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  • 1
    Online Resource
    Online Resource
    Cross‐talk between EGF and BMP 9 signalling pathways regulates the osteogenic differentiation of mesenchymal stem cells
    Wiley ; 2013
    In:  Journal of Cellular and Molecular Medicine Vol. 17, No. 9 ( 2013-09), p. 1160-1172
    Details
    In: Journal of Cellular and Molecular Medicine, Wiley, Vol. 17, No. 9 ( 2013-09), p. 1160-1172
    Abstract: Mesenchymal stem cells ( MSC s) are multipotent progenitors, which give rise to several lineages, including bone, cartilage and fat. Epidermal growth factor ( EGF ) stimulates cell growth, proliferation and differentiation. EGF acts by binding with high affinity to epidermal growth factor receptor ( EGFR ) on the cell surface and stimulating the intrinsic protein tyrosine kinase activity of its receptor, which initiates a signal transduction cascade causing a variety of biochemical changes within the cell and regulating cell proliferation and differentiation. We have identified BMP 9 as one of the most osteogenic BMP s in MSC s. In this study, we investigate if EGF signalling cross‐talks with BMP 9 and regulates BMP 9‐induced osteogenic differentiation. We find that EGF potentiates BMP 9‐induced early and late osteogenic markers of MSC s in vitro , which can be effectively blunted by EGFR inhibitors Gefitinib and Erlotinib or receptor tyrosine kinase inhibitors AG ‐1478 and AG ‐494 in a dose‐ and time‐dependent manner. Furthermore, EGF significantly augments BMP 9‐induced bone formation in the cultured mouse foetal limb explants. In vivo stem cell implantation experiment reveals that exogenous expression of EGF in MSC s can effectively potentiate BMP 9‐induced ectopic bone formation, yielding larger and more mature bone masses. Interestingly, we find that, while EGF can induce BMP 9 expression in MSC s, EGFR expression is directly up‐regulated by BMP 9 through Smad1/5/8 signalling pathway. Thus, the cross‐talk between EGF and BMP 9 signalling pathways in MSC s may underline their important roles in regulating osteogenic differentiation. Harnessing the synergy between BMP 9 and EGF should be beneficial for enhancing osteogenesis in regenerative medicine.
    Type of Medium: Online Resource
    ISSN: 1582-1838 , 1582-4934
    URL: Issue
    URL: Article
    DOI: 10.1111/jcmm.2013.17.issue-9
    DOI: 10.1111/jcmm.12097
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2076114-4
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  • 2
    Online Resource
    Online Resource
    IDH Mutation Subgroup Status Associates with Intratumor Heterogeneity and the Tumor Microenvironment in Intrahepatic Cholangiocarcinoma
    Wiley ; 2021
    In:  Advanced Science Vol. 8, No. 17 ( 2021-09)
    Details
    In: Advanced Science, Wiley, Vol. 8, No. 17 ( 2021-09)
    Abstract: Intrahepatic cholangiocarcinoma (ICC) is highly heterogeneous. Here, the authors perform exome sequencing and bulk RNA sequencing on 73 tumor regions from 14 ICC patients to portray the multi‐faceted intratumor heterogeneity (ITH) landscape of ICC. The authors show that ITH is highly concordant across genomic, transcriptomic, and immune levels. Comparison of these data to 8 published datasets reveals significantly higher degrees of ITH in ICC than hepatocellular carcinoma. Remarkably, the authors find that high‐ITH tumors highly overlap with the IDH (isocitrate dehydrogenase)‐mutant subgroup ( IDH ‐SG), comprising of IDH ‐mutated tumors and IDH ‐like tumors, that is, those IDH ‐wildtype tumors that exhibit similar molecular profiles to the IDH ‐mutated ones. Furthermore, IDH ‐SG exhibits less T cell infiltration and lower T cell cytotoxicity, indicating a colder tumor microenvironment (TME). The higher ITH and colder TME of IDH ‐SG are successfully validated by single‐cell RNA sequencing on 17 503 cells from 4 patients. Collectively, the study shows that IDH mutant subgroup status, rather than IDH mutation alone, is associated with ITH and the TME of ICC tumors. The results highlight that IDH ‐like patients may also benefit from IDH targeted therapies and provide important implications for the diagnosis and treatment of ICC.
