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  • 1
    In: The Clinical Respiratory Journal, Wiley, Vol. 15, No. 10 ( 2021-10), p. 1081-1087
    Abstract: Dyspnoea‐12 scale is a validated assessment tool, capturing the perception of dyspnoea and its physical and affective effects in individuals with chronic obstructive pulmonary disease (COPD). A validated version for the Chinese‐speaking population has been unavailable. Objective To develop a Chinese version of D‐12 (D‐12‐C) scale and evaluate its validity and reliability. Methods D‐12 was translated from English to traditional Chinese in collaboration with a physician and a linguist. Back translation was adopted to ensure accuracy of the translation. A total of 155 COPD patients were recruited to test the reliability and validity of the D‐12‐C scale. Internal reliability and test‐retest reliability were measured with Cronbach's alpha coefficient and intra‐class correlation coefficient, respectively. Construct validity was assessed through exploratory factor analysis (EFA). Concurrent validity was assessed by the correlation of D‐12‐C total score and sub‐scores and the Chinese version of Saint George's Respiratory Questionnaire (SGRQ), 36‐Item Short Form Health Survey (SF‐36), COPD Assessment Test (CAT) and Hospital Anxiety and Depression Scale (HADS) total score and sub‐scores. Results The two‐factor structure of D‐12‐C was confirmed by EFA. D‐12‐C and its sub‐scores demonstrated high level of internal reliability (Cronbach's alpha = 0.88) and moderate level of test‐retest reliability. D‐12‐C total score, physical and affective sub‐scores were significantly correlated to SGRQ total score ( r s  = 0.59, p   〈  0.001) and activity sub‐score ( r s  = 0.38, p  = 0.006), SF‐36 mental health sub‐score ( r s  = −0.36, p   〈  0.001), CAT ( r s  = 0.56, p   〈  0.001), HADS anxiety ( r s  = 0.51, p   〈  0.001) and depression sub‐scores ( r s  = 0.44, p   〈  0.001). Conclusion D‐12‐C scale was developed, which demonstrated satisfactory reliability and validity in measuring dyspnoea among COPD patients.
    Type of Medium: Online Resource
    ISSN: 1752-6981 , 1752-699X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2442214-9
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  • 2
    In: The Journal of Pathology, Wiley, Vol. 251, No. 1 ( 2020-05), p. 74-86
    Abstract: Serrated polyps are a clinically and molecularly heterogeneous group of lesions that can contribute to the development of colorectal cancers (CRCs). However, the molecular mechanism underlying the development of serrated lesions is still not well understood. Here, we combined multiple approaches to analyze the genetic alterations in 86 colorectal adenomas (including 35 sessile serrated lesions, 15 traditional adenomas, and 36 conventional adenomatous polyps). We also investigated the in vitro and in vivo oncogenic properties of a novel variant of the NCOA4–RET fusion gene. Molecular profiling revealed that sessile serrated lesions and traditional serrated adenomas have distinct clinicopathological and molecular features. Moreover, we identified receptor tyrosine kinase translocations exclusively in sessile serrated lesions (17%), and the observation was validated in a separate cohort of 34 sessile serrated lesions (15%). The kinase fusions as well as the BRAF and KRAS mutations were mutually exclusive to each other. Ectopic expression of a novel variant of the NCOA4–RET fusion gene promoted cell proliferation in vitro and in vivo , and the proliferation was significantly suppressed by RET kinase inhibitors. All of these underscored the importance of mitogen‐activated protein kinase (MAPK) pathway activation in the serrated pathway of colorectal tumorigenesis. In addition, we demonstrated that the kinase fusion may occur early in the precursor lesion and subsequent loss of TP53 may drives the transformation to carcinoma during serrated tumorigenesis. In conclusion, we identified kinase fusions as a significant alternative driver of the serrated pathway in colorectal cancer development, and detecting their presence may serve as a biomarker for the diagnosis of sessile serrated lesions. © 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0022-3417 , 1096-9896
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1475280-3
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