In:
Functional Materials Letters, World Scientific Pub Co Pte Ltd, Vol. 11, No. 05 ( 2018-10), p. 1850046-
Abstract:
Constant increase of greenhouse gas emission by human activity causes a climate change, with carbon dioxide as the main contributor. In nature, CO 2 fixation takes place in leaves where carbonic anhydrase (CA) catalyzes the hydration reaction. Inspired by this photosynthesis process, we come with a polysulfone biomimetic membrane containing CA for CO 2 capture. Two immobilization approaches were investigated: physical and chemical. For this reason, we prepared a blank polysulfone membrane to physically adsorb the enzyme on its surface, and a membrane with dispersed ferritic nanoparticles -NH 2 terminated used for covalent binding of the enzyme. The scope of this study is to evaluate the influence of the immobilization type on the enzyme activity. The obtained nanoparticles and membranes were characterized by TEM, WAXD, EDX, SEM and CA, respectively. The attached amount was determined by ICP, whereas the activity and CO 2 solubility were estimated experimentally. Studies showed that the enzyme is attached more efficiently by the physical adsorption. Nevertheless, covalent binding favors its activity by stabilizing the structure.
Type of Medium:
Online Resource
ISSN:
1793-6047
,
1793-7213
DOI:
10.1142/S1793604718500467
Language:
English
Publisher:
World Scientific Pub Co Pte Ltd
Publication Date:
2018
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