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  • 1
    In: People and Nature, Wiley, Vol. 5, No. 4 ( 2023-08), p. 1094-1109
    Abstract: Read the free Plain Language Summary for this article on the Journal blog.
    Type of Medium: Online Resource
    ISSN: 2575-8314 , 2575-8314
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 3005781-4
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  • 2
    In: Molecular Ecology, Wiley, Vol. 31, No. 1 ( 2022-01), p. 104-118
    Abstract: Interpreting the formation of genomic variation landscape, especially genomic regions with elevated differentiation (i.e. islands), is fundamental to a better understanding of the genomic consequences of adaptation and speciation. Edaphic islands provide excellent systems for understanding the interplay of gene flow and selection in driving population divergence and speciation. However, discerning the relative contribution of these factors that modify patterns of genomic variation remains difficult. We analysed 132 genomes from five recently divergent species in Primulina genus, with four species distributed in Karst limestone habitats and the fifth one growing in Danxia habitats. We demonstrated that both gene flow and linked selection have contributed to genome‐wide variation landscape, where genomic regions with elevated differentiation (i.e., islands) were largely derived by divergent sorting of ancient polymorphism. Specifically, we identified several lineage‐specific genomic islands that might have facilitated adaptation of P. suichuanensis to Danxia habitats. Our study is amongst the first cases disentangling evolutionary processes that shape genomic variation of plant specialists, and demonstrates the important role of ancient polymorphism in the formation of genomic islands that potentially mediate adaptation and speciation of endemic plants in special soil habitats.
    Type of Medium: Online Resource
    ISSN: 0962-1083 , 1365-294X
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2020749-9
    detail.hit.zdb_id: 1126687-9
    SSG: 12
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  • 3
    In: Insect Science, Wiley, Vol. 19, No. 3 ( 2012-06), p. 419-425
    Type of Medium: Online Resource
    ISSN: 1672-9609
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2179775-4
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Wiley ; 2016
    In:  Insect Science Vol. 23, No. 2 ( 2016-04), p. 245-252
    In: Insect Science, Wiley, Vol. 23, No. 2 ( 2016-04), p. 245-252
    Abstract: Plant volatiles have been demonstrated to play an important role in regulating the behavior of Cotesia plutellae , a major larval parasitoid of the diamondback moth (DBM), Plutella xylostella , but little is currently known about the function of each volatile and their mixtures. We selected 13 volatiles of the DBM host plant, a cruciferous vegetable, to study the electroantennogram (EAG) and behavioral responses of C. plutellae . EAG responses to each of the compounds generally increased with concentration. Strong EAG responses were to 100 μL/mL of trans ‐2‐hexenal, benzaldehyde, nonanal and cis ‐3‐hexenol, and 10 μL/mL of trans ‐2‐hexenal and benzaldehyde with the strongest response provoked by trans ‐2‐hexenal at 100 μL/mL. In the Y‐tube olfactometer, C. plutellae , was significantly attracted by 1 μL/mL of trans ‐2‐hexenal and benzaldehyde. β‐caryophyllene, cis ‐3‐hexenol or trans ‐2‐hexenal significantly attracted C. plutellae at 10 μL/mL, while nonanal, benzyl alcohol, cis ‐3‐hexenol or benzyl cyanide at 100 μL/mL significantly attracted C. plutellae . Trans ‐2‐hexenal significantly repelled C. plutellae at 100 μL/mL. EAG of C. plutellae showed strong responses to all mixtures made of five various compounds with mixtures 3 ( trans ‐2‐hexenal, benzaldehyde, nonanal, cis ‐3‐hexenol, benzyl cyanide, farnesene, eucalyptol) and 4 ( trans ‐2‐hexenal, benzaldehyde, benzyl alcohol, ( R )‐(+)‐limonene, β‐ionone, farnesene, eucalyptol) significantly attracting C. plutellae . These findings demonstrate that the behavior of C. plutellae can be affected either by individual compounds or mixtures of plant volatiles, suggesting a potential of using plant volatiles to improve the efficiency of this parasitoid for biocontrol of P. xylostella .
