In:
Cancer Science, Wiley, Vol. 107, No. 5 ( 2016-05), p. 619-628
Abstract:
An increasingly accepted concept is that the progression of colorectal cancer is accompanied by epithelial–mesenchymal transition ( EMT ). In our study, in order to characterize the properties of EMT in 16 colorectal cancer cell lines, the cells were first orthotopically implanted into nude mice, and the tumors in vivo , as well as cells cultured in vitro , were immunostained for EMT markers. The immunostaining revealed that seven of the cells had an epithelial phenotype with a high expression of E‐cadherin, whereas other cells showed opposite patterns, such as a high expression of vimentin ( CX ‐1, COLO 205, CloneA, HCT 116, and SW 48). Among the cells expressing vimentin, some expressed vimentin in the orthotopic tumors but not in the cultured cells ( SW 480, SW 620, and COLO 320). We evaluated these findings in combination with microarray analyses, and selected five genes: CHST 11, SERPINI 1 , AGR 2, FBP 1 , and FOXA 1 . Next, we downregulated the expression of SERPINI 1 with si RNA in the cells, the results of which showed reverse‐ EMT changes at the protein level and in the cellular morphology. Along with immunohistochemical analyses, we confirmed the effect of the intracellular and secreted SERPINI 1 protein of SW 620 cells, which supported the importance of SERPINI 1 in EMT . The development of therapeutic strategies targeting EMT is ongoing, including methods targeting the transforming growth factor‐β signaling pathway as well as the Wnt pathway. SERPINI 1 is an important regulator of EMT . Our findings help to elucidate the signaling pathways of EMT , hopefully clarifying therapeutic pathways as well.
Type of Medium:
Online Resource
ISSN:
1347-9032
,
1349-7006
DOI:
10.1111/cas.2016.107.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2115647-5
detail.hit.zdb_id:
2111204-6
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