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  • Wiley  (35)
  • 1
    In: ChemBioChem, Wiley, Vol. 24, No. 4 ( 2023-02-14)
    Abstract: As an emerging therapeutic strategy, proteolysis‐targeting chimeras (PROTACs) have been proven to be superior to traditional drugs in many aspects. However, due to their unique mechanism of action, existing methods for evaluating the degradation still have many limitations, which seriously restricts the development of PROTACs. In this methodological study, using direct stochastic optical reconstruction microscopy (dSTORM)‐based single‐cell protein quantitative analysis, we systematically investigated the dynamic degradation characteristics of FLT3 protein during PROTACs treatment. We found that the distribution of FLT3 varies between FLT3‐ITD mutation and FLT3‐WT cells. PROTACs had an obvious time‐course effect on protein degradation and present two distinct phases; this provided a basis for deciding when to evaluate protein degradation. High concentrations of PROTACs were more effective than long‐time administration because a higher D max was achieved. Two‐color dSTORM‐based colocalization analysis efficiently detected the proportion of ternary complexes, making it very useful in screening PROTACs. Taken together, our findings show that the dSTORM method is an ideal tool for evaluating PROTACs and will accelerate the development of new PROTACs.
    Type of Medium: Online Resource
    ISSN: 1439-4227 , 1439-7633
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020469-3
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Wiley ; 2019
    In:  Optimal Control Applications and Methods Vol. 40, No. 4 ( 2019-07), p. 659-675
    In: Optimal Control Applications and Methods, Wiley, Vol. 40, No. 4 ( 2019-07), p. 659-675
    Abstract: Generalized predictive control (GPC) has been applied in systems to improve the control performance during the last decades. The designing of predictive model is the essential and vital problem in GPC application. Using characteristic model (CM) as predictive model in GPC can simplify the modeling procedure and improve computing efficiency of GPC. The CM is a kind of simple and easy‐to‐use dynamic model that is equivalent with precise model. Moreover, the creation of CM requires less system information than the practical models. The GPC with CM as predictive model named characteristic model–based generalized predictive control (CMGPC) is proposed in this paper, and it is used in the trajectory tracking control for parafoil and payload system. Simulations and analysis prove that CMGPC is equivalent to traditional GPC and CMGPC is a more efficient way for trajectory tracking of parafoil and payload system compared with conventional proportional integral derivative (PID) method.
    Type of Medium: Online Resource
    ISSN: 0143-2087 , 1099-1514
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2000658-5
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  • 3
    In: physica status solidi (b), Wiley, Vol. 259, No. 4 ( 2022-04)
    Abstract: Herein, low‐temperature and temperature‐dependent photoluminescence (PL) measurements are carried out on highly tensile‐strained Ge nanostructures embedded in GaSb matrix, and the effects of Ge deposition thickness are clarified. The direct‐gap transition‐related PL feature is successfully identified in the tensile‐strained Ge nanostructures. While typical PL thermal quenching is observed for the tensile‐strained Ge‐ and GaSb‐related transitions in the samples with a Ge deposition being thinner than the critical thickness, a negative thermal quenching shows up for the GaSb interband transition in the samples with Ge deposition surpassing the critical thickness at which high‐density nanoparticles form to relax the strain. A phenomenological thermal‐injection model is established of electrons from the tensile‐strained Ge layer to the GaSb matrix, the thermal quenching is accounted for, and a ladder‐like function of the strain‐relaxed Ge is clarified to favor the electron activation. The understanding of the effects of deposition thickness is helpful for the high‐performance Ge‐based light source for optoelectronic integration.
    Type of Medium: Online Resource
    ISSN: 0370-1972 , 1521-3951
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 208851-4
    detail.hit.zdb_id: 1481096-7
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  • 4
    In: Advanced Materials, Wiley, Vol. 35, No. 6 ( 2023-02)
    Abstract: Flexible microelectronics capable of straightforward implantation, remotely controlled navigation, and stable long‐term recording hold great promise in diverse medical applications, particularly in deciphering complex functions of neural circuits in the brain. Existing flexible electronics, however, are often limited in bending and buckling during implantation, and unable to access a large brain region. Here, an injectable class of electronics with stable recording, omnidirectional steering, and precise navigating capabilities based on magnetic actuation is presented. After simple transcriptional injection, the rigid coatings are biodegraded quickly and the bundles of magnetic‐nanoparticles‐coated microelectrodes become separated, ultra‐flexible, and magnetic actuated for further minimally invasive three‐dimensional interpenetration in the brain. As proof of concept, this paradigm‐shifting approach is demonstrated for selective and multiplexed neural activities recording across distant regions in the deep rodent brains. Coupling with optogenetic neural stimulation, the unique capabilities of this platform in electrophysiological readouts of projection dynamics in vivo are also demonstrated. The ability of these miniaturized, remotely controllable, and biocompatible ferromagnetic flexible electronics to afford minimally invasive manipulations in the soft tissues of the mammalian brain foreshadows applications in other organ systems, with great potential for broad utility in biomedical science and engineering.
