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  • World Scientific Pub Co Pte Ltd  (93)
  • 1
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2023
    In:  The American Journal of Chinese Medicine Vol. 51, No. 06 ( 2023-01), p. 1577-1594
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 51, No. 06 ( 2023-01), p. 1577-1594
    Abstract: Colorectal cancer (CRC) is a leading cause of cancer-related death in the United States, and chronic gut inflammation is a risk factor for CRC initiation and development. Curcuma longa L., or turmeric, has become one of the most studied herbal medicines in recent years due to its anticancer potentials. It is generally accepted that the major component in turmeric is curcuminoids, and the active constituent in curcuminoids is curcumin. However, unprocessed curcumin is characterized by poor water solubility, which means low bioavailability in humans. To increase the bioavailability of curcumin, in this study, we utilized a novel surfactant-formulated curcumin (CuminUP60[Formula: see text]) and evaluated its CRC chemopreventive activities. Compared with the chemo-sensitive CRC cell line HCT-116, the management of the CRC SW-480 cell line is a challenge, since the latter is chemo-resistant. In other words, these cancer cells resist the effects of the chemotherapy. Using the newly formulated CuminUP60[Formula: see text] water solution, this study demonstrated its strong antiproliferative effects on the SW-480 cells in a dose- and time-dependent manner. This new formulation induced early apoptosis and arrested the cell cycle in the G2/M phase via the upregulation of cyclin B1. We also observed that this new formulation possessed inhibitory effects on Th17 cell differentiation, which regulates the body’s immune response against gut malignancies. In summary, our results exhibited a potential clinical utility of the surfactant-formulated curcumin in chemo-resistant colorectal cancer management.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2023
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  • 2
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2017
    In:  The American Journal of Chinese Medicine Vol. 45, No. 01 ( 2017-01), p. 13-22
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 45, No. 01 ( 2017-01), p. 13-22
    Abstract: Angiogenesis is a regulated process integral to many physiological and pathological situations, including carcinogenesis and tumor growth. The majority of the angiogenic processes are related to inflammation. The interplay is not only important in the case of pathogen entry but also influential in chronic inflammatory diseases, tumor growth and tissue regeneration. Modulating the interaction between inflammation and angiogenesis could be an important target for cancer treatment and wound healing alike. Ginseng has a wide range of pharmacological effects, including anti-inflammatory and angiogenesis-modulating activities. This paper presents the recent research progresses on the inhibition of angiogenesis by ginseng and its active constituents, with a particular focus on processes mediated by inflammation. The modulatory role of ginseng compounds in inflammation-mediated angiogenesis involving hypoxia and microRNAs are also discussed. With the potential to modulate the angiogenesis at the transcriptional, translational and protein signaling level via various different mechanisms, ginseng could prove to be effective in cancer therapeutics.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2017
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  • 3
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2024
    In:  The American Journal of Chinese Medicine Vol. 52, No. 01 ( 2024-01), p. 35-55
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 52, No. 01 ( 2024-01), p. 35-55
    Abstract: Asian ginseng, the root of Panax ginseng C.A. Meyer, occupies a prominent position in the list of best-selling natural products in the world. There are two major types of ginseng roots: white ginseng and red ginseng, each with numerous preparations. White ginseng is prepared by air-drying fresh Asian ginseng roots after harvest. Red ginseng is prepared by steaming roots in controlled conditions using fresh or raw Asian ginseng. Red ginseng is commonly used in Asian countries due to its unique chemical profile, different therapeutic efficacy, and increased stability. Compared with the widespread research on white ginseng, the study of red ginseng is relatively limited. In this paper, after a botanical feature description, the structures of different types of constituents in red ginseng are systematically described, including naturally occurring compounds