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  • 1
    Electronic Resource
    Electronic Resource
    Sequencing of oligonucleotides using high performance liquid chromatography and electrospray mass spectrometry (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 897-901 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES-MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off-line high performance liquid chromatography was used. For ES-MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium-adduct ion peaks.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091007
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  • 2
    Electronic Resource
    Electronic Resource
    Increased vascularity of the lingual salt glands of the estuarine crocodile, Crocodylus porosus, kept in hyperosmotic salinity (1993)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 218 (1993), S. 143-151 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Methyl methacrylate corrosion casts were made of the blood-vascular system of the lingual salt glands of the estuarine crocodile, Crocodylus porosus, and examined with light and scanning electron microscopy. The 28-40 individual salt glands, each opening separately via a single pore onto the dorsal surface of the tongue, are supplied by a pair of lingual arteries. Each gland is richly vascularized and is composed of 14-20 lobular sub-units, each having a dense network of capillaries. The blood flow in each gland is from the centre to its periphery, opposite to the direction of the flow of secretions in the ducts of the gland. The main collecting duct leading from the gland to the external pore was well vascularized. The blood supply to the glands of juvenile crocodiles raised in 20‰ salt water was more dense than in freshwater and, from cast masses, had a three-fold greater vascular volume. This study provides the first evidence which shows that the salt glands of crocodiles are morphologically labile and can adapt to the environmental salinity. © 1993 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052180204
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of age of the donor and recipient on the development of transferred golden hamster ovaBased upon a portion of a thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Anatomy in the University of Illinois at the Medical Center.Research supported by a grant from the University of Illinois Foundation. (1964)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 150 (1964), S. 413-416 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ova were removed from donors 63-68 hours after ovulation and placed in the uteri of pseudopregnant recipients of the same pregnancy age. In transfers of ova from young (2 1/2-6 month) to old (14-18 month) golden hamsters, only 8.3% developed into term fetuses. From old donors to young recipients, 4.5% of ova developed to term fetuses. In transfers of ova from young donors to young recipients, 49.2% of ova developed into term fetuses. There was no significant difference in percentage of fetal development between the two groups containing old females. The difference between these and the control (young to young) group was highly significant.There was a significantly lower number of ova recovered from old donors. At time of transfer, over one-sixth of the ova from old donors consisted of only one to four cells. At seven and one-half days, embryos developing from ova of old donors in uteri of young recipients were retarded and malformed.It is concluded that while the uterine environment in old hamsters appears inadequate to foster normal fetal development, there are also fewer ova ovulated in old females and these ova more frequently develop abnormally.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091500409
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  • 4
    Electronic Resource
    Electronic Resource
    Reproductive senescence in the female golden hamsterBased upon a portion of a thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Anatomy in the University of Illinois at the Medical Center.Research supported by a grant from the University of Illinois Foundation. (1964)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 150 (1964), S. 405-411 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The first statistically significant decline in the mean number of young born to female golden hamsters occurred in the fourth litters. The decreased size of fifth litters was highly significant and few females bore six litters.Hamsters unilaterally ovariectomized at 30 days of age had a significantly lower number of litters containing live young during their lifetime. Despite increased unilateral ovulaton, they bore less than half as many total young per female as intact controls and fetal resorptions and stillbirths occurred at an earlier age.The results of matings during the period of reproductive decline were observed in females from the age of ten months until death or sacrifice at an advanced age. Final litters contained few young, some born dead. One or two subsequent pregnancies were of term length but all conceptuses were resorbed. Few females beyond 15 months of age had implantation sites and most of these resorbed. Regular four day estrous cycles continued until a terminal illness and matings resulted in pseudopregnancy. Blastocysts were found at four and one-half days of gestation in some old females but not at 6 to 8 days. Failure of implantation appeared to be due to an inadequate uterine decidual reaction in the old animals.It appeared from the above observations that decreasing uterine adaptability limited reproductive capacity in aging hamsters.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091500408
