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  • 1
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    The potential diagnosis role of TP53 mutation in advanced bladder cancer: A meta‐analysis
    Wiley ; 2021
    In:  Journal of Clinical Laboratory Analysis Vol. 35, No. 5 ( 2021-05)
    Details
    In: Journal of Clinical Laboratory Analysis, Wiley, Vol. 35, No. 5 ( 2021-05)
    Abstract: Bladder cancer is one of the most common urological cancers all over the world, and NMIBC occupies almost 80% of recently diagnosed bladder cancer cases. Progress and recurrence of bladder cancer are the main problems during the disease. The level of TP53 mutation is obviously higher in the high stage than the lower. This meta‐analysis is to evaluate the potential diagnosis feature of TP53 mutation by the expression of TP53 mutation of Ta stage vs high stage in bladder cancer. Methods A systematic search of databases was conducted, and some relevant articles were selected. Next, the meta‐analysis was carried out according to the standard guidelines. Results There were seven researches in which 677 participants were selected at the basis of inclusion standard. TP53 mutation was associated highly with increased diagnosis of bladder cancer. We found that the high stage of bladder cancer has obviously higher level of TP53 mutation than the lower stage, and these patients of MIBC have higher expression of TP53 mutation compared with NMIBC. No significant publication bias has been observed in this meta‐analysis. The expression of TP53 mutation might be a diagnose‐related biomarker for lots of patients with bladder cancer. Conclusions The results of this meta‐analysis provided further evidences that the expression of TP53 mutation was associated with the diagnosis efficiency of advanced bladder cancer. Higher expression of TP53 mutation was observed in the high stage of bladder cancer or the MIBC, and lower expression of TP53 mutation in the Ta stage of bladder cancer or the NMIBC. The expression level of TP53 mutation was probably a critical diagnosed biomarker in advanced bladder cancer.
    Type of Medium: Online Resource
    ISSN: 0887-8013 , 1098-2825
    URL: Issue
    URL: Article
    DOI: 10.1002/jcla.v35.5
    DOI: 10.1002/jcla.23765
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2001635-9
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  • 2
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    Cross‐platform comparison of next‐generation sequencing and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for detecting KRAS/NRAS/BRAF/PIK3CA mutations in cfDNA from metastatic colorectal cancer patients
    Wiley ; 2021
    In:  Journal of Clinical Laboratory Analysis Vol. 35, No. 9 ( 2021-09)
    Details
    In: Journal of Clinical Laboratory Analysis, Wiley, Vol. 35, No. 9 ( 2021-09)
    Abstract: Examining tumor KRAS/NRAS/BRAF/PIK3CA status in metastatic colorectal cancer (mCRC) is essential for treatment selection and prognosis evaluation. Cell‐free DNA (cfDNA) in plasma is a feasible source for tumor gene analysis. Methods In this study, we recruited mCRC patients and analyzed their KRAS/NRAS/BRAF/PIK3CA status in cfDNA using two platforms, next‐generation sequencing (NGS) and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF). The performance between the two platforms and the concordance rate between cfDNA and tissue were analyzed. The relationship between cfDNA‐related variables and clinical variables was also assessed. Tumor mutations in cfDNA from patients receiving continuous treatments were monitored in the follow‐ups. Results Next‐generation sequencing and MALDI‐TOF had similar specificity (100.0% vs. 99.3%) and negative predictive value (99.9% vs. 99.4%), whereas NGS had higher sensitivity (97.1% vs. 85.3% of MALDI‐TOF) and positive predictive value (100% vs. 82.9% of MALDI‐TOF). The overall concordance rate of NGS and MALDI‐TOF was 98.6%. For the reportable types of mutations in both cfDNA and tissue, the concordance rate was 96.1%. Among 28 tissue‐positive patients, the allele frequencies of tumor mutations in cfDNA were higher in patients with primary tumor burden ( p  = 0.0141). Both CEA and CA 19‐9 were positively correlated with cfDNA concentration ( r  = 0.3278 and r  = 0.3992). The allele frequencies of tumor mutations changed with disease progression. Conclusions Next‐generation sequencing showed slightly better performance in detecting cfDNA mutations and was more suitable for clinical practice. cfDNA‐related variables reflected the tumor status and showed a promising potential in monitoring disease progression.