and those resulting from the steam processing. In red ginseng phytochemical studies, the number of published reports on ginsenosides is significantly higher than that for other constituents. Up to now, 57 ginsenosides have been isolated and characterized in red ginseng. The structural transformation pathways during steaming have been summarized. In comparison with white ginseng, red ginseng also contains other constituents, including polyacetylenes, Maillard reaction products, other types of glycosides, lignans, amino acids, fatty acids, and polysaccharides, which have also been presented. Appropriate analytical methods are necessary for differentiating between unprocessed white ginseng and processed red ginseng. Specific marker compounds and chemical profiles have been used to discriminate red ginseng from white ginseng and adulterated commercial products. Additionally, a brief phytochemical profile comparison has been made between white ginseng and black ginseng, and the latter is another type of processed ginseng prepared from white or red ginseng by steaming several times. In conclusion, to ensure the safe and effective use of red ginseng, phytochemical and analytical studies of its constituents are necessary and even crucial.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2024
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  • 4
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2015
    In:  The American Journal of Chinese Medicine Vol. 43, No. 04 ( 2015-01), p. 743-756
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 43, No. 04 ( 2015-01), p. 743-756
    Abstract: Chemopreventive agents can be identified from botanicals. Recently, there has been strong support for the potential of 6-shogaol, a natural compound from dietary ginger (Zingiber officinale), in cancer chemoprevention. However, whether 6-shogaol inhibits the growth of colorectal tumors in vivo remains unknown, and the underlying anticancer mechanisms have not been well characterized. In this work, we observed that 6-shogaol (15 mg/kg) significantly inhibited colorectal tumor growth in a xenograft mouse model. We show that 6-shogaol inhibited HCT-116 and SW-480 cell proliferation with IC50 of 7.5 and 10 μM, respectively. Growth of HCT-116 cells was arrested at the G2/M phase of the cell cycle, primarily mediated by the up-regulation of p53, the CDK inhibitor p21 waf1/cip1 and GADD45α, and by the down-regulation of cdc2 and cdc25A. Using p53 -/- and p53 +/+ HCT-116 cells, we confirmed that p53/p21 was the main pathway that contributed to the G2/M cell cycle arrest by 6-shogaol. 6-Shogaol induced apoptosis, mainly through the mitochondrial pathway, and the bcl-2 family might act as a key regulator. Our results demonstrated that 6-shogaol induces cancer cell death by inducing G2/M cell cycle arrest and apoptosis. 6-Shogaol could be an active natural product in colon cancer chemoprevention.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2015
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  • 5
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2015
    In:  The American Journal of Chinese Medicine Vol. 43, No. 06 ( 2015-01), p. 1231-1246
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 43, No. 06 ( 2015-01), p. 1231-1246
    Abstract: To improve the transdermal delivery of ligustrazine, Foeniculum vulgare food origin anisole compounds were employed as promoters. Transdermal fluxes of ligustrazine were determined by Franz-type diffusion cells. Fourier transform-infrared (FT-IR) spectra were used to detect the biophysical changes of the stratum corneum and to explore the mechanism of permeation enhancement. A scanning electron microscope (SEM) was used to monitor the morphological changes of the skin. Among the three anisoles, anisic acid increased the penetration flux of ligustrazine significantly. The ligustrazine flux with anisic acid (11.9 μg/cm 2 /h) was higher than that any other group (p 〈 0.05). Spectra observations revealed that these anisole enhancers were able to disturb and extract the stratum corneum lipids. In addition, apparent density was used to describe the desquamation extent of the scutella. Multiple mechanisms are involved in the permeation enhancement of ligustrazine, including disturbing and extracting stratum corneum lipid, forming a competitive hydrogen bond. All data suggested that anisole compounds could be a group of safe and active penetration enhancers for transdermal delivery of ligustrazine.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2015
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  • 6
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2014
