In:
Clinical and Translational Science, Wiley, Vol. 13, No. 2 ( 2020-03), p. 265-274
Abstract:
Nasopharyngeal carcinoma ( NPC ) is a common cancer found in the nasopharynx with high metastatic and invasive nature. Increasing evidences have identified the critical role of gene therapy in NPC treatment. Hence, this study was designed to identify specific gene markers that affected NPC progression through gene expression profile analysis. NPC ‐related gene expression data set gene set enrichment ( GSE )53819 were retrieved and analyzed to screen out differentially expressed genes ( DEG s), followed by determination of their expression in noncancerous tissues and NPC specimens. Next, weighted gene co‐expression network analysis ( WGCNA ) was conducted on DEG s to obtain tumor‐associated gene modules. Genes in those modules were intersected with DEG s for gene ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analysis. Then protein‐protein interaction network of tumor‐associated genes was constructed to select genes most closely linked to NPC . Afterward, expression of chromosome 9 open reading frame 24 (c9orf24), primary ciliary dyskinesia protein 1 ( PCDP 1), and leucine‐rich repeat‐containing protein 46 ( LRRC 46) was detected in GSE 53819 and further verified in GSE 12452 and GSE 64634. Differential analysis on GSE 53819 found that 2,173 genes were aberrantly expressed in NPC , among which 917 genes are upregulated and 1,256 genes are downregulated. WGCNA showed that genes were enriched in 17 modules and 727 genes exhibited ectopic expression in NPC and enriched in cytokine‐cytokine receptor interaction, cytochrome P450, and chemical carcinogenesis signaling pathways, among which c9orf24, PCDP 1, and LRRC 46 were poorly expressed in NPC . Therefore, c9orf24, PCDP 1, and LRRC 46 might serve as prominent diagnostic markers for NPC , which presents new insights for NPC therapy.
Type of Medium:
Online Resource
ISSN:
1752-8054
,
1752-8062
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2433157-0
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