    Type of Medium: Online Resource
    ISSN: 0887-8013 , 1098-2825
    URL: Issue
    URL: Article
    DOI: 10.1002/jcla.v35.9
    DOI: 10.1002/jcla.23818
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2001635-9
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  • 3
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    Rapid and label‐free identification of different cancer types based on surface‐enhanced Raman scattering profiles and multivariate statistical analysis
    Wiley ; 2021
    In:  Journal of Cellular Biochemistry Vol. 122, No. 2 ( 2021-02), p. 277-289
    Details
    In: Journal of Cellular Biochemistry, Wiley, Vol. 122, No. 2 ( 2021-02), p. 277-289
    Abstract: Rapid detection and classification of cancer cells with label‐free and non‐destructive methods are helpful for rapid screening of cancer patients in clinical settings. Here, surface‐enhanced Raman scattering (SERS) was used for rapid, unlabeled, and non‐destructive detection of seven different cell types, including human cancer cells and non‐tumorous cells. Au nanoparticles were used as enhanced substrates and directly added to cell surfaces. The single cellular SERS signals could be easily and stably collected in several minutes, and the cells maintained structural integrity over one hour. Different types of cells had unique Raman phenotypes. By applying multivariate statistical analysis to the Raman phenotypes, the cancer cells and non‐tumorous cells were accurately identified. The high sensitivity enabled this method to discriminate subtle molecular changes in different cell types, and the accuracy reached 81.2% with principal components analysis and linear discriminant analysis. The technique provided a rapid, unlabeled, and non‐destructive method for the detection and identification of various cancer types.
    Type of Medium: Online Resource
    ISSN: 0730-2312 , 1097-4644
    URL: Issue
    URL: Article
    DOI: 10.1002/jcb.v122.2
    DOI: 10.1002/jcb.29857
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 1479976-5
    SSG: 12
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  • 4
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    Mo 3 S 13 2− Intercalated Layered Double Hydroxide: Highly Selective Removal of Heavy Metals and Simultaneous Reduction of Ag + Ions to Metallic Ag 0 Ribbons
    Wiley ; 2022
    In:  Angewandte Chemie International Edition Vol. 61, No. 1 ( 2022-01-03)
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 61, No. 1 ( 2022-01-03)
    Abstract: We demonstrate a new material by intercalating Mo 3 S 13 2− into Mg/Al layered double hydroxide ( abbr . Mo 3 S 13 ‐LDH), exhibiting excellent capture capability for toxic Hg 2+ and noble metal silver (Ag). The as‐prepared Mo 3 S 13 ‐LDH displays ultra‐high selectivity of Ag + , Hg 2+ and Cu 2+ in the presence of various competitive ions, with the order of Ag + 〉 Hg 2+ 〉 Cu 2+ 〉 Pb 2+ ≥Co 2+ , Ni 2+ , Zn 2+ , Cd 2+ . For Ag + and Hg 2+ , extremely fast adsorption rates (≈90 % within 10 min, 〉 99 % in 1 h) are observed. Much high selectivity is present for Ag + and Cu 2+ , especially for trace amounts of Ag + (≈1 ppm), achieving a large separation factor (SF Ag/Cu ) of ≈8000 at the large Cu/Ag ratio of 520. The overwhelming adsorption capacities for Ag + ( q m Ag =1073 mg g −1 ) and Hg 2+ ( q m Hg =594 mg g −1 ) place the Mo 3 S 13 ‐LDH at the top of performing sorbent materials. Most importantly, Mo 3 S 13 ‐LDH captures Ag + via two paths: a) formation of Ag 2 S due to Ag‐S complexation and precipitation, and b) reduction of Ag + to metallic silver (Ag 0 ). The Mo 3 S 13 ‐LDH is a promising material to extract low‐grade silver from copper‐rich minerals and trap highly toxic Hg 2+ from polluted water.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v61.1
    DOI: 10.1002/anie.202112511
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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  • 5
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    Estrogen reverses nicotine‐induced inflammation in chondrocytes via reducing the degradation of ECM
    Wiley ; 2019
    In:  International Journal of Rheumatic Diseases Vol. 22, No. 4 ( 2019-04), p. 666-676
    Details
    In: International Journal of Rheumatic Diseases, Wiley, Vol. 22, No. 4 ( 2019-04), p. 666-676
