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  • 1
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    Impact of LPL gene rs283 polymorphism on exercise‐induced changes in metabolism of obese adolescents and the regulatory mechanisms behind it
    Wiley ; 2015
    In:  Experimental Physiology Vol. 100, No. 6 ( 2015-06), p. 698-707
    Details
    In: Experimental Physiology, Wiley, Vol. 100, No. 6 ( 2015-06), p. 698-707
    Abstract: What is the central question of this study? We investigated whether the LPL gene rs283 polymorphism affects exercise‐induced changes in body composition and lipid and glucose metabolism in obese adolescents and whether it is functional. What is the main finding and its importance? Chinese obese adolescents of Han nationality with the GG genotype of the rs283 polymorphism were more sensitive to exercise‐induced reduction of the body fat percentage, insulin resistance and plasma triglyceride levels. The G allele can significantly increase reporter gene expression level, which may be the molecular reason for the difference in exercise‐induced parameter changes among obese adolescents. The aim of this investigation was to explore the association between the rs283 polymorphism located in the lipoprotein lipase ( LPL ) gene and exercise‐induced changes in body composition and lipid and glucose metabolism in obese adolescents and to probe into the molecular regulatory mechanisms. Fifty‐five obese adolescents of Han nationality underwent aerobic training for 4 weeks. Body composition and lipid and glucose metabolic parameters were tested before and after the training. The rs283 polymorphism was genotyped by PCR–restriction fragment length polymorphism, and association analysis with the weight‐reducing effect was performed. The regulatory mechanisms of the rs283 polymorphism were explored through the dual‐luciferase reporter assay. Exercise‐induced change rates were as follows: the change in body fat percentage of GG genotype groups was 3.37 ± 1.60, significantly higher than that of GA genotype groups (2.09 ± 1.53, P   〈  0.01); the change in the homeostasis model assessment of insulin resistance was 0.52 ± 0.13, obviously higher than that of GA genotype groups (0.44 ± 0.10, P   〈  0.05); and the change in triglyceride was 51.91 ± 6.56, much higher than that of GA genotype groups (47.06 ± 5.36, P   〈  0.01). The relative luciferase activity of the reporter gene in recombinant vector carrying the G allele was 2.67 ± 0.22, markedly higher than that in recombinant vector carrying the A allele (1.63 ± 0.03, P   〈  0.01). Chinese obese adolescents of Han nationality with GG genotype of the rs283 polymorphism were more sensitive to exercise‐induced parameter changes. The G allele can improve reporter gene expression level, indicating the effects of rs283 on gene expression.
    Type of Medium: Online Resource
    ISSN: 0958-0670 , 1469-445X
    URL: Issue
    URL: Article
    DOI: 10.1113/eph.2015.100.issue-6
    DOI: 10.1113/EP085127
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1493802-9
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  • 2
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    Development and Validation of Noninvasive MRI ‐Based Signature for Preoperative Prediction of Early Recurrence in Perihilar Cholangiocarcinoma
    Wiley ; 2022
    In:  Journal of Magnetic Resonance Imaging Vol. 55, No. 3 ( 2022-03), p. 787-802
    Details
    In: Journal of Magnetic Resonance Imaging, Wiley, Vol. 55, No. 3 ( 2022-03), p. 787-802
    Abstract: Cholangiocarcinoma is a type of hepatobiliary tumor. For perihilar cholangiocarcinoma (pCCA), patients who experience early recurrence (ER) have a poor prognosis. Preoperative accurate prediction of postoperative ER can avoid unnecessary operation; however, prediction is challenging. Purpose To develop a novel signature based on clinical and/or MRI radiomics features of pCCA to preoperatively predict ER. Study Type Retrospective. Population One hundred eighty‐four patients (median age, 61.0 years; interquartile range: 53.0–66.8 years) including 115 men and 69 women. Field Strength/Sequence A 1.5 T; volumetric interpolated breath‐hold examination ( VIBE ) sequence. Assessment The models were developed from the training set (128 patients) and validated in