOI:
10.7554/eLife.19090.008
DOI:
10.7554/eLife.19090.009
DOI:
10.7554/eLife.19090.010
DOI:
10.7554/eLife.19090.011
DOI:
10.7554/eLife.19090.012
DOI:
10.7554/eLife.19090.013
DOI:
10.7554/eLife.19090.014
DOI:
10.7554/eLife.19090.015
DOI:
10.7554/eLife.19090.016
DOI:
10.7554/eLife.19090.017
DOI:
10.7554/eLife.19090.018
DOI:
10.7554/eLife.19090.019
DOI:
10.7554/eLife.19090.020
DOI:
10.7554/eLife.19090.021
DOI:
10.7554/eLife.19090.022
DOI:
10.7554/eLife.19090.023
DOI:
10.7554/eLife.19090.024
DOI:
10.7554/eLife.19090.025
DOI:
10.7554/eLife.19090.026
DOI:
10.7554/eLife.19090.027
DOI:
10.7554/eLife.19090.028
DOI:
10.7554/eLife.19090.029
DOI:
10.7554/eLife.19090.030
DOI:
10.7554/eLife.19090.031
DOI:
10.7554/eLife.19090.032
DOI:
10.7554/eLife.19090.033
DOI:
10.7554/eLife.19090.034
DOI:
10.7554/eLife.19090.035
DOI:
10.7554/eLife.19090.044
DOI:
10.7554/eLife.19090.045
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2016
detail.hit.zdb_id:
2687154-3
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