    Abstract: Osteoarthritis (OA) is a chronic disease with a very high incidence and the pathology of which is quite complex. Epidemiological investigation showed that OA may be related to smoking and estrogen levels, but there are few studies focused on the cross‐effect of these two factors. This research aims to investigate the molecular mechanism of nicotine and estrogen effects on chondrocytes to study the effect of smoking on the incidence of osteoarthritis in women. Method of the study Nicotine was added to obtain inflammatory supernatants of macrophages, which were used to induce chondrocyte inflammation. Toluidine staining and immunohistochemistry were used to detect the extracellular matrix (ECM) of chondrocytes, while the important proteins in the metabolism of chondrocytes were detected by Western blot. Results Nicotine‐induced inflammatory supernatant promoted the degradation of ECM, such as type II collagen, aggrecan and proteoglycan 4. While in the presence of physiological concentrations of estrogen, this destructive effect is reversed. On the molecular level, estrogen (17β ‐ estradiol, 1 nmol/L) can inhibit the matrix degrading enzymes and promote the transforming growth factor (TGF)‐β1 pathway which is involved in matrix synthesis. However, in the presence of inflammatory induction, although estrogen could still inhibit the expression of matrix degrading enzymes, it inhibited the TGF‐β1 pathway. Moreover, the different inflammatory factors in the inflammatory supernatant, mainly tumor necrosis factor‐α, interleukin‐1β, had different effects on the metabolic processes of chondrocytes. Conclusion Estrogen reverses nicotine‐induced inflammation mainly via reducing the degradation of ECM. The cross‐effect of estrogen and inflammatory factor inhibitors can be a potential clinical reference for OA patients.
    Type of Medium: Online Resource
    ISSN: 1756-1841 , 1756-185X
    URL: Issue
    URL: Article
    DOI: 10.1111/apl.2019.22.issue-4
    DOI: 10.1111/1756-185X.13476
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2427877-4
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  • 6
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    Thrombosis among 1537 patients with JAK2 V617F ‐mutated myeloproliferative neoplasms: Risk factors and development of a predictive model
    Wiley ; 2020
    In:  Cancer Medicine Vol. 9, No. 6 ( 2020-03), p. 2096-2105
    Details
    In: Cancer Medicine, Wiley, Vol. 9, No. 6 ( 2020-03), p. 2096-2105
    Abstract: To explore the risk factors of thrombosis in patients with JAK2 V617F ‐ mutated myeloproliferative neoplasms (MPNs), a cohort of 1537 Chinese patients with JAK2 V617F ‐mutated MPN was retrospectively analyzed. The Kaplan‐Meier method and multivariate Cox analysis were used to study the risk factors of thrombosis in patients with JAK2 V617F ‐mutated MPN. Among the 1537 MPN patients, 931, 468, and 138 had polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), respectively. The median follow‐up time was 7 years (range 1‐47), and 12.8% of patients (197/1537) died during this period. A total of 16.8% (259/1399) of PV and ET patients had secondary myelofibrosis, and 2.5% (38/1537) of patients developed acute myeloid leukemia (AML). Thrombotic events occurred in 43.9% (675/1537) of patients, among which 91.4% (617/675) were arterial thrombosis and 16.6% (112/675) were venous thrombosis. The number of thrombotic events in PV, ET, and PMF patients was 439 (47.2%), 197 (42.1%) and 39 (28.2%), respectively. The multivariate analysis indicated that age ≥60 years old, HCT ≥48%, at least one cardiovascular risk factor, a history of thrombosis, and JAK2 V617F allele burden ( V617F %) ≥50% are risk factors for thrombosis in JAK2 V617F ‐mutated MPN. According to the results of the multivariate analysis, a risk model of thrombosis was established and comprised low‐risk (0 points), intermediate‐risk (1 points) and high‐risk (≥2 points) groups, among which the incidence of thrombosis was 9.1%, 33.7% and 72.9%. For elderly patients with JAK2 V617F ‐mutated MPN and a history of thrombosis, reducing the V617F% , controlling HCT and preventing cardiovascular risk factors are necessary measures to prevent thrombosis.