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  • 5
    Electronic Resource
    Electronic Resource
    Structural analogies for scalar field problems (1981)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 17 (1981), S. 471-476 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper describes how general-purpose finite element structural analysis computer programs can be used, without modification, to solve various scalar field equations such as the wave equation, the Helmholtz equation, Laplace's equation, Poisson's equation, the heat equation and the telegraph equation, as well as mixed field problems (such as coupled structural-acoustic problems) which involve these equations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620170313
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  • 6
    Electronic Resource
    Electronic Resource
    A comparasion of three resequencing algorithms for the reduction of matrix profile and wavefront (1979)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 14 (1979), S. 837-853 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Three widely-used nodal resequencing algorithms were tested and compared for their ability to reduce matrix profile and root-mean-square (rms) wavefront, the latter being the most critical parameter in determining matrix decomposition time in the NASTRAN finite element computer program. The three algorithms are Cuthill-McKee (CM), Gibbs-Poole-Stockmeyer (GPS), and Levy. Results are presented for a diversified collection of 30 test problems ranging in size from 59 to 2680 nodes. It is concluded that GPS is exceptionally fast, and, for the conditions under which the test was made, the algorithm best able to reduce profile and rms wavefront consistently well. An extensive bibliography of resequencing algorithms is included.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620140606
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  • 7
    Electronic Resource
    Electronic Resource
    How do the migratory and adhesive properties of the neural crest govern ganglia formation in the avian peripheral nervous system? (1985)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 27 (1985), S. 189-203 
    Details
    ISSN: 0730-2312
    Keywords: avian embryo ; peripheral nervous system ; neural crest ; cell-adhesion ; cell migration ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The peripheral nervous system derives mainly from the neural crest both in the head and trunk. Using markers such as fibronectin (FN), neural cell-adhesion molecule (NCAM), the nuclcolar marker for quail cells in chimaeric embryos, and NC-1, a monoclonal antibody specific to crest cells and their neural derivatives, we have attempted to reconstruct the processes that lead to the formation of peripheral ganglia. Our observations allow us to propose a model of the formation of ganglia based on morphogenetic movements and on variations of crest cell adhesiveness. In most cases, crest cells migrate in morphologically defined and transient pathways that lead them to their final site of arrest; these pathways are always associated with FN, which appears necessary for crest cell attachment and movement in vitro. The directionality of crest cell migration is probably dictated by the cells' motile properties and population pressure in restricted areas suitable for cell movement. The disappearance of the pathways and of the substrate necessary for migration while the population is rapidly dividing may be responsible for the aggregation of crest cells in the case of the sensory ganglia. To the contrary, the aggregation of crest cells into autonomic ganglia (sympathetic, enteric, aid ciliary ganglia) does not seem to obey the same rules, no disappearance of the substratum or of the pathways being obvious; rather, their formation seems correlated with the de novo synthesis of adhesive molecules such as NCAM.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240270302
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  • 8
    Electronic Resource
    Electronic Resource
    Uptake of 125I-labelled α2-macroglobulin and albumin by human placental syncytiotrophoblast in vitro (1998)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 68 (1998), S. 427-435 
    Details
    ISSN: 0730-2312