    Type of Medium: Online Resource
    ISSN: 2198-3844 , 2198-3844
    URL: Issue
    URL: Article
    DOI: 10.1002/advs.v8.17
    DOI: 10.1002/advs.202101230
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2808093-2
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  • 3
    Online Resource
    Online Resource
    Noggin resistance contributes to the potent osteogenic capability of BMP9 in mesenchymal stem cells
    Wiley ; 2013
    In:  Journal of Orthopaedic Research Vol. 31, No. 11 ( 2013-11), p. 1796-1803
    Details
    In: Journal of Orthopaedic Research, Wiley, Vol. 31, No. 11 ( 2013-11), p. 1796-1803
    Abstract: Mesenchymal stem cells (MSCs) are multipotent progenitors and can differentiate into osteogenic, chondrogenic, and adipogenic lineages. Bone morphogenetic proteins (BMPs) play important roles in stem cell proliferation and differentiation. We recently demonstrated that BMP9 is a potent but less understood osteogenic factor. We previously found that BMP9‐induced ectopic bone formation is not inhibited by BMP3. Here, we investigate the effect of BMP antagonist noggin on BMP9‐induced osteogenic differentiation. BMP antagonists noggin, chording, gremlin, follistatin, and BMP3 are highly expressed in MSCs, while noggin and follistatin are lowly expressed in more differentiated pre‐osteoblast C2C12 cells. BMP9‐induced osteogenic markers and matrix mineralization are not inhibited by noggin, while noggin blunts BMP2, BMP4, BMP6, and BMP7‐induced osteogenic markers and mineralization. Likewise, ectopic bone formation by MSCs transduced with BMP9, but not the other four BMPs, is resistant to noggin inhibition. BMP9‐induced nuclear translocation of Smad1/5/8 is not affected by noggin, while noggin blocks BMP2‐induced activation of Smad1/5/8 in MSCs. Noggin fails to inhibit BMP9‐induced expression of downstream targets in MSCs. Thus, our results strongly suggest that BMP9 may effectively overcome noggin inhibition, which should at least in part contribute to BMP9's potent osteogenic capability in MSCs. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31:1796–1803, 2013
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    URL: Article
    DOI: 10.1002/jor.v31.11
    DOI: 10.1002/jor.22427
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2050452-4
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  • 4
    Online Resource
    Online Resource
    Gain Properties and Distributed Feedback Laser Performance of 7F6/Poly(Styrene) Blend Films: Potential Core Material for Plastic Optical Fiber Expanding the Bandwidth to Visible Region
    Wiley ; 2018
    In:  Macromolecular Chemistry and Physics Vol. 219, No. 7 ( 2018-04)
    Details
    In: Macromolecular Chemistry and Physics, Wiley, Vol. 219, No. 7 ( 2018-04)
    Abstract: Optical gain properties of blue emission oligomer 7‐unit 9,9‐dihexylfluorene (7F6) and its blends with polystyrene (PS) are reported. 7F6 demonstrates high photoluminescence quantum yield (65%), low amplified spontaneous emission threshold ( E th ASE = 0.6 kW cm −2 ), high gain coefficient ( g = 90.9 cm −1 ), and extremely low distributed feedback laser threshold ( E th laser = 86 W cm −2 ). Unlike polymer gain materials, the phase separation between 7F6 and PS is small even in a high blending ratio. The 70 and 50 wt% 7F6/PS blends display excellent gain properties with g = 70.7 and 64.3 cm −1 , E th ASE = 1.1 and 2.1 kW cm −2 , and E th laser = 0.3 and 0.41 kW cm −2 , respectively. The photostability and thermal stability are improved significantly by blending 7F6 into PS. These results promise 7F6/PS blends as a potential core material for expanding the bandwidth of plastic optical fiber.
    Type of Medium: Online Resource
    ISSN: 1022-1352 , 1521-3935
    URL: Issue
    URL: Article
    DOI: 10.1002/macp.v219.7
    DOI: 10.1002/macp.201700527
    RVK:
    VA 5810
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1475026-0
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  • 5
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    Online Resource
    Facile and Controllable Fabrication of High‐Performance Methylammonium Lead Triiodide Films Using Lead Acetate Precursor for Low‐Threshold Amplified Spontaneous Emission and Distributed‐Feedback Lasers
    Wiley ; 2019
    In:  physica status solidi (RRL) – Rapid Research Letters Vol. 13, No. 10 ( 2019-10)
    Details
    In: physica status solidi (RRL) – Rapid Research Letters, Wiley, Vol. 13, No. 10 ( 2019-10)
    Abstract: Organic–inorganic lead halide perovskites have emerged rapidly as the most attractive materials for photovoltaics in the last 10 years. Intense research has been done on crystal growth and morphology control to improve their power conversion efficiencies. Furthermore, perovskites also show great potential for optical amplification and lasing. Despite the numerous reports on how processing conditions affect the perovskite light‐harvesting properties, effects on amplified spontaneous emission (ASE) or lasing performance have attracted considerably less attention. Herein, a detailed study on the ASE performance of methylammonium lead triiodide (MAPbI 3 ) films, processed with lead acetate (Pb(Ac) 2 ) as lead source following a one‐step spin‐coating method and exposed to different post‐deposition conditions, is presented. It is found that the use of Pb(Ac) 2 instead of lead iodide (PbI 2 ) accelerates the crystal growth and simplifies the fabrication procedure. Even very thin MAPbI 3 films (≈70 nm) can sufficiently support optical amplification and lasing in surface‐emitting distributed‐feedback (DFB) cavities. The facile and highly controllable MAPbI 3 film formation observed with Pb(Ac) 2 as precursor makes it a preferred choice with respect to PbI 2 for future perovskite laser diodes.