a separate testing set (56 patients). The contrast‐enhanced arterial and portal vein phase MR images of hepatobiliary system were used for extracting radiomics features. The correlation analysis, least absolute shrinkage and selection operator (LASSO) logistic regression (LR), backward stepwise LR were mainly used for radiomics feature selection and modeling (Model radiomic ). The univariate and multivariate backward stepwise LR were used for preoperative clinical predictors selection and modeling (Model clinic ). The radiomics and preoperative clinical predictors were combined by multivariate LR method to construct clinic‐radiomics nomogram (Model combine ). Statistical Tests Chi‐squared ( χ 2 ) test or Fisher's exact test, Mann–Whitney U ‐test or t ‐test, Delong test. Two tailed P   〈  0.05 was considered statistically significant. Results Based on the comparison of area under the curves (AUC) using Delong test, Model clinic and Model combine had significantly better performance than Model radiomic and tumor‐node‐metastasis (TNM) system in training set. In the testing set, both Model clinic and Model combine had significantly better performance than TNM system, whereas only Model combine was significantly superior to Model radiomic . However, the AUC values were not significantly different between Model clinic and Model combine ( P  = 0.156 for training set and P  = 0.439 for testing set). Data Conclusion A noninvasive model combining the MRI‐based radiomics signature and clinical variables is potential to preoperatively predict ER for pCCA. Level of Evidence 3 Technical Efficacy Stage 4
    Type of Medium: Online Resource
    ISSN: 1053-1807 , 1522-2586
    URL: Issue
    URL: Article
    DOI: 10.1002/jmri.v55.3
    DOI: 10.1002/jmri.27846
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 1497154-9
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  • 3
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    T2* Mapping to characterize intestinal fibrosis in crohn's disease
    Wiley ; 2018
    In:  Journal of Magnetic Resonance Imaging Vol. 48, No. 3 ( 2018-09), p. 829-836
    Details
    In: Journal of Magnetic Resonance Imaging, Wiley, Vol. 48, No. 3 ( 2018-09), p. 829-836
    Abstract: Assessing bowel fibrosis in patients with Crohn's disease (CD) has important therapeutic implications. PURPOSE To determine the utility of T2* mapping versus that of contrast enhanced (CE) imaging in grading intestinal fibrosis in patients with CD using surgical pathology as the reference standard. STUDY TYPE Prospective. SPECIMENS 102 specimens from 27 patients with CD. FIELD STRENGTH/SEQUENCE 3.0T; T2WI; T1WI; T2*WI. ASSESSMENT The T2*WI values of the bowel wall targeted for resection were measured by two radiologists by drawing regions of interest on the thickened bowel wall. The resected bowel specimens with pathological fibrosis and type I collagen were classified into four severity grades (0–3) by a pathologist using a semi‐quantitative scoring system. STATISTICAL TESTS The differences in the T2*WI values among the different histological grades were analyzed using one‐way analysis of variance or the Kruskal‐Wallis test, and their correlations were analyzed. The ability of the T2*WI values to discriminate between various degrees of fibrosis was assessed using a receiver operating characteristic (ROC) curve. RESULTS Significant differences were observed in the T2* values of mild (23.56 ± 1.60 ms), moderate (16.19 ± 0.55 ms), and severe (13.59 ± 0.53 ms) fibrosis types ( F  = 35.84; P   〈  0.001). T2* values were moderately associated with histological fibrosis ( r  = ‐0.627; P   〈  0.001) and type I collagen scores ( r  = ‐0.588; P   〈  0.001). T2* values were highly accurate, with an area under the ROC curve (AUC) of 0.951 ( P   〈  0.001) for differentiating moderate‐to‐severe fibrosis from nonfibrosis and mild fibrosis, followed by an AUC of 0.508 for the percentage of enhancement gain ( P  = 0.908). A threshold T2* value of 18.06 ms was recommended for diagnosing moderate‐to‐severe fibrosis with 94.7% sensitivity and 78.3% specificity. DATA CONCLUSION MRI T2* mapping outperforms CE parameters in distinction of various degrees of bowel fibrosis in CD. Level of Evidence: 1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2018;48:829–836.
