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  • 1
    Online Resource
    Online Resource
    Transfusion‐transmitted hepatitis C: A cluster of cases in transfusion‐dependent thalassaemia patients in Sri Lanka
    Wiley ; 2020
    In:  Transfusion Medicine Vol. 30, No. 5 ( 2020-10), p. 377-383
    Details
    In: Transfusion Medicine, Wiley, Vol. 30, No. 5 ( 2020-10), p. 377-383
    Abstract: To report the clinical and virologic epidemiology of a recent epidemic of hepatitis C in thalassaemia patients in Sri Lanka. Background Transfusion‐dependent thalassaemia patients remain at risk for hepatitis C virus (HCV). Here, we report a cluster of recent HCV infections in Sri Lankan thalassaemia patients and examine the phylogenetic relationship of viral sequences. Methods We conducted two prospective cross‐sectional surveys of 513 patients in four Sri Lankan thalassaemia centres in 2014/2015 and re‐surveyed one centre in 2016. We screened for anti‐HCV antibodies using the CTK Biotech enzyme‐linked immunosorbent assay (ELISA) kits and confirmed active infection by reverse transcription‐polymerase chain reaction (RT‐PCR) for HCV‐RNA. HCV genomes were sequenced by unbiased target enrichment. Results Anti‐HCV antibodies were found in 116/513 (22.6%) of patients initially tested. Active hepatitis C infection was found in 26 patients with no cases of active hepatitis B infection. Of 26 patients with HCV, two were infected with genotype 1(a), and the rest had 3(a). In a single centre (Ragama), 122 patients (120 new cases and two previously tested, but negative) were retested for anti‐HCV antibodies. 32/122 (26.2%) patients were seropositive. Twenty‐three (23/122; 18.8%) of these new cases were confirmed by HCV PCR (all genotype 3[a]). Conclusion There is a significant cluster of recent HCV cases in multiply transfused thalassaemia patients in several centres in Sri Lanka. Most of the viruses shared a close phylogenetic relationship. The results are consistent with recent continuing transfusion‐transmitted HCV infection. Routine surveillance for HCV of chronically transfused patients is required irrespective of screening of blood products.
    Type of Medium: Online Resource
    ISSN: 0958-7578 , 1365-3148
    URL: Issue
    URL: Article
    DOI: 10.1111/tme.v30.5
    DOI: 10.1111/tme.12660
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2010901-5
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  • 2
    Online Resource
    Online Resource
    Nuclear SSR markers for Miscanthus, Saccharum, and related grasses (Saccharinae, Poaceae)
    Wiley ; 2013
    In:  Applications in Plant Sciences Vol. 1, No. 11 ( 2013-11)
    Details
    In: Applications in Plant Sciences, Wiley, Vol. 1, No. 11 ( 2013-11)
    Abstract: • Premise of the study: We developed nuclear simple sequence repeat (SSR) markers for the characterization of the biomass crop Miscanthus , especially M. sacchariflorus, M. sinensis , and M. ×giganteus , and tested for cross‐species amplification. • Methods and Results: Twenty‐nine SSR markers (di‐ and tetranucleotide repeats) were developed from DNA sequences obtained from 192 clones from an enriched genomic library of M. sinensis . All markers were successfully amplified in M. sacchariflorus, M. sinensis , and M. ×giganteus , and 19 amplified across a broad range of Miscanthus species. Polymorphism information content and expected heterozygosity values (19 locus sample) were 0.88 and 0.89, respectively, for M. sinensis , 0.48 and 0.54 for M. sacchariflorus , and were the lowest in M. ×giganteus (0.33, 0.41). Thirteen out of 19 primer pairs showed cross‐species amplification in non‐ Miscanthus sensu stricto taxa. • Conclusions: The new set of 29 SSR markers will be of high value for characterizing Miscanthus germplasm collections, for prebreeding, and for assessing variation in natural populations.
    Type of Medium: Online Resource
    ISSN: 2168-0450 , 2168-0450
    URL: Article
    DOI: 10.3732/apps.1300042
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2699923-7
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