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  • 1
    In: Journal of Neurochemistry, Wiley, Vol. 128, No. 5 ( 2014-03), p. 686-700
    Abstract: The WWC 1 gene has been genetically associated with human episodic memory performance, and its product KIdney/BRAin protein ( KIBRA ) has been shown to interact with the atypical protein kinase protein kinase M ζ (PKMζ). Although recently challenged, PKM ζ remains a candidate postsynaptic regulator of memory maintenance. Here, we show that PKM ζ is subject to rapid proteasomal degradation and that KIBRA is both necessary and sufficient to counteract this process, thus stabilizing the kinase and maintaining its function for a prolonged time. We define the binding sequence on KIBRA , a short amino acid motif near the C‐terminus. Both hippocampal knock‐down of KIBRA in rats and KIBRA knock‐out in mice result in decreased learning and memory performance in spatial memory tasks supporting the notion that KIBRA is a player in episodic memory. Interestingly, decreased memory performance is accompanied by decreased PKM ζ protein levels. We speculate that the stabilization of synaptic PKM ζ protein levels by KIBRA may be one mechanism by which KIBRA acts in memory maintenance. image KIBRA/WWC1 has been genetically associated with human episodic memory. KIBRA has been shown to be post‐synaptically localized, but its function remained obscure. Here, we show that KIBRA shields PKMζ, a kinase previously linked to memory maintenance, from proteasomal degradation via direct interaction. KIBRA levels in the rodent hippocampus correlate closely both to spatial memory performance in rodents and to PKMζ levels. Our findings support a role for KIBRA in memory, and unveil a novel function for this protein.
    Type of Medium: Online Resource
    ISSN: 0022-3042 , 1471-4159
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2014
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  • 2
    In: Advanced Functional Materials, Wiley, Vol. 28, No. 30 ( 2018-07)
    Abstract: In the field of flexible electronics, emerging applications require biocompatible and unobtrusive devices, which can withstand different modes of mechanical deformation and achieve low complexity in the fabrication process. Here, the fabrication of a mesa‐shaped elastomeric substrate, supporting thin‐film transistors (TFTs) and logic circuits (inverters), is reported. High‐relief structures are designed to minimize the strain experienced by the electronics, which are fabricated directly on the pillars' surface. In this design configuration, devices based on amorphous indium‐gallium‐zinc‐oxide can withstand different modes of deformation. Bending, stretching, and twisting experiments up to 6 mm radius, 20% uniaxial strain, and 180° global twisting, respectively, are performed to show stable electrical performance of the TFTs. Similarly, a fully integrated digital inverter is tested while stretched up to 20% elongation. As a proof of the versatility of mesa‐shaped geometry, a biocompatible and stretchable sensor for temperature mapping is also realized. Using pectin, which is a temperature‐sensitive material present in plant cells, the response of the sensor shows current modulation from 13 to 28 °C and functionality up to 15% strain. These results demonstrate the performance of highly flexible electronics for a broad variety of applications, including smart skin and health monitoring.