    Type of Medium: Online Resource
    ISSN: 2045-7634 , 2045-7634
    URL: Issue
    URL: Article
    DOI: 10.1002/cam4.v9.6
    DOI: 10.1002/cam4.2886
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2659751-2
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  • 7
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    Sulfate Modification and Evaluation of in vitro Anti‐HIV Activity of Lycium Barbarum Polysaccharides
    Wiley ; 2022
    In:  ChemistrySelect Vol. 7, No. 37 ( 2022-10-07)
    Details
    In: ChemistrySelect, Wiley, Vol. 7, No. 37 ( 2022-10-07)
    Abstract: This study investigated the feasibility of sulfate modification of Lycium barbarum polysaccharides (LBPs) and their potential anti‐HIV‐1 application. LBPs with different molecular weights were prepared by fractional precipitation from crude LBP via aqueous extraction, ethanol precipitation, and deproteinization. The purified LBPs (G1, G2, and G3) were sulfated using the chlorosulfonic acid pyridine method to obtain sulfated LBPs (G1S1–G1S4, G2S1–G2S4, and G3S1–G3S4) with different sulfur degrees of substitution. The anti‐HIV‐1 activity in vitro was evaluated via the TD 50 from the cytotoxicity test and the IC 50 inhibitory activity. The cytotoxicity and inhibitory activity in vitro were carried out using the CCK‐8 assay and MAGI test with Azidothymidine (AZT) as a positive control. The results show that the LBPs and the sulfated derivatives were not toxic towards CCK‐8 cells with TD 50 〉 100 μg/mL and they had strong anti‐HIV‐1 activity with IC 50 values of 0.02–0.12 μg/mL. Compared with the LBPs, the anti‐HIV‐1 activity of the sulfated LBPs increased with IC 50 values from 0.0924–0.1206 μg/mL to 0.0206–0.0722 μg/mL. Sulfate modification of LBPs can increase their anti‐HIV pharmacological activity, the G1S4 ( M w=2.13×10 4  Da and DS=1.12) showed relatively good in vitro anti‐HIV‐1 activity with an IC 50 value near that of AZT (0.0200 μg/mL). The results suggest further detailed in vivo studies are warranted for these promising candidates with anti‐HIV‐1 activity.
    Type of Medium: Online Resource
    ISSN: 2365-6549 , 2365-6549
    URL: Issue
    URL: Article
    DOI: 10.1002/slct.v7.37
    DOI: 10.1002/slct.202200695
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2844262-3
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  • 8
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    The repeated administration of rhIL‐12 for 14 weeks in rhesus monkeys: A toxicity assessment
    Wiley ; 2023
    In:  Journal of Applied Toxicology
    Details
    In: Journal of Applied Toxicology, Wiley
    Abstract: This study found that repeated administration of recombinant human interleukin 12 (rhIL‐12) in rhesus monkeys revealed dose‐dependent toxicity. The low dose (0.5 μg/kg) showed no adverse effects, while higher doses (up to 7.5 μg/kg) resulted in symptoms such as loose stools, reduced activity, anemia, and liver function abnormalities. The No Observed Adverse Effect Level (NOAEL) was determined to be 0.5 μg/kg, and the Highest Non Severely Toxic Dose (HNSTD) was 7.5 μg/kg.