    Keywords: α2-macroglobulin ; albumin ; placenta ; zinc ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We have investigated the binding and internalization of α2-macroglobulin and serum albumin by human placental syncytiotrophoblast cells in vitro. The time course (obtained at 4°C) of α2-macroglobulin binding indicated that an equilibrium was reached after 4 h. The binding of 125I-labelled α2-macroglobulin to syncytiotrophoblast cells was competitively reduced in the presence of excess unlabelled α2-macroglobulin. When the concentration-dependence of binding was examined over a wide concentration range, non-linear regression analysis yielded a Kd of 6.4 nM. In the case of albumin, binding was weak and ligand dissociated from the cell surface during aqueous washing making it impractical to analyze the binding reaction. In other experiments, syncytiotrophoblast cells were incubated with 125I-labelled α2-macroglobulin at 37°C. Under these conditions, trypsin-resistant cell-associated radioactivity increased with time consistent with ligand internalization. 125I-Labelled-ligand was internalized with a t1/2 of about 5 min. After a lag period some radioactivity was released back into the incubation medium. When measured at times up to 210 min, this was found to consist of mostly TCA-precipitable material that had been lost from the cell surface. However, when the incubation was extended to 24 h, almost 15% of the initial cell-associated radioactivity was released to the extracellular medium as TCA-soluble material, consistent with a slow rate of ligand degradation. The specific binding of 65Zn-labelled α2M was similar to that of the 125I-labelled ligand and trypsin-resistance measurements provided evidence of α2M-mediated 65Zn uptake. These results support a role for syncytiotrophoblast in the metabolism of α2-macroglobulin during pregnancy and are also consistent with a role for α2-macroglobulin in the maternal-fetal transport of zinc. J. Cell. Biochem. 68:427-435, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Differentiation of human trophoblast cells in vitro is inhibited by dimethylsulfoxide (1997)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 65 (1997), S. 460-468 
    Details
    ISSN: 0730-2312
    Keywords: placenta ; planar-polar compounds ; hCG ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Dimethyl sulfoxide (DMSO) exerts a number of biological effects, the most frequently cited being induction of cell differentiation. The compound also increases invasiveness and metastatic potential. In contrast to the many reports of DMSO-induced cell differentiation, we report here that DMSO inhibits the morphological differentiation of human cytotrophoblast cells to syncytiotrophoblast, as revealed by immunofluorescence staining for desmosomal protein and nuclei. Cytotrophoblast cells treated with DMSO under differentiation-inducing conditions remained mononucleated with intense desmosomal staining. The effect was dose dependent, with a maximal effect seen at 1.5% DMSO. Concentrations of ≤0.5% had no effect and concentrations 〉2% were cytotoxic. In addition to these morphological changes, DMSO inhibited secretion of human chorionic gonadotropin in a dose-dependent manner. At a concentration of 1.5%, DMSO inhibited secretion by 70%. If cytotrophoblast cells were cultured in the presence of DMSO and then switched to DMSO-free medium, they proceeded to differentiate normally. While the precise mechanism of action remains unknown, judicious use of DMSO may be a useful tool for studying and manipulating the differentiation of human trophoblast cells in vitro. The findings also indicate that care should be used in interpreting results obtained using DMSO as a carrier in drug and inhibitor studies. J. Cell Biochem. 65:460-468. © 1997 Wiley-Liss Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Influence of surfactants on the conformation of β-lactoglobulin B using circular dichroismPresented in part at the 1981 Joint Meeting of the Central and Great Lakes ACS Regions of the American Chemical Society, Dayton, Ohio, May 20-22, 1981. (1982)
    New York : Wiley-Blackwell
    Biopolymers 21 (1982), S. 895-908 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The effect of several surfactants on the secondary structure of bovine β-lactoglobulin B was determined from the circular dichroism spectra. The spectra were measured at several concentrations of surfactant ranging from 1 mg/mL to the critical micelle concentration. The surfactants studied were sodium dodecyl, decyl, and octyl sulfate, sodium dodecyl sarcosinate, dodecyltrimethylammonium bromide. The data were analyzed using the method of Chen et al. [Biochemistry (1974) 13, 3350-3359] to determine the percentage of α-helix, β-sheet, and unordered form at each surfactant concentration. In every case, an increase in structured form and a 20-25% decrease in the amount of unordered form was noted when the surfactant concentration reached the critical micelle concentration. However, the relative amounts of the two structured forms present depend on the surfactant used. The profile of the secondary structure of the protein also varied from surfactant to surfactant as the protein was titrated, probably reflecting the delicate balance between ionic and nonionic forces that governs the secondary structure of β-lactoglobulin and most other globular proteins in aqueous solution.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360210504
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