    Type of Medium: Online Resource
    ISSN: 1616-301X , 1616-3028
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2029061-5
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  • 3
    Online Resource
    Online Resource
    Wiley ; 2013
    In:  Progress in Photovoltaics: Research and Applications Vol. 21, No. 4 ( 2013-06), p. 681-692
    In: Progress in Photovoltaics: Research and Applications, Wiley, Vol. 21, No. 4 ( 2013-06), p. 681-692
    Abstract: We report on the optimization of selective picosecond laser structuring for the monolithic serial interconnection of (Cu(In,Ga)(S,Se) 2 ) CIS thin film solar cells. We introduce a quantitative value to compare the energy efficiency of the different investigated laser processes, the specific ablation energy, which indicates the required energy to remove a certain volume of the specific material. We have examined the structuring efficiencies for induced laser ablation processes for a modification of the beam profile (elliptical and flat‐top beam shaping) and for the application of different laser wavelengths (1064 and 532 nm). Application of induced laser processes (often referred as “lift‐off”) decreases the specific ablation energy dramatically by nearly one order of magnitude. Modifications of the beam profile such as elliptical and flat‐top beam shaping are nearly halving the energy per ablated volume relative to a circular beam. The application of a laser wavelength 532 nm decreases the specific ablation energy compared with 1064 nm significantly for processes involving the CIS layer. We finally demonstrate that with a picosecond laser power of only 2 W, the molybdenum back contact (P1, glass side) and the ZnO front contact (P3, ZnO on CIS) can be structured with a process speed of up to 4 m/s. About 2 µm thick CIS layer (P2) is structured by standard direct laser ablation at higher energy densities with 200 mm/s. Copyright © 2012 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 1062-7995 , 1099-159X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2013
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  • 4
    In: Progress in Photovoltaics: Research and Applications, Wiley, Vol. 23, No. 10 ( 2015-10), p. 1291-1304
    Abstract: In this paper, we present the selective structuring of all three patterns (P1, P2 and P3) of a monolithic interconnection of CIS (Cu(In,Ga)(S,Se) 2 ) thin film solar cells by picosecond laser pulses at a wavelength of 1064 nm. We show results for single pulse ablation threshold values and line scribing of molybdenum films on glass (P1), CIS on molybdenum (P2) and zinc oxide on CIS (P3). The purposes of these processes are the p‐type isolation (P1), cell interconnect (P2) and n‐type isolation (P3), which are required for complete cell architecture. The half micron thick molybdenum back electrode can be structured with a process speed of more than 15 m/s at about 15 W average power without detectable residues and damage by direct induced laser ablation from the back side (P1). The CIS layer can be structured selectively down to the molybdenum at process speeds up to 1 m/s at about 15 W average power, due to the precision of direct laser ablation in the ultrashort pulse regime (P2). The ZnO front electrode layer is separated by clean trenches with straight side walls at process speeds of up to 15 m/s at about 10 W average power, as a result of indirect induced laser ablation (P3). A validation of functionality of all processes is demonstrated on CIS solar cell modules (30 × 30 cm 2 ). By replacing one state‐of‐the‐art process by a picosecond laser process at a time, solar efficiencies could be increased for P1 and P2 and stayed on a similar level for P3. After an optimization of the patterning processes in the R & D pilot line of AVANCIS, we achieved a new record efficiency for an all‐laser‐patterned CIS solar module: 14.7% as best value for the aperture area efficiency of a 30 × 30 cm 2 sized CIS module was reached. Copyright © 2014 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 1062-7995 , 1099-159X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2023295-0
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  • 5
    Online Resource
    Online Resource
    Wiley ; 2022
    In:  Bautechnik Vol. 99, No. 6 ( 2022-06), p. 463-470
    In: Bautechnik, Wiley, Vol. 99, No. 6 ( 2022-06), p. 463-470
    Abstract: Digital twin for the automated production of precast concrete elements Using precast concrete elements in construction has many advantages, such as cost and planning reliability, precision, and efficiency. In most cases, the production of precast concrete elements is not fully automated. There are many manual work steps that prevent a further increase in productivity. The basis for full automation is the use of information and communication technology. A digital twin as an integrated data model is necessary for this. This paper examines how the combination of Building Information Modeling with methods from the context of Industry 4.0, which enable largely self‐organized and decentralized production, can provide the basis for the complete automation and networking of all production steps. Based on the administration shell, which implements the digital twin in Industry 4.0, a suitable description for digital twins of precast concrete modules is being developed by developing data and interaction models for industrialized production.