    Type of Medium: Online Resource
    ISSN: 1862-6254 , 1862-6270
    URL: Issue
    URL: Article
    DOI: 10.1002/pssr.v13.10
    DOI: 10.1002/pssr.201900176
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2259465-6
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  • 6
    Online Resource
    Online Resource
    Hybrid breeding of rice via genomic selection
    Wiley ; 2020
    In:  Plant Biotechnology Journal Vol. 18, No. 1 ( 2020-01), p. 57-67
    Details
    In: Plant Biotechnology Journal, Wiley, Vol. 18, No. 1 ( 2020-01), p. 57-67
    Abstract: Hybrid breeding is the main strategy for improving productivity in many crops, especially in rice and maize. Genomic hybrid breeding is a technology that uses whole‐genome markers to predict future hybrids. Predicted superior hybrids are then field evaluated and released as new hybrid cultivars after their superior performances are confirmed. This will increase the opportunity of selecting true superior hybrids with minimum costs. Here, we used genomic best linear unbiased prediction to perform hybrid performance prediction using an existing rice population of 1495 hybrids. Replicated 10‐fold cross‐validations showed that the prediction abilities on ten agronomic traits ranged from 0.35 to 0.92. Using the 1495 rice hybrids as a training sample, we predicted six agronomic traits of 100 hybrids derived from half diallel crosses involving 21 parents that are different from the parents of the hybrids in the training sample. The prediction abilities were relatively high, varying from 0.54 (yield) to 0.92 (grain length). We concluded that the current population of 1495 hybrids can be used to predict hybrids from seemingly unrelated parents. Eventually, we used this training population to predict all potential hybrids of cytoplasm male sterile lines from 3000 rice varieties from the 3K Rice Genome Project. Using a breeding index combining 10 traits, we identified the top and bottom 200 predicted hybrids. SNP genotypes of the training population and parameters estimated from this training population are available for general uses and further validation in genomic hybrid prediction of all potential hybrids generated from all varieties of rice.
    Type of Medium: Online Resource
    ISSN: 1467-7644 , 1467-7652
    URL: Issue
    URL: Article
    DOI: 10.1111/pbi.v18.1
    DOI: 10.1111/pbi.13170
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2136367-5
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Growth hormone synergizes with BMP9 in osteogenic differentiation by activating the JAK/STAT/IGF1 pathway in murine multilineage cells
    Wiley ; 2012
    In:  Journal of Bone and Mineral Research Vol. 27, No. 7 ( 2012-07), p. 1566-1575
    Details
    In: Journal of Bone and Mineral Research, Wiley, Vol. 27, No. 7 ( 2012-07), p. 1566-1575
    Type of Medium: Online Resource
    ISSN: 0884-0431
    URL: Article
    DOI: 10.1002/jbmr.1622
    RVK:
    XA 52230
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2008867-X
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  • 8
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    Online Resource
    Characterization of chondrocyte scaffold carriers for cell‐based gene therapy in articular cartilage repair
    Wiley ; 2013
    In:  Journal of Biomedical Materials Research Part A Vol. 101, No. 12 ( 2013-12), p. 3542-3550
    Details
    In: Journal of Biomedical Materials Research Part A, Wiley, Vol. 101, No. 12 ( 2013-12), p. 3542-3550
    Abstract: Articular cartilage lesions in the knee are common injuries. Chondrocyte transplant represents a promising therapeutic modality for articular cartilage injuries. Here, we characterize the viability and transgene expression of articular chondrocytes cultured in three‐dimensional scaffolds provided by four types of carriers. Articular chondrocytes are isolated from rabbit knees and cultured in four types of scaffolds: type I collagen sponge, fibrin glue, hyaluronan, and open‐cell polylactic acid (OPLA). The cultured cells are transduced with adenovirus expressing green fluorescence protein (AdGFP) and luciferase (AdGL3‐Luc). The viability and gene expression in the chondrocytes are determined with fluorescence microscopy and luciferase assay. Cartilage matrix production is assessed by Alcian blue staining. Rabbit articular chondrocytes are effectively infected by AdGFP and exhibited sustained GFP expression. All tested scaffolds support the survival and gene expression of the infected chondrocytes. However, the highest transgene expression is observed in the OPLA carrier. At 4 weeks, Alcian blue‐positive matrix materials are readily detected in OPLA cultures. Thus, our results indicate that, while all tested carriers can support the survival of chondrocytes, OPLA supports the highest transgene expression and is the most conductive scaffold for matrix production, suggesting that OPLA may be a suitable scaffold for cell‐based gene therapy of articular cartilage repairs. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3542–3550, 2013.