    In:  The American Journal of Chinese Medicine Vol. 42, No. 03 ( 2014-01), p. 729-742
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 42, No. 03 ( 2014-01), p. 729-742
    Abstract: Bufalin is a key component of a Chinese medicine (Chan Su) and has been proved effective in killing various cancer cells. Its role in inducing DNA damage and the inhibition of the DNA damage response (DDR) has been reported, but none have studied such action in lung cancer in detail. In this study, we demonstrated bufalin-induced DNA damage and condensation in NCI-H460 cells through a comet assay and DAPI staining, respectively. Western blotting indicated that bufalin suppressed the protein levels associated with DNA damage and repair, such as a DNA dependent serine/threonine protein kinase (DNA-PK), DNA repair proteins breast cancer 1, early onset (BRCA1), 14-3-3 σ (an important checkpoint keeper of DDR), mediator of DNA damage checkpoint 1 (MDC1), O6-methylguanine-DNA methyltransferase (MGMT) and p53 (tumor suppressor protein). Bufalin could activate phosphorylated p53 in NCI-H460 cells. DNA damage in NCI-H460 cells after treatment with bufalin up-regulated its ATM and ATR genes, which encode proteins functioning as sensors in DDR, and also up-regulated the gene expression (mRNA) of BRCA1 and DNA-PK. But bufalin suppressed the gene expression (mRNA) of p53 and 14-3-3 σ, however, bufalin did not significantly affect the mRNA of MGMT. In conclusion, bufalin induced DNA damage in NCI-H460 cells and also inhibited its DNA repair and checkpoint function.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2014
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  • 7
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 43, No. 03 ( 2015-01), p. 581-600
    Abstract: Bladder cancer is a common malignancy worldwide. However, there is still no effective therapy for bladder cancer. In this study, we investigated the cytotoxic effects of cantharidin [a natural toxin produced (pure compound) from Chinese blister beetles (Mylabrisphalerata or Mylabriscichorii) and Spanish flies (Cantharis vesicatoria)] in human bladder cancer cell lines (including: T24 and RT4 cells). Treatment of human bladder cancer cells with cantharidin significantly decreased cell viability. The increase in the expressions of caspase-3 activity and cleaved form of caspase-9/-7/-3 were also increased in cantharidin-treated T24 cells. Furthermore, cantharidin increased the levels of phospho-eIF2α and Grp78 and decreased the protein expression of procaspase-12, which was accompanied by the increase in calpain activity in T24 cells. Cantharidin was capable of increasing the intracellular Ca 2+ and the phosphorylation of protein kinase C (PKC) in T24 cells. The addition of BAPTA/AM (a Ca 2+ chelator) and RO320432 (a selective cell-permeable PKC inhibitor) effectively reversed the increase in caspase-3 and calpain activity, the phosphorylation levels of PKC and eIF2α and Grp78 protein expression, and the decrease in procaspase-12 expression induced by cantharidin. Importantly, cantharidin significantly decreased the tumor volume (a dramatic 71% reduction after 21 days of treatment) in nude mice xenografted with T24 cells. Taken together, these results indicate cantharidin induced human bladder cancer cell apoptosis through a calcium/PKC-regulated ER stress pathway. These findings suggest that cantharidin may be a novel and potential anticancer agent targeting on bladder cancer cells.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2015
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  • 8
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 45, No. 06 ( 2017-01), p. 1293-1307
    Abstract: The root of Asian ginseng (Panax ginseng C.A. Meyer) has been used for centuries in Oriental medicine to improve general well-being and to relieve various medical conditions. It is commonly understood that ginsenosides are responsible for the pharmacological activities of ginseng. Compared to the root of ginseng, studies on the berry are considerably limited. In this study, we evaluated the effects of polysaccharides from Asian ginseng berries on plasma lipid levels, chemically-induced enteric inflammation and neoplasm, and cancer chemoprevention in different experimental models. We tested two polysaccharide preparations: regular ginseng berry polysaccharide extract (GBPE) and ginseng berry polysaccharide portion (GBPP, removed MV [Formula: see text]). We first observed that both oral GBPE and oral GBPP significantly reduced plasma cholesterol and triglycerides levels in a dose-related manner in ob/ob mice, without obvious body weight changes. Then, in AOM/DSS-induced acute colitis mice, GBPE and GBPP significantly