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  • 1
    Online Resource
    Online Resource
    Pulsed microwave exposure real time and dynamically reduces reactive oxygen species in rat pheochromocytoma cells in vivo
    Wiley ; 2015
    In:  Microwave and Optical Technology Letters Vol. 57, No. 10 ( 2015-10), p. 2395-2400
    Details
    In: Microwave and Optical Technology Letters, Wiley, Vol. 57, No. 10 ( 2015-10), p. 2395-2400
    Abstract: With the widespread and ever‐increasing application of microwave technology in our daily lives, the potential adverse effects on biological systems from exposure to microwaves are extremely interesting based on thermal and nonthermal effects, especially nonthermal effects on the nervous system. To real time and dynamically elucidate the diversity of reactive oxygen species (ROS) in rat pheochromocytoma (PC12) cells using a real‐time‐pulsed microwave device combining laser confocal microscopy (LSCM). A LSCM system was designed, and its physical properties, including irradiation cavity simulation, field intensity distribution, and average specific absorption rate (SAR), were analyzed and a stable field intensity distribution as well as a precise average SAR were observed. Subsequently, the diversity of ROS in PC12 cells under pulsed microwave exposure was determined by LSCM online. Additionally, the total ROS in PC12 cells was determined by flow cytometry after pulsed microwave exposure. The ROS signal in single PC12 cell was rapidly quenched as the irradiation time was extended, whereas the total ROS showed no changes in flow cytometry after microwave exposure. This indicates that the change of intracellular ROS in PC12 cells by microwave exposure is a real‐time and dynamic process, and provides a significant reference for studying the biological effects of microwaves. © 2015 Wiley Periodicals, Inc. Microwave Opt Technol Lett 57:2395–2400, 2015
    Type of Medium: Online Resource
    ISSN: 0895-2477 , 1098-2760
    URL: Issue
    URL: Article
    DOI: 10.1002/mop.v57.10
    DOI: 10.1002/mop.29342
    Language: English
    Publisher: Wiley
    Publication Date: 2015
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  • 2
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    Online Resource
    Abnormality of synaptic vesicular associated proteins in cerebral cortex and hippocampus after microwave exposure
    Wiley ; 2009
    In:  Synapse Vol. 63, No. 11 ( 2009-11), p. 1010-1016
    Details
    In: Synapse, Wiley, Vol. 63, No. 11 ( 2009-11), p. 1010-1016
    Abstract: Studies were performed to determine the effects of microwave on synaptic vesicles and the expression of synaptic vesicular associated proteins including synapsin I, VAMP‐2, syntaxin, and synaptophysin. 25 Wistar rats were exposed to microwave which the average power density was 30 mW/cm 2 , and whole body average specific absorption rate was 14.1 W/kg for 5 min. Synaptosome preparations in the cerebral cortex and hippocampus were obtained by isotonic Percoll/sucrose discontinuous gradients at 6 h, 1, 3, and 7 days after radiation. The expression of synaptic vesicular associated proteins was measured using Western blots and image analysis. The interaction between VAMP‐2 and syntaxin was examined by coimmunoprecipitation analysis. Synapsin I in the cerebral cortex were decreased at 3 days ( P 〈 0.01) after radiation and in the hippocampus increased at 1 day ( P 〈 0.01), decreased at 3 days ( P 〈 0.01), increased again at 7 days ( P 〈 0.01) after exposure, compared with the sham‐treated controls. Synaptophysin were increased in 1–7 days ( P 〈 0.01) after exposure in the cerebral cortex and hippocampus. VAMP‐2 were decreased at 1 and 3 days ( P 〈 0.01) and syntaxin were decreased in 6 h to 3 days ( P 〈 0.01) after radiation in the cerebral cortex and hippocampus. The interactions between VAMP‐2 and syntaxin were decreased at 3–7 days ( P 〈 0.01) after radiation in the cerebral cortex and hippocampus, compared with the sham‐treated controls. These results suggest that 30 mW/cm 2 (SAR 14.1 W/kg) microwave radiation can result in the perturbation of the synaptic vesicles associated proteins: synapsin I, synaptophysin, VAMP‐2, and syntaxin. The perturbation could induce the deposit of synaptic vesicle, which might be relative to the dysfunction of the synaptic transmission, even the cognition deficit. Synapse 63:1010–1016, 2009. © 2009 Wiley‐Liss, Inc.
