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  • Wiley  (4)
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  • Wiley  (4)
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  • 1
    Online-Ressource
    Online-Ressource
    Hyperglycolysis in endothelial cells drives endothelial injury and microvascular alterations in peritoneal dialysis
    Wiley ; 2023
    In:  Clinical and Translational Medicine Vol. 13, No. 12 ( 2023-12)
    Details
    In: Clinical and Translational Medicine, Wiley, Vol. 13, No. 12 ( 2023-12)
    Kurzfassung: Endothelial cell (EC) dysfunction leading to microvascular alterations is a hallmark of technique failure in peritoneal dialysis (PD). However, the mechanisms underlying EC dysfunction in PD are poorly defined. Methods We combined RNA sequencing with metabolite set analysis to characterize the metabolic profile of peritoneal ECs from a mouse model of PD. This was combined with EC‐selective blockade of glycolysis by genetic or pharmacological inhibition of 6‐phosphofructo‐2‐kinase/fructose‐2,6‐biphosphatase 3 (PFKFB3) in vivo and in vitro. We also investigated the association between peritoneal EC glycolysis and microvascular alterations in human peritoneal samples from patients with end‐stage kidney disease (ESKD). Results In a mouse model of PD, peritoneal ECs had a hyperglycolytic metabolism that shunts intermediates into nucleotide synthesis. Hyperglycolytic mouse peritoneal ECs displayed a unique active phenotype with increased proliferation, permeability and inflammation. The active phenotype of mouse peritoneal ECs can be recapitulated in human umbilical venous ECs and primary human peritoneal ECs by vascular endothelial growth factor that was released from high glucose‐treated mesothelial cells. Importantly, reduction of peritoneal EC glycolysis, via endothelial deficiency of the glycolytic activator PFKFB3, inhibited PD fluid‐induced increases in peritoneal capillary density, vascular permeability and monocyte extravasation, thereby protecting the peritoneum from the development of structural and functional damages. Mechanistically, endothelial PFKFB3 deficiency induced the protective effects in part by inhibiting cell proliferation, VE‐cadherin endocytosis and monocyte‐adhesion molecule expression. Pharmacological PFKFB3 blockade induced a similar therapeutic benefit in this PD model. Human peritoneal tissue from patients with ESKD also demonstrated evidence of increased EC PFKFB3 expression associated with microvascular alterations and peritoneal dysfunction. Conclusions These findings reveal a critical role of glycolysis in ECs in mediating the deterioration of peritoneal function and suggest that strategies targeting glycolysis in peritoneal ECs may be of therapeutic benefit for patients undergoing PD.
    Materialart: Online-Ressource
    ISSN: 2001-1326 , 2001-1326
    URL: Issue
    URL: Article
    DOI: 10.1002/ctm2.v13.12
    DOI: 10.1002/ctm2.1498
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2023
    ZDB Id: 2697013-2
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 2
    Online-Ressource
    Online-Ressource
    NaCl induces flocculation and lipid oxidation of soybean oil body emulsions recovered by neutral aqueous extraction
    Wiley ; 2022
    In:  Journal of the Science of Food and Agriculture Vol. 102, No. 9 ( 2022-07), p. 3752-3761
    Details
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 102, No. 9 ( 2022-07), p. 3752-3761
    Kurzfassung: Soybean oil bodies (SOB) are naturally pre‐emulsified lipid droplets recovered directly from soybean seeds. Almost all food emulsions contain salts. However, it was not clear how the incorporation of salts affected the physicochemical stability of SOB. RESULTS This study investigated the effect of NaCl (0–1.2%) on the physical and oxidative stability of SOB emulsions under neutral (pH 7) and acidic (pH 3) conditions. In the presence of NaCl, the SOB emulsion (pH 7) showed strong flocculation during storage due to electrostatic screening. The NaCl‐induced flocculation of SOB was attenuated at pH 3, which may be due to the difference in conformation or interaction of the protein interfaces covering SOB at different pH values. The increase in ionic strength or acid conditioning treatment resulted in a remarkable increase in the stability of SOB emulsions against coalescence. The confocal laser scanning microscopy images also confirmed the NaCl‐induced changes in the flocculation/coalescence properties of SOB. The oxidative behavior tests indicated that SOB emulsions containing NaCl were more susceptible to lipid oxidation but protein oxidation was inhibited due to electrostatic screening, which reduced pro‐oxidant accessibility of unadsorbed proteins in the emulsion. This oxidative behavior was attenuated at pH 3. CONCLUSION The incorporation of NaCl significantly reduced the physical and oxidative stability of the SOB emulsion, and acidic pH mitigated NaCl‐induced flocculation and lipid oxidation of SOB. © 2021 Society of Chemical Industry.