ameliorated the increased gut disease activity index and inhibited the reduction of the colon length. Further, the berry polysaccharides significantly suppressed chemically-induced pro-inflammatory cytokine levels. This is consistent with the observation that GBPE and GBPP attenuated tumorigenesis in mice by significantly and dose-dependently reducing tumor load. Finally, in vitro HCT-116 and HT-29 human colon cancer cells were used. While these berry preparations had better antiproliferation effects on the HCT-116 than the HT-29 cells, the GBPE had significantly stronger inhibitory effects than GBPP. The observed in vitro GBPE’s effect could contribute to the actions of its small-molecule non-polysaccharide compounds due to their direct antiproliferative activities. Results obtained from the present study suggest that ginseng berry polysaccharides may have a therapeutic role in the management of high lipid levels, enteric inflammation, and colon malignancies.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2017
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  • 9
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2022
    In:  The American Journal of Chinese Medicine Vol. 50, No. 06 ( 2022-01), p. 1663-1679
    In: The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 50, No. 06 ( 2022-01), p. 1663-1679
    Abstract: Gambogic acid (GA), a natural and bioactive compound from the gamboge resin, has been reported to exhibit many oncostatic activities against several types of malignancies. However, its effects on the progression of oral squamous cell carcinoma (OSCC) remain largely unexplored. To fill this gap, we investigated the anticancer role of GA and molecular mechanisms underlying GA’s actions in combating oral cancer. We found that GA negatively regulated the viability of OSCC cells, involving induction of the sub-G1 phase and cell apoptosis. In addition, a specific signature of apoptotic proteome, such as upregulation of heme oxygenase-1 (HO-1) and activation of caspase cascades, was identified in GA-treated OSCC. Moreover, such induction of HO-1 expression and caspase cleavage by GA was significantly diminished through the pharmacological inhibition of p38 kinase. In conclusion, these results demonstrate that GA promotes cell apoptosis in OSCC, accompanied with the activation of a p38-dependent apoptotic pathway. Our findings provide potential avenues for the use of GA with high safety and therapeutic implications in restraining oral cancer.
    Type of Medium: Online Resource
    ISSN: 0192-415X , 1793-6853
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2022
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  • 10
    Online Resource
    Online Resource
    World Scientific Pub Co Pte Ltd ; 2014
    In:  Journal of Mechanics in Medicine and Biology Vol. 14, No. 01 ( 2014-02), p. 1450010-
    In: Journal of Mechanics in Medicine and Biology, World Scientific Pub Co Pte Ltd, Vol. 14, No. 01 ( 2014-02), p. 1450010-
    Abstract: Palpation is an essential skill of manual therapy. Clinical techniques of physical therapy usually assume that the movement direction of palpating spinous process (SP) is the direction of the vertebral body center (VBC). This study investigated the distance [SP–projected VBC (PVBC)] between the surface palpation of the five SPs and the radiographically projected vertebral center locations (PVBC) on the skin of the lumbar spine in 37 patients with low back pain (LBP). The measurement of SP–PVBC was intended to describe if palpation on SPs could explain the positions of the VBC. The SP–PVBC distance was the greatest at L1 (35.9 mm) and the smallest at L4 (15.1 mm). The predictive analysis investigated the relationships between SP–PVBC and the geometric measurements of the lumbar anatomical structures. The geometric characteristics of the lumbar spine affected the SP–PVBC distance in different levels, with the R 2 values from 0.66–0.89, except 0.38 in the L4 level. Increases in the SP inclination as well as vertebral inclinations, and increases in the SP height (SPH) were factors that were found to be significantly related to the SP–PVBC distance (p 〈 0.05). The results indicate that the orientation of the VBC and the SP may not be the same, and tilting and rotation of the vertebrae may occur when applying manual techniques through SPs. Physical therapists need to be aware that the tilting or rolling effect of vertebrae may not be avoidable once the treatment is done via palpation on SPs.
    Type of Medium: Online Resource
    ISSN: 0219-5194 , 1793-6810
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2014
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