    Type of Medium: Online Resource
    ISSN: 0887-4476 , 1098-2396
    URL: Issue
    URL: Article
    DOI: 10.1002/syn.v63:11
    DOI: 10.1002/syn.20684
    Language: English
    Publisher: Wiley
    Publication Date: 2009
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    SSG: 12
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  • 3
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    Submicron SiO 2 Powder: Characterization and Effects on Properties of Cement‐Free Iron Ditch Castables
    Wiley ; 2021
    In:  Advances in Materials Science and Engineering Vol. 2021, No. 1 ( 2021-01)
    Details
    In: Advances in Materials Science and Engineering, Wiley, Vol. 2021, No. 1 ( 2021-01)
    Abstract: Submicron materials are those with particle size diameters between 0.1 and 1  μ m. Submicron SiO 2 generally refers to SiO 2 powder with a D 90   〈  1  μ m ( D 90 refers to the particle size distribution exhibited by the sample and corresponds, in this case, to 90% of the particles not exceeding a diameter of 1  μ m). In this study, a new type of cement‐free iron ditch castable was prepared using dense corundum and silicon carbide as the primary raw materials with submicron SiO 2 powder as the binder. The effects of submicron SiO 2 powder content on the bulk density, apparent porosity, linear rate of change, compressive strength, and bending strength were investigated. The mechanism of action of the submicron SiO 2 powder was also investigated by analyzing its microstructure and particle size distribution. The results revealed that (1) the submicron SiO 2 powder can be used as the sole bonding agent in the preparation of cement‐free iron ditch castables; (2) in comparison to traditional castables, the cement‐free castable developed in this study demonstrated strong service performance and high‐temperature bending strength.
    Type of Medium: Online Resource
    ISSN: 1687-8434 , 1687-8442
    URL: Issue
    URL: Article
    DOI: 10.1155/amse.v2021.1
    DOI: 10.1155/2021/9385917
    Language: English
    Publisher: Wiley
    Publication Date: 2021
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  • 4
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    Online Resource
    Selective Covalent Targeting of Anti‐apoptotic BFL‐1 by a Sulfonium‐Tethered Peptide
    Wiley ; 2021
    In:  ChemBioChem Vol. 22, No. 2 ( 2021-01-15), p. 340-344
    Details
    In: ChemBioChem, Wiley, Vol. 22, No. 2 ( 2021-01-15), p. 340-344
    Abstract: Anti‐apoptotic B cell lymphoma 2 (BCL‐2) family proteins are proven targets for human cancers. Targeting the BH3‐binding pockets of these anti‐apoptotic proteins could reactivate apoptosis in BCL‐2‐depedent cancers. BFL‐1 is a BCL‐2 family protein overexpressed in various chemoresistant cancers. A unique cysteine at the binding interface of the BH3 and BFL‐1 was previously proven to be an intriguing targeting site to irreversibly inhibit BFL‐1 functions with stabilized cyclic peptide bearing a covalent warhead. Recently, we developed a sulfonium‐tethered peptide cyclization strategy to construct peptide ligands that could selectively and efficiently react with the cysteine(s) of target proteins near the interacting interface. Using this method, we constructed a BFL‐1 peptide inhibitor, B4‐MC, that could selectively conjugate with BFL‐1 both in vitro and in cell. B4‐MC showed good cellular uptake, colocalized with BFL‐1 on mitochondria, and showed obvious growth inhibition of BFL‐1 over‐expressed cancer cell lines.
    Type of Medium: Online Resource
    ISSN: 1439-4227 , 1439-7633
    URL: Issue
    URL: Article
    DOI: 10.1002/cbic.v22.2
    DOI: 10.1002/cbic.202000473
    RVK:
    VA 2918
    Language: English
    Publisher: Wiley
    Publication Date: 2021
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    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities
    Wiley ; 2016
    In:  Oxidative Medicine and Cellular Longevity Vol. 2016, No. 1 ( 2016-01)
    Details
    In: Oxidative Medicine and Cellular Longevity, Wiley, Vol. 2016, No. 1 ( 2016-01)
    Abstract: Since excessive reactive oxygen species (ROS) is known to be associated with aging and age‐related diseases, strategies modulating ROS level and antioxidant defense systems may contribute to the delay of senescence. Here we show that the protein hydrolyzate from Angelica sinensis was capable of increasing oxidative survival of the model animal Caenorhabditis elegans intoxicated by paraquat. The hydrolyzate was then fractionated by ultrafiltration, and the antioxidant fraction ( 〈 3 kDa) was purified by gel filtration to obtain the antioxidant A. sinensis peptides (AsiPeps), which were mostly composed of peptides with 〈 20 amino acid residues. Further studies demonstrate that AsiPeps were able to reduce the endogenous ROS level, increase the activities of the antioxidant enzymes superoxide dismutase and catalase, and decrease the content of the lipid peroxidation product malondialdehyde in nematodes treated with paraquat or undergoing senescence. AsiPeps were also shown to reduce age pigments accumulation and extend lifespan but did not affect the food‐intake behavior of the nematodes. Taken together, our results demonstrate that A. sinensis peptides (AsiPeps) are able to delay aging process in C. elegans through antioxidant activities independent of dietary restriction.
    Type of Medium: Online Resource
    ISSN: 1942-0900 , 1942-0994
    URL: Issue
    URL: Article
    DOI: 10.1155/omcl.v2016.1
    DOI: 10.1155/2016/8956981
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2455981-7
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