    Type of Medium: Online Resource
    ISSN: 1549-3296 , 1552-4965
    URL: Issue
    URL: Article
    DOI: 10.1002/jbm.a.v101.12
    DOI: 10.1002/jbm.a.34661
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 1477192-5
    SSG: 12
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  • 9
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    BACH2‐mediated FOS confers cytarabine resistance via stromal microenvironment alterations in pediatric ALL
    Wiley ; 2021
    In:  Cancer Science Vol. 112, No. 3 ( 2021-03), p. 1235-1250
    Details
    In: Cancer Science, Wiley, Vol. 112, No. 3 ( 2021-03), p. 1235-1250
    Abstract: Acute lymphoblastic leukemia (ALL) is an aggressive hematological cancer that mainly affects children. Relapse and chemoresistance result in treatment failure, underlining the need for improved therapies. BTB and CNC homology 2 (BACH2) is a lymphoid‐specific transcription repressor recognized as a tumor suppressor in lymphomas, but little is known about its function and regulatory network in pediatric ALL (p‐ALL). Herein, we found aberrant BACH2 expression at new diagnosis not only facilitated risk stratification of p‐ALL but also served as a sensitive predictor of early treatment response and clinical outcome. Silencing BACH2 in ALL cells increased cell proliferation and accelerated cell cycle progression. BACH2 blockade also promoted cell adhesion to bone marrow stromal cells and conferred cytarabine (Ara‐C)–resistant properties to leukemia cells by altering stromal microenvironment. Strikingly, we identified FOS , a transcriptional activator competing with BACH2, as a novel downstream target repressed by BACH2. Blocking FOS by chemical compounds enhanced the effect of Ara‐C treatment in both primary p‐ALL cells and pre‐B‐ALL–driven leukemia xenografts and prolonged the survival of tumor‐bearing mice. These data highlight an interconnected network of BACH2‐FOS, disruption of which could render current chemotherapies more effective and offer a promising therapeutic strategy to overcome Ara‐C resistance in p‐ALL.
    Type of Medium: Online Resource
    ISSN: 1347-9032 , 1349-7006
    URL: Issue
    URL: Article
    DOI: 10.1111/cas.v112.3
    DOI: 10.1111/cas.14792
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2115647-5
    detail.hit.zdb_id: 2111204-6
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  • 10
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    Immunohistochemical Localization of Somatostatin in the Brain of Chinese Alligator Alligator sinensis
    Wiley ; 2017
    In:  The Anatomical Record Vol. 300, No. 3 ( 2017-03), p. 507-519
    Details
    In: The Anatomical Record, Wiley, Vol. 300, No. 3 ( 2017-03), p. 507-519
    Abstract: In this study, the regional distribution and histological localization of somatostatin (SS) immunoreactive (IR) perikarya and fibers was investigated for the first time in the brain of adult Chinese alligator by immunohistochemical method. The results showed SS‐IR perikarya and fibers were widely distributed in various parts of the brain except for olfactory bulbs. In the telencephalon, SS‐IR perikarya were predominantly located in the cellular layer and deep plexiform layer of dorsomedial and medial cortex, less in the dorsal and lateral cortex, while SS‐IR fibers were found in all layers of the cerebral cortex. SS‐IR perikarya and fibers were also detected in the dorsal ventricular ridge, hippocampus cortex, accessory olfactory bulb nuclearus, lenticular nucleus, and caudate nucleus. In the diencephalon, SS‐IR perikarya and fibers were mainly present in supraoptic nucleus, paraventricular nucleus of hypothalamus, recessus infundibular nucleus, median eminence, the pineal gland and pituitary gland, in which the IR‐fibers were abundant, appearing dot‐shaped and varicosity‐like. In the mesencephalon, they were present in tectum cortex, ependyma of cerebral aqueduct and the periaqueductal grey matter. Additionally, they were also detected in Purkinje's cellular layer of cerebellum, in the reticularis nucleus and raphe nucleus of medulla oblongata. The distribution pattern of SS‐IR perikarya and fibers in the brain of Chinese alligator is generally similar to that reported in other reptiles, but also has some specific features. The wide distribution indicated that SS might be a neurotransmitter or neuromodulator which acts on many kinds of target cells with a wide range of physiological functions. Anat Rec, 300:507–519, 2017. © 2016 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 1932-8486 , 1932-8494
    URL: Issue
    URL: Article
    DOI: 10.1002/ar.v300.3
    DOI: 10.1002/ar.23474
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2273240-8
    detail.hit.zdb_id: 2109216-3
    SSG: 12
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