    Type of Medium: Online Resource
    ISSN: 1672-9609 , 1744-7917
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2179775-4
    SSG: 12
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  • 5
    In: Archives of Insect Biochemistry and Physiology, Wiley, Vol. 102, No. 2 ( 2019-10)
    Abstract: DNA methylation exerts extensive impacts on gene expression of various living organisms exposed to environmental variation. However, little is known whether DNA methylation is involved in the host transfer of diamondback moth, Plutella xylostella (L.), a worldwide destructive pest of crucifers. In this study, we found that P. xylostella genome exhibited a relatively low level of DNA methylation on the basis of the CpG O/E prediction and experimental validation. A significant positive linear correlation was observed between the stage‐specific expressions of PxDNMT1 and DNA methylation levels (5mC content). Particularly, high levels of DNA methylation and gene expression of PxDNMT1 were observed in eggs and mature females of P. xylostella. After host transfer of P. xylostella from Raphanus sativus to Arabidopsis thaliana , we identified some potential genomic loci that might have changed methylation levels. Using the method of fluorescence‐labeled methylation‐sensitive amplified polymorphism (F‐MSAP), we also found the corresponding genes primarily involved in neural system and signaling. The expressions of six candidate genes were verified by qRT‐PCR. One of the genes, Px009600 , might be regulated by a DNA methylation‐mediated mechanism in response to host transfer. Our study provides evidence for a functional system of DNA methylation in P. xylostella and its possible role in adaptation during host transfer. Further studies should examine methylation as responsive factors to different host plants and environmental cues in insect pests.
    Type of Medium: Online Resource
    ISSN: 0739-4462 , 1520-6327
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1496071-0
    SSG: 12
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  • 6
    In: Pest Management Science, Wiley, Vol. 78, No. 7 ( 2022-07), p. 2838-2850
    Abstract: The tea green leafhopper, Empoasca onukii Matsuda, is the most destructive insect pest of tea plantations in East Asia. Despite its economic importance and previous studies on this species, it remains unclear as to how this small‐sized pest can have such wide range. RESULTS By sequencing three mitochondrial genes and 17 microsatellite loci, we revealed the regional structure and demographic expansion of 59 E. onukii populations in China. Bayesian analysis of population genetic structure (BAPS) on microsatellites identified four genetic groups with spatial discontinuities, while analysis on mitochondrial genes inferred five nested and differentiated clusters. Both the Mantel test and the generalized linear model indicated a significant pattern of isolation by geographic distance in E. onukii populations. Based on the approximate Bayesian computation approach, E. onukii was found to have originated from southwestern China and expanded northward and eastward. While MIGRATE‐N and Bayesian stochastic search variable selection (BSSVS) procedure in BEAST confirmed the possible eastward and northward dispersal from Yunnan, they also detected more gene flow from the derived populations in central and southeastern China. CONCLUSION Our results suggest that the current distribution and structure of E. onukii is complicatedly influenced by human activities of cultivation, wide dissemination of tea in ancient China as well as recent transportation of tea seedlings for establishing new tea plantations. Insights into genetic differentiation and demographic expansion patterns from this study provide an important basis for the development of area‐wide management of the E. onukii populations. © 2022 Society of Chemical Industry.
    Type of Medium: Online Resource
    ISSN: 1526-498X , 1526-4998
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2003455-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Wiley ; 1997
    In:  Ecology Vol. 78, No. 3 ( 1997-04), p. 666-
    In: Ecology, Wiley, Vol. 78, No. 3 ( 1997-04), p. 666-
    Type of Medium: Online Resource
    ISSN: 0012-9658
    RVK:
    Language: Unknown
    Publisher: Wiley
    Publication Date: 1997
    detail.hit.zdb_id: 1797-8
    detail.hit.zdb_id: 2010140-5
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Wiley ; 2007
    In:  The Journal of Wildlife Management Vol. 71, No. 1 ( 2007-02), p. 258-265
    In: The Journal of Wildlife Management, Wiley, Vol. 71, No. 1 ( 2007-02), p. 258-265
    Abstract: Abstract: We examined the relationship between the production of sites with feces (i.e., latrines) and river otter ( Lontra canadensis ) abundance to determine whether scat surveys were adequate for monitoring relative population size for species leaving activity signs in a clumped distribution on the landscape. We conducted winter riparian transects to simultaneously monitor otter abundance via snow tracks and latrine sites along the rivers of Kouchibouguac National Park and surrounding area in New Brunswick, Canada. Our data showed that latrine abundance poorly reflected otter abundance for given stretches of rivers because the relationship was nonlinear and reached a plateau. The number of latrine sites was not related to the time period since last snowfall, which indicated that otters repetitively defecated at the same sites. Individual otters and groups did not produce activity signs over larger distances as a function of time, which indicated that they tend to stay in their home ranges in winter. We discuss why scat survey protocols based on determining presence—absence of a species at predetermined search sites may poorly reflect population size, as well as population fluctuations in time. Caution is advised when interpreting data from such surveys for species for which feces or other activity signs surveyed play a role in intraspecific communication and tend to be in a clumped distribution on the landscape.