    Type of Medium: Online Resource
    ISSN: 0260-437X , 1099-1263
    URL: Article
    DOI: 10.1002/jat.4541
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1475015-6
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  • 9
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    Clinical features and mutation of NPHP5 in two C hinese siblings with S enior‐ L øken syndrome
    Wiley ; 2013
    In:  Nephrology Vol. 18, No. 12 ( 2013-12), p. 838-842
    Details
    In: Nephrology, Wiley, Vol. 18, No. 12 ( 2013-12), p. 838-842
    Abstract: S enior‐ L øken syndrome is a rare syndromic form of nephronophthisis that is associated with retinal dystrophy. Presently, seven genes ( NPHP1 ‐6 and NPHP10 ) have been associated with S enior‐ L øken syndrome. NPHP5 mutations are known to cause classical S enior‐ L øken syndrome. Here, we report two sisters ( II ‐4, II ‐5) from a C hinese H an ethnic family who presented with classical S enior‐ L øken syndrome. Both affected sisters exhibited L eber's congenital amaurosis and juvenile nephronophthisis that progressed to end‐stage renal disease by the age of 16 years and 9 months in patient II ‐4 and 12 years and 9 months in patient II ‐5. Sequence analysis showed a homozygous truncated mutation in NPHP5 , c.1090 C 〉 T (p. R364X ), in the patient II ‐4. This mutation is predicted to introduce a new open reading frame that results in the truncation of the C ‐terminal 235 amino acids of nephrocystin‐5 and its consequent loss of function. Both parents carried a single heterozygous mutation in the same position, and no homozygous deletion of NPHP1 was found in this pedigree.
    Type of Medium: Online Resource
    ISSN: 1320-5358 , 1440-1797
    URL: Issue
    URL: Article
    DOI: 10.1111/nep.2013.18.issue-12
    DOI: 10.1111/nep.12156
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2008235-6
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  • 10
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    Mitochondria‐Targeted Small‐Molecule Fluorophores for Dual Modal Cancer Phototherapy
    Wiley ; 2016
    In:  Advanced Functional Materials Vol. 26, No. 17 ( 2016-05), p. 2826-2835
    Details
    In: Advanced Functional Materials, Wiley, Vol. 26, No. 17 ( 2016-05), p. 2826-2835
    Abstract: Mitochondria are recognized as the ideal target for cancer treatment because they play a central role in oxidative metabolism and apoptosis. In this work, a mitochondria‐targeted near‐infrared (NIR) photosensitizer (PS) for synchronous cancer photodynamic therapy (PDT) and photothermal therapy (PTT) is synthesized. This multifunctional small‐molecule PS is developed from a variety of synthesized heptamethine cyanine dyes, which are modified with various N ‐alkyl side chains on the lipophilic cationic heptamethine core. It is demonstrated to preferentially accumulate in cancer cells by organic‐anion transporting polypeptide mediated active transport and retain in mitochondria by its lipophilic cationic property. As mitochondria are susceptible to hyperthermia and excessive reactive oxygen species, this new PS integrating PTT and PDT treatment exhibits highly efficient phototherapy in multiple cancer cells and animal xenograft models. Furthermore, this targeted PS with NIR imaging property also enables tumors and their margins clearly visualized, providing the potential for precisely imaging‐guided phototherapy and treatment monitoring. This is the first report that a small‐molecule PS integrates both cancer PTT and PDT treatment by targeting mitochondria, significantly increasing the photosensitization. This work may also present a practicable strategy to develop small‐molecule‐based cancer theranostic agents for simultaneous cancer targeting, imaging, and therapy.
    Type of Medium: Online Resource
    ISSN: 1616-301X , 1616-3028
    URL: Issue
    URL: Article
    DOI: 10.1002/adfm.v26.17
    DOI: 10.1002/adfm.201600159
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2029061-5
    detail.hit.zdb_id: 2039420-2
    SSG: 11
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