GLORIA

GEOMAR Library Ocean Research Information Access

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 21
    Publication Date: 2017-03-22
    Description: Objectives : Microscopic leukocyte differentials display many drawbacks. Several single 5 to 8-color tubes using multiparameter flow cytometry (MFC) are able to provide extended differentials with sequential gating-based analysis strategies. We investigated a new 5-color MFC method to perform an extended 8-part differential with a simplified gating strategy. Methods : Whole blood was stained with a combination of antibodies including HLA-DR-FITC/CD19-PE/CD45-ECD/CD16-PC5+CD71-PC5/CD5-PC7. Results : An original approach was developed to exclude debris and straightforwardly gate the cells to identify sixteen populations. Strong correlations were obtained with the analyzer for neutrophils, lymphocytes, monocytes, and eosinophils (R 2 〉0.9). Abnormal cells, such as immature granulocytes and blast cells were identified with a good sensitivity and excellent correlation against cytomorphologic review (R 2 =0.66 and 0.99, respectively). The choice of HLA-DR and CD5 improved specificity for the identification of activated T-lymphocytes and some lymphoid neoplastic cells, respectively. Conclusions : Here a new cytometric differential is proposed with a robust gating strategy which may be used even by unskilled cytometrists and can be easily automated. This article is protected by copyright. All rights reserved.
    Topics: Biology , Medicine
    Published by Wiley-Blackwell
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 22
    Publication Date: 2017-04-13
    Description: ABSTRACT The Cerdanya valley in northeast Spain experiences intense cold air pooling which decouples the valley atmosphere from the regional circulation, especially in winter. This makes air temperature prediction a challenge. A network of 40 temperature sensors was installed in 2012 along seven elevational transects to collect hourly temperatures throughout the cold pool, enabling measurement of the detailed cold pool structure for the first time. Sensors were also installed in the upper Conflent valley to the northeast for comparison, where previous research has shown that there is reduced cold air pooling. Sensor data were validated against automatic weather station observations at two locations. Through calculation of hourly lapse rates in various elevation bands for 2 years, frequent inversions developing up to 1450 m are shown, and sometimes extending much higher than this, concentrating in winter. Case studies of two intense episodes in December 2012 and January 2013 show that model simulations, despite being able to simulate broad mechanisms of cold air pooling formation, underestimate the amount of cooling. This is due to over-enthusiastic simulation of a low level jet in the first case and mountain waves in the second. Solving these model problems has important consequences for the future ability to predict episodes of extreme low temperatures and associated hazards (frost, fog) in Cerdanya and mountain valleys elsewhere.
    Print ISSN: 1350-4827
    Electronic ISSN: 1469-8080
    Topics: Geography , Physics
    Published by Wiley-Blackwell
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 23
    Publication Date: 2012-09-11
    Description: We present a case of multiple metastatic epithelioid sarcoma stabilized for 32 months under third line Sunitinib Malate treatment. A 34-year old male patient, native of Cameroon, presented with a 5 years history of multi-resected lesions of the right palm, which had spread on the forearm. The histopathological aspect consisted in a dermal and subcutaneous sarcomatous proliferation of atypical epithelioid and spindle cells, with foci of necrosis, and mitotic activity (9 mitoses per mm²) (Fig. 1A).
    Print ISSN: 0007-0963
    Electronic ISSN: 1365-2133
    Topics: Medicine
    Published by Wiley-Blackwell
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...