    Type of Medium: Online Resource
    ISSN: 0932-8351 , 1437-0999
    URL: Issue
    RVK:
    Language: German
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2151238-3
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  • 6
    In: Liver International, Wiley, Vol. 32, No. 9 ( 2012-10), p. 1354-1362
    Abstract: Glycolipids have been shown to serve specialized functions in cell signalling, proliferation and differentiation processes, which are all important during liver regeneration. We previously generated beta‐glucosidase 2 ( GBA 2) knockout mice that accumulate the glycolipid glucosylceramide in various tissues, including the liver. The present study addressed the role of GBA 2‐deficiency and subsequent glucosylceramide accumulation in liver regeneration. Methods Gba2 knockout and wild‐type mice were subjected to two‐third partial hepatectomy. Mice were sacrificed at different time points, blood was collected, and the remnant liver was removed. Glucosylceramide and ceramide were quantified using mass spectrometry from whole liver and isolated hepatocytes. Serum and hepatocytic supernatant of IL ‐6, TNF ‐α and TGF ‐β levels were measured using ELISA . Cell signalling proteins were analysed using immunoblots. Results Regenerating liver after partial hepatectomy showed a significant increase of hepatic glucosylceramide in GBA 2‐deficient mice compared to controls. Accumulation of glucosylceramide was associated with a delay in liver regeneration and reduced serum levels of IL ‐6 and TNF ‐α. Furthermore, reduced IL ‐6 led to decreased expression of the phosphorylated form of the signal transducer and activator of transcription 3 ( P ‐ STAT 3). Conclusions We conclude that increased glucosylceramide affects cytokine‐ and growth factor‐mediated signalling pathways during liver regeneration. Thus, the repression of IL ‐6/ STAT 3 signalling pathway seems to be one of the mechanisms for the delay of liver regeneration in GBA 2‐deficient mice.
    Type of Medium: Online Resource
    ISSN: 1478-3223 , 1478-3231
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2124684-1
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  • 7
    Online Resource
    Online Resource
    Wiley ; 2004
    In:  Journal of Morphology Vol. 261, No. 3 ( 2004-09), p. 286-311
    In: Journal of Morphology, Wiley, Vol. 261, No. 3 ( 2004-09), p. 286-311
    Type of Medium: Online Resource
    ISSN: 0362-2525 , 1097-4687
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2004
    detail.hit.zdb_id: 1479991-1
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  • 8
    In: Medical Physics, Wiley, Vol. 45, No. 12 ( 2018-12), p. 5420-5436
    Abstract: Indirect‐detection CMOS flat‐panel detectors ( FPD s) offer fine pixel pitch, fast readout, and low electronic noise in comparison to current a‐Si:H FPD s. This work investigates the extent to which these potential advantages affect imaging performance in mobile C‐arm fluoroscopy and cone‐beam CT ( CBCT ). Methods FPD s based on CMOS (Xineos 3030 HS , 0.151 mm pixel pitch) or a‐Si:H (PaxScan 3030X, 0.194 mm pixel pitch) sensors were outfitted on equivalent mobile C‐arms for fluoroscopy and CBCT . Technical assessment of 2D and 3D imaging performance included measurement of electronic noise, gain, lag, modulation transfer function ( MTF ), noise‐power spectrum ( NPS ), detective quantum efficiency ( DQE ), and noise‐equivalent quanta ( NEQ ) in fluoroscopy (with entrance air kerma ranging 5–800  nG y per frame) and cone‐beam CT (with weighted CT dose index, CTDI w , ranging 0.08–1  mG y). Image quality was evaluated by clinicians in vascular, orthopaedic, and neurological surgery in realistic interventional scenarios with cadaver subjects emulating a variety of 2D and 3D imaging tasks. Results The CMOS FPD exhibited ~2–3× lower electronic noise and ~7× lower image lag than the a‐Si:H FPD . The 2D (projection) DQE was superior for CMOS at ≤50  nG y per frame, especially at high spatial frequencies (~2% improvement at 0.5 mm −1 and ≥50% improvement at 2.3 mm −1 ) and was somewhat inferior at moderate‐high doses (up to 18% lower DQE for CMOS at 0.5 mm −1 ). For smooth CBCT reconstructions (low‐frequency imaging tasks), CMOS exhibited ~10%–20% higher NEQ (at 0.1–0.5 mm −1 ) at the lowest dose levels ( CTDI w ≤0.1  mG y), while the a‐Si:H system yielded slightly (~5%) improved NEQ (at 0.1–0.5 lp/mm) at higher dose levels ( CTDI w ≥0.6  mG y). For sharp CBCT reconstructions (high‐frequency imaging tasks), NEQ was ~32% higher above 1 mm −1 for the CMOS system at mid‐high‐dose levels and ≥75% higher at the lowest dose levels ( CTDI w ≤0.1 mG y). Observer assessment of 2D and 3D cadaver images corroborated the objective metrics with respect to a variety of pertinent interventional imaging tasks. Conclusion Measurements of image noise, spatial resolution, DQE , and NEQ indicate improved low‐dose performance for the CMOS ‐based system, particularly at lower doses and higher spatial frequencies. Assessment in realistic imaging scenarios confirmed improved visibility of fine details in low‐dose fluoroscopy and CBCT . The results quantitate the extent to which CMOS detectors improve mobile C‐arm imaging performance, especially in 2D and 3D imaging scenarios involving high‐resolution tasks and low‐dose conditions.