    Type of Medium: Online Resource
    ISSN: 1053-1807 , 1522-2586
    URL: Issue
    URL: Article
    DOI: 10.1002/jmri.v48.3
    DOI: 10.1002/jmri.26022
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1497154-9
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  • 4
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    Diversification of Rosaceae since the Late Cretaceous based on plastid phylogenomics
    Wiley ; 2017
    In:  New Phytologist Vol. 214, No. 3 ( 2017-05), p. 1355-1367
    Details
    In: New Phytologist, Wiley, Vol. 214, No. 3 ( 2017-05), p. 1355-1367
    Abstract: Phylogenetic relationships in Rosaceae have long been problematic because of frequent hybridisation, apomixis and presumed rapid radiation, and their historical diversification has not been clarified. With 87 genera representing all subfamilies and tribes of Rosaceae and six of the other eight families of Rosales (outgroups), we analysed 130 newly sequenced plastomes together with 12 from GenBank in an attempt to reconstruct deep relationships and reveal temporal diversification of this family. Our results highlight the importance of improving sequence alignment and the use of appropriate substitution models in plastid phylogenomics. Three subfamilies and 16 tribes (as previously delimited) were strongly supported as monophyletic, and their relationships were fully resolved and strongly supported at most nodes. Rosaceae were estimated to have originated during the Late Cretaceous with evidence for rapid diversification events during several geological periods. The major lineages rapidly diversified in warm and wet habits during the Late Cretaceous, and the rapid diversification of genera from the early Oligocene onwards occurred in colder and drier environments. Plastid phylogenomics offers new and important insights into deep phylogenetic relationships and the diversification history of Rosaceae. The robust phylogenetic backbone and time estimates we provide establish a framework for future comparative studies on rosaceous evolution.
    Type of Medium: Online Resource
    ISSN: 0028-646X , 1469-8137
    URL: Issue
    URL: Article
    DOI: 10.1111/nph.2017.214.issue-3
    DOI: 10.1111/nph.14461
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 208885-X
    detail.hit.zdb_id: 1472194-6
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  • 5
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    Photoresponsive Solid Nanochannels Membranes: Design and Applications
    Wiley ; 2022
    In:  Small Vol. 18, No. 12 ( 2022-03)
    Details
    In: Small, Wiley, Vol. 18, No. 12 ( 2022-03)
    Abstract: Light stimuli have notable advantages over other environmental stimuli, such as more precise spatial and temporal regulation, and the ability to serve as an energy source to power the system. In nature, photoresponsive nanochannels are important components of organisms, with examples including the rhodopsin channels in optic nerve cells and photoresponsive protein channels in the photosynthesis system of plants. Inspired by biological channels, scientists have constructed various photoresponsive, smart solid‐state nanochannels membranes for a range of applications. In this review, the methods and applications of photosensitive nanochannels membranes are summarized. The authors believe that this review will inspire researchers to further develop multifunctional artificial nanochannels for applications in the fields of biosensors, stimuli‐responsive smart devices, and nanofluidic devices, among others.
    Type of Medium: Online Resource
    ISSN: 1613-6810 , 1613-6829
    URL: Issue
    URL: Article
    DOI: 10.1002/smll.v18.12
    DOI: 10.1002/smll.202105019
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2168935-0
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  • 6
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    Diffusion kurtosis MRI versus conventional diffusion‐weighted imaging for evaluating inflammatory activity in Crohn's disease
    Wiley ; 2018
    In:  Journal of Magnetic Resonance Imaging Vol. 47, No. 3 ( 2018-03), p. 702-709
    Details
    In: Journal of Magnetic Resonance Imaging, Wiley, Vol. 47, No. 3 ( 2018-03), p. 702-709
    Abstract: To assess the efficacy of diffusion kurtosis imaging (DKI) and to compare DKI‐derived parameters with that of conventional diffusion‐weighted imaging (DWI) for grading the inflammatory activity of Crohn's disease (CD). Materials and Methods In all, 38 patients with CD underwent 3T magnetic resonance enterography (MRE) with DKI (b values of 0–2000 s/mm 2 ). The inflammatory activity of the bowel segments was graded by magnetic resonance index of activity (MaRIA) as inactive ( 〈 7), mild (≥7 and 〈 11), or moderate‐severe (≥11). Apparent diffusion for non‐Gaussian distribution (D app ) and apparent kurtosis coefficient (K app ) on DKI as well as apparent diffusion coefficient (ADC) on DWI were compared. Results In all, 86 bowel segments including inactive (20), mild (19), and moderate‐severe (47) CD were analyzed. The differences in K app , D app , and ADC among inactive, mild, and moderate‐severe CD were significant (all P 〈 0.05). K app ( r = 0.862), D app ( r = −0.755), and ADC ( r = −0.713) correlated well with MaRIA in all segments. Stronger correlation with MaRIA in moderate‐severe CD was found for K app ( r = 0.647) than that of D app ( r = −0.414) and ADC ( r = −0.580). Receiver operating characteristic (ROC) curve analysis showed high accuracy of K app , D app , and ADC for differentiating active from inactive CD (AUC: 0.953 for K app , 0.944 for D app , 0.907 for ADC) as well as differentiating inactive‐mild from moderate‐severe CD (AUC: 0.946 for K app , 0.887 for D app , 0.846 for ADC). The threshold K app of 0.731 allowed differentiation of active from inactive CD with 89.4% sensitivity and 95% specificity. Conclusion DKI of CD is clinically feasible and might be superior to conventional DWI for grading the inflammatory activity of CD. Level of Evidence : 2 Technical Efficacy : Stage 2 J. Magn. Reson. Imaging 2018;47:702–709.