    Type of Medium: Online Resource
    ISSN: 0935-9648 , 1521-4095
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1474949-X
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  • 5
    In: Advanced Healthcare Materials, Wiley
    Abstract: Since the microgap between implant and surrounding connective tissue creates the pass for pathogen invasion, sustained pathological stimuli can accelerate macrophage‐mediated inflammation, therefore affecting peri‐implant tissue regeneration and aggravate peri‐implantitis. As the transmucosal component of implant, the abutment therefore needs to be biofunctionalized to repair the gingival barrier. Here, a mussel‐bioinspired implant abutment coating containing tannic acid (TA), cerium and minocycline (TA‐Ce‐Mino) is reported. TA provides pyrogallol and catechol groups to promote cell adherence. Besides, Ce 3+ /Ce 4+  conversion exhibits enzyme‐mimetic activity to remove reactive oxygen species while generating O 2 , therefore promoting anti‐inflammatory M2 macrophage polarization to help create a regenerative environment. Minocycline is involved on the TA surface to create local drug storage for responsive antibiosis. Moreover, the underlying therapeutic mechanism is revealed whereby the coating exhibits exogenous antioxidation from the inherent properties of Ce and TA and endogenous antioxidation through mitochondrial homeostasis maintenance and antioxidases promotion. In addition, it stimulates integrin to activate PI3K/Akt and RhoA/ROCK pathways to enhance VEGF‐mediated angiogenesis and tissue regeneration. Combining the antibiosis and multidimensional orchestration, TA‐Ce‐Mino repairs soft tissue barriers and effector cell differentiation, thereby isolating the immune microenvironment from pathogen invasion. Consequently, this study provides critical insight into the design and biological mechanism of abutment surface modification to prevent peri‐implantitis.
    Type of Medium: Online Resource
    ISSN: 2192-2640 , 2192-2659
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2645585-7
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  • 6
    In: Journal of Ultrasound in Medicine, Wiley, Vol. 37, No. 6 ( 2018-06), p. 1389-1395
    Abstract: Imaging assessment of the female urethra is critical for diagnosis and treatment of urinary incontinence. High‐frequency 3‐dimensional (3D) transvaginal ultrasonography (TVUS) is a novel technique for evaluating the female urethra. The aim of this study was to test the repeatability of 3D TVUS between examiners without prior experience with TVUS. Methods Fifty women underwent 3D TVUS. Two examiners without prior experience analyzed the urethral volumes and measured the urethral parameters. Two‐dimensional (2D) parameters included urethral sphincter length and urethral sphincter thickness; 3D parameters included urethral sphincter volume, midurethral complex volume, and inner core volume. One of the examiners repeated the evaluations 1 month later. Subsequently, the measurements were compared. Results The mean age of the patients ± SD was 34.1 ± 8.1 (range, 23–55) years; the mean height, weight, and body mass index were 160.44 ± 5.12 (range, 150–173) cm, 61.80 ± 13.64 (range, 45–110) kg, and 23.98 ± 4.91 (range, 17.53–39.92) kg/m 2 , respectively. The results of our study showed excellent to good intraobserver repeatability (intraclass correlation coefficient [ICC], 0.75–0.87) for the evaluations of all parameters but urethral sphincter length (moderate ICC, 0.53), whereas they showed good to moderate interobserver repeatability (ICC, 0.44–0.77) for all parameters. The repeatability of 3D volumes (ICC, 0.59–0.87) tended to be better than that of 2D parameters (ICC, 0.44–0.76). Conclusions The intraobserver and interobserver repeatability of high‐frequency 3D TVUS measurements of the female urethra was excellent to moderate between examiners without previous experience. The repeatability of 3D measurements tended to be better than that of 2D parameters.
    Type of Medium: Online Resource
    ISSN: 0278-4297 , 1550-9613
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2067124-6
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  • 7
    In: Advanced Healthcare Materials