    Type of Medium: Online Resource
    ISSN: 0094-2405 , 2473-4209
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1466421-5
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  • 9
    In: Medical Physics, Wiley, Vol. 42, No. 5 ( 2015-05), p. 2699-2708
    Abstract: To accelerate model‐based iterative reconstruction (IR) methods for C‐arm cone‐beam CT (CBCT), thereby combining the benefits of improved image quality and/or reduced radiation dose with reconstruction times on the order of minutes rather than hours. Methods: The ordered‐subsets, separable quadratic surrogates (OS‐SQS) algorithm for solving the penalized‐likelihood (PL) objective was modified to include Nesterov's method, which utilizes “momentum” from image updates of previous iterations to better inform the current iteration and provide significantly faster convergence. Reconstruction performance of an anthropomorphic head phantom was assessed on a benchtop CBCT system, followed by CBCT on a mobile C‐arm, which provided typical levels of incomplete data, including lateral truncation. Additionally, a cadaveric torso that presented realistic soft‐tissue and bony anatomy was imaged on the C‐arm, and different projectors were assessed for reconstruction speed. Results: Nesterov's method provided equivalent image quality to OS‐SQS while reducing the reconstruction time by an order of magnitude (10.0 ×) by reducing the number of iterations required for convergence. The faster projectors were shown to produce similar levels of convergence as more accurate projectors and reduced the reconstruction time by another 5.3 ×. Despite the slower convergence of IR with truncated C‐arm CBCT, comparison of PL reconstruction methods implemented on graphics processing units showed that reconstruction time was reduced from 106 min for the conventional OS‐SQS method to as little as 2.0 min with Nesterov's method for a volumetric reconstruction of the head. In body imaging, reconstruction of the larger cadaveric torso was reduced from 159 min down to 3.3 min with Nesterov's method. Conclusions: The acceleration achieved through Nesterov's method combined with ordered subsets reduced IR times down to a few minutes. This improved compatibility with clinical workflow better enables broader adoption of IR in CBCT‐guided procedures, with corresponding benefits in overcoming conventional limits of image quality at lower dose.
    Type of Medium: Online Resource
    ISSN: 0094-2405 , 2473-4209
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1466421-5
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  • 10
    In: Quarterly Journal of the Royal Meteorological Society, Wiley, Vol. 137, No. S1 ( 2011-01), p. 3-30
    Abstract: Within the framework of the international field campaign COPS (Convective and Orographically‐induced Precipitation Study), a large suite of state‐of‐the‐art meteorological instrumentation was operated, partially combined for the first time. This includes networks of in situ and remote‐sensing systems such as the Global Positioning System as well as a synergy of multi‐wavelength passive and active remote‐sensing instruments such as advanced radar and lidar systems. The COPS field phase was performed from 01 June to 31 August 2007 in a low‐mountain area in southwestern Germany/eastern France covering the Vosges mountains, the Rhine valley and the Black Forest mountains. The collected data set covers the entire evolution of convective precipitation events in complex terrain from their initiation, to their development and mature phase until their decay. Eighteen Intensive Observation Periods with 37 operation days and eight additional Special Observation Periods were performed, providing a comprehensive data set covering different forcing conditions. In this article, an overview of the COPS scientific strategy, the field phase, and its first accomplishments is given. Highlights of the campaign are illustrated with several measurement examples. It is demonstrated that COPS research provides new insight into key processes leading to convection initiation and to the modification of precipitation by orography, in the improvement of quantitative precipitation forecasting by the assimilation of new observations, and in the performance of ensembles of convection‐permitting models in complex terrain. Copyright © 2010 Royal Meteorological Society
    Type of Medium: Online Resource
    ISSN: 0035-9009 , 1477-870X
    URL: Issue
    RVK:
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2011
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    detail.hit.zdb_id: 2089168-4
    SSG: 14
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