    Type of Medium: Online Resource
    ISSN: 1053-1807 , 1522-2586
    URL: Issue
    URL: Article
    DOI: 10.1002/jmri.v47.3
    DOI: 10.1002/jmri.25768
    Language: English
    Publisher: Wiley
    Publication Date: 2018
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  • 7
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    Single‐Cell Transcriptome Analysis Uncovers Intratumoral Heterogeneity and Underlying Mechanisms for Drug Resistance in Hepatobiliary Tumor Organoids
    Wiley ; 2021
    In:  Advanced Science Vol. 8, No. 11 ( 2021-06)
    Details
    In: Advanced Science, Wiley, Vol. 8, No. 11 ( 2021-06)
    Abstract: Molecular heterogeneity of hepatobiliary tumor including intertumoral and intratumoral disparity always leads to drug resistance. Here, seven hepatobiliary tumor organoids are generated to explore heterogeneity and evolution via single‐cell RNA sequencing. HCC272 with high status of epithelia‐mesenchymal transition proves broad‐spectrum drug resistance. By examining the expression pattern of cancer stem cells markers (e.g., PROM1, CD44, and EPCAM), it is found that CD44 positive population may render drug resistance in HCC272. UMAP and pseudo‐time analysis identify the intratumoral heterogeneity and distinct evolutionary trajectories, of which catenin beta‐1 (CTNNB1), glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), and nuclear paraspeckle assembly transcript 1 (NEAT1) advantage expression clusters are commonly shared across hepatobiliary organoids. CellphoneDB analysis further implies that metabolism advantage organoids with enrichment of hypoxia signal upregulate NEAT1 expression in CD44 subgroup and mediate drug resistance that relies on Jak‐STAT pathway. Moreover, metabolism advantage clusters shared in several organoids have similar characteristic genes (GAPDH, NDRG1 (N‐Myc downstream regulated 1), ALDOA, and CA9). The combination of GAPDH and NDRG1 is an independent risk factor and predictor for patient survival. This study delineates heterogeneity of hepatobiliary tumor organoids and proposes that the collaboration of intratumoral heterogenic subpopulations renders malignant phenotypes and drug resistance.
    Type of Medium: Online Resource
    ISSN: 2198-3844 , 2198-3844
    URL: Issue
    URL: Article
    DOI: 10.1002/advs.v8.11
    DOI: 10.1002/advs.202003897
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2808093-2
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  • 8
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    Antennal and behavioral responses of female M aruca vitrata to the floral volatiles of V igna unguiculata and L ablab purpureus
    Wiley ; 2014
    In:  Entomologia Experimentalis et Applicata Vol. 152, No. 3 ( 2014-09), p. 248-257
    Details
    In: Entomologia Experimentalis et Applicata, Wiley, Vol. 152, No. 3 ( 2014-09), p. 248-257
    Abstract: In many herbivorous insects, the attraction to suitable host plants is in part mediated by similar olfactory cues from different host plants. This study investigated the electrophysiological and behavioral responses of female M aruca vitrata F abricius ( L epidoptera: C rambidae) to floral volatiles from two host plants, V igna unguiculata ( L .) W alpers and L ablab purpureus ( L .) S weet (both F abaceae). Qualitative and quantitative differences were found between the odor profiles of two host plants by gas chromatography‐mass spectrometry ( GC ‐ MS ). Nine electroantennography‐active compounds were detected in the headspace collections of the two host plants by means of coupled GC ‐electroantennographic detection. In a wind tunnel, female behaviors (upwind flight and source approach) were not affected by stimuli or their interaction with host plants, but the behaviors were influenced by host plants. A mixture of the nine bioactive compounds, including limonene, 1,3‐diethylbenzene, benzaldehyde, acetophenone, 4‐ethylbenzaldehyde, 1‐(4‐ethylphenyl)‐ethanone, 2‐methyl‐3‐phenylpropanal, 1H‐indol‐4‐ol, and 1,1′‐(1,4‐phenylene)bis‐ethanone mimicking L . purpureus (in a ratio of 0.4:9.7:2.4:5.7:78.1:4.8:100:0.6:30.3) attracted significantly more females approaching the sources compared with the mixture of compounds mimicking V . unguiculata (in a ratio of 0.3:1.4:1.6:3.7:50.8:3.2:100:4.2:16.7). Further bioassays revealed that a subtractive 4‐component blend [limonene, 1,3‐diethylbenzene, 4‐ethylbenzaldehyde, and 1‐(4‐ethylphenyl)‐ethanone in a ratio of 0.4:9.7:78.1:4.8] was essential for maximal attraction. Our study suggested that female M . vitrata might use similar odors from V . unguiculata and L . purpureus to locate suitable hosts and that an artificial lure, containing the major floral volatiles released by the two host plants, might be useful in exploring efficiency monitoring and/or control strategies of this moth in the field.