    Materialart: Online-Ressource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    URL: Article
    DOI: 10.1002/jsfa.v102.9
    DOI: 10.1002/jsfa.11723
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2022
    ZDB Id: 2001807-1
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 3
    Online-Ressource
    Online-Ressource
    Ziziphi s pinosae Semen Oil Enhance the Oxidative Stability of Soybean Oil under Frying Conditions
    Wiley ; 2021
    In:  European Journal of Lipid Science and Technology Vol. 123, No. 11 ( 2021-11)
    Details
    In: European Journal of Lipid Science and Technology, Wiley, Vol. 123, No. 11 ( 2021-11)
    Kurzfassung: The objective of this study is to improve the oxidative stability of soybean oil by using Ziziphi spinosae semen oil (ZSSO). In the present study, the oxidative stability, fatty acid composition, tocopherol, and phenolic changes of soybean oil without additives and soybean oil mixed with 5% ZSSO are evaluated during frying at 180 ℃ for 18 h. Tert‐butyl hydroquinone (TBHQ) and vitamin E (VE) as common antioxidants are incorporated into soybean oil for comparison. According to the results of oxidative stability assays of conjugated diene value, thiobarbituric acid value, acid values, and total polar compounds, the incorporation of ZSSO significantly restrain the lipid oxidation of soybean oil. After 18 h of frying, the soybean oil samples with ZSSO has more polyunsaturated fatty acids, tocopherols, and DPPH radical scavenging capacity, and less trans fatty acids, compared with TBHQ and VE. In addition, ZSSO‐containing soybean oil maintains a high content of phenols during the frying period, which is correlated with the increase in oxidative stability. Therefore, replacing part of soybean oil with ZSSO can effectively reinforce the performance of soybean oil under frying conditions.
    Materialart: Online-Ressource
    ISSN: 1438-7697 , 1438-9312
    URL: Issue
    URL: Article
    DOI: 10.1002/ejlt.v123.11
    DOI: 10.1002/ejlt.202100060
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2021
    ZDB Id: 2012720-0
    SSG: 21
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 4
    Online-Ressource
    Online-Ressource
    Association of Urinary Matrix Metalloproteinase 7 Levels With Incident Renal Flare in Lupus Nephritis
    Wiley ; 2021
    In:  Arthritis & Rheumatology Vol. 73, No. 2 ( 2021-02), p. 265-275
    Details
    In: Arthritis & Rheumatology, Wiley, Vol. 73, No. 2 ( 2021-02), p. 265-275
    Kurzfassung: Flares of lupus nephritis (LN) are frequent and associated with impaired renal prognosis. One major management obstacle in LN flare is the lack of effective methods to identify at‐risk patients earlier in their disease course. This study was undertaken to test the utility of measurement of urinary matrix metalloproteinase 7 (MMP‐7) for the dynamic surveillance of renal disease activity and prediction of renal flares in LN. Methods A prospective, 2‐stage cohort study was performed in patients with LN. Urinary MMP‐7 levels at the time of biopsy were evaluated in 154 patients with newly diagnosed LN in 2 independent cohorts. Urinary MMP‐7 levels were assessed for correlation with renal histologic activity. Furthermore, after a minimum period of 12 months of renal disease remission, urinary MMP‐7 levels were monitored bimonthly for 2 years in 65 patients with LN. The association between urinary MMP‐7 levels and development of LN flare was analyzed. Results Urinary MMP‐7 levels were elevated in patients with LN. A higher urinary MMP‐7 level in LN was associated with greater renal histologic activity. As a marker for identifying LN patients with more severe renal histologic activity (i.e., a histologic activity index of ≥7), the level of urinary MMP‐7 outperformed other clinical markers and improved their predictive performance, thus linking urinary MMP‐7 levels to renal disease activity. Furthermore, among patients who had follow‐up measurements of urinary MMP‐7 after achievement of long‐term remission of renal disease activity, an elevated urinary MMP‐7 level during follow‐up was independently associated with an increased risk of LN flare. This elevation in the urinary MMP‐7 level hinted at the risk of an LN flare at an earlier time point prior to indications using conventional laboratory measures. Thus, use of the urinary MMP‐7 level in conjunction with other clinical measures improved the prognostic value for prediction of an LN flare. Conclusion Urinary MMP‐7 levels in LN are correlated with renal histologic activity. An elevated urinary MMP‐7 level detected after achievement of long‐term renal disease remission is associated with a higher risk of incident renal flare in patients with LN.
    Materialart: Online-Ressource
    ISSN: 2326-5191 , 2326-5205
    URL: Issue
    URL: Article
    DOI: 10.1002/art.v73.2
    DOI: 10.1002/art.41506
    RVK:
    XA 10000
    RVK:
    XA 30325
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2021
    ZDB Id: 2754614-7
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
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