    Type of Medium: Online Resource
    ISSN: 0022-541X , 1937-2817
    Language: English
    Publisher: Wiley
    Publication Date: 2007
    detail.hit.zdb_id: 2066663-9
    SSG: 12
    SSG: 23
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  • 9
    In: Ecology and Evolution, Wiley, Vol. 5, No. 24 ( 2015-12), p. 5880-5891
    Abstract: Movement of individuals through events, such as storms or crop transportation, may affect survival and distribution of insect pests, as well as population genetic structure at a regional scale. Understanding what factors contribute to gene flow in pest populations remains very important for sustainable pest management. The diamondback moth ( Plutella xylostella ) is an insect pest well known for its capacity of moving over short to long distances. Here, we used newly isolated microsatellite markers to analyze the genetic structure of nine populations across the Taiwan Strait of China (Taiwan and Fujian). A total of 12,152 simple sequence repeats ( SSR s) were initially identified from the P. xylostella transcriptome (~94 Mb), with an average of 129 SSR s per Mb. Nine SSR s were validated to be polymorphic markers, and eight were used for this population genetic study. Our results showed that the P. xylostella populations could be divided into distinct two clusters, which is likely due to the year‐round airflows in this region. A pattern of isolation by distance among the local populations within Fujian was found, and may be related to vegetable transportation. Considering the complexity of the P. xylostella population genetic structure from local and regional to global levels, we propose that developing ecologically sound strategies for managing this pest will require knowledge of the link between behavioral and population ecology and its genetic structure.
    Type of Medium: Online Resource
    ISSN: 2045-7758 , 2045-7758
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2635675-2
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  • 10
    In: Starch - Stärke, Wiley, Vol. 70, No. 1-2 ( 2018-01)
    Abstract: A strain named Geobacillus thermoglucosidans CHB1 was isolated from stockpiled hen dung compost. The cyclodextrin glycosyltransferase (CGTase) from the fermentation broth of this microorganism was purified 350.50‐fold at a yield of 9.33% through ammonium sulfate precipitation, DEAE‐Sepharose Fast Flow chromatography, Sephadex G‐100 chromatography, and preparative electrophoresis. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) revealed that the molecular mass of the purified enzyme was approximately 70 kDa. The CGTase maintained more than 50% cyclization activity between 50 and 80°C and optimal activity at 65–70°C and remained stable at 50°C. Its optimal pH was 5.5, and its activity showed stability between pH 5.5 and 9.5. The activity of the enzyme was significantly enhanced by LiCl, NiCl 2 , and MgSO 4 but inhibited by AlCl 3 , CoCl 2 , FeCl 3 , HgCl 2 , BaCl 2 , SDS, and ZnSO 4 . The K m and V max of the reaction of this enzyme with soluble starch as the substrate were 12.5 mg/mL and 23.7 μmol/min, respectively. This enzyme produced α‐cyclodextrin (CD), β‐CD, and γ‐CD at a ratio of 0.57:1:0.21 from soluble starch as the substrate, and the conversion rate reached 60.3% from 3% soluble starch within 21 h. This CGTase also produced CDs from maltodextrin and potato starch as the substrate. To the best of our knowledge, this manuscript constitutes the first report of a CGTase purified from G. thermoglucosidans , and the results show that this enzyme might have potential for use in industrial production processes requiring stable thermal conditions, but further work is required to optimize its affinity and activation conditions.
    Type of Medium: Online Resource
    ISSN: 0038-9056 , 1521-379X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1481133-9
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