    Type of Medium: Online Resource
    ISSN: 0013-8703 , 1570-7458
    URL: Issue
    URL: Article
    DOI: 10.1111/eea.2014.152.issue-3
    DOI: 10.1111/eea.12216
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 2015286-3
    SSG: 12
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  • 9
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    Synthesis of Ag Nanorods with Highly Tunable Plasmonics toward Optimal Surface‐Enhanced Raman Scattering Substrates Self‐Assembled at Interfaces
    Wiley ; 2017
    In:  Advanced Optical Materials Vol. 5, No. 21 ( 2017-11)
    Details
    In: Advanced Optical Materials, Wiley, Vol. 5, No. 21 ( 2017-11)
    Abstract: Ag nanorods receive intensive attention due to the excellent plasmonic properties. However, the difficulty in synthesis of monodisperse Ag nanorods with broad aspect ratios has limited their in‐depth applications. Here, a seed‐mediated method is reported for the synthesis of Ag nanorods with lengths from 65 to 5000 nm, corresponding to aspect ratios from 2 to 156. The plasmonic resonance is tuned from visible to mid‐infrared wavelength. The synthesis protocol relies on robust Au seeds synthesized in N , N ‐dimethylformamide (DMF), which induces the one‐dimensional (1D) growth of Ag atoms. To maintain symmetry breaking initiated by the Au seeds, the reduction rate of Ag + is decreased by adding hexadecyltrimethylammonium chloride (CTAC) to form AgCl particles. The optimized conditions to prevent the homogeneous nucleation of Ag nanoparticles and residue of AgCl particles in products are identified, under which the conversion efficiency of Ag ions to Ag nanorods is evaluated about 48%. More importantly, the anisotropic Ag nanorods are self‐assembled into monolayers at interfaces with the long axis of Ag nanorods perpendicular or parallel to the interfaces, respectively. The as‐fabricated monolayers exhibit uniform and reproducible surface‐enhanced Raman scattering (SERS) activities. The optimal SERS performance is achieved from Ag nanorod monolayer with vertical orientation and the longest rod length.
    Type of Medium: Online Resource
    ISSN: 2195-1071 , 2195-1071
    URL: Issue
    URL: Article
    DOI: 10.1002/adom.v5.21
    DOI: 10.1002/adom.201700581
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2708158-8
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  • 10
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    Electrochemical Exfoliation of Pillared‐Layer Metal–Organic Framework to Boost the Oxygen Evolution Reaction
    Wiley ; 2018
    In:  Angewandte Chemie International Edition Vol. 57, No. 17 ( 2018-04-16), p. 4632-4636
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 57, No. 17 ( 2018-04-16), p. 4632-4636
    Abstract: Two‐dimensional (2D) materials and ultrathin nanosheets are advantageous for elevating the catalysis performance and elucidating the catalysis mechanism of heterogeneous catalysts, but they are mostly restricted to inorganic or organic materials based on covalent bonds. We report an electrochemical/chemical exfoliation strategy for synthesizing metal–organic 2D materials based on coordination bonds. A catechol functionalized ligand is used as the redox active pillar to construct a pillared‐layer framework. When the 3D pillared‐layer MOF serves as an electrocatalyst for water oxidation (pH 13), the pillar ligands can be oxidized in situ and removed. The remaining ultrathin (2 nm) nanosheets of the metal–organic layers are an efficient catalyst with overpotentials as low as 211 mV at 10 mA cm −2 and a turnover frequency as high as 30 s −1 at an overpotential of 300 mV.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v57.17
    DOI: 10.1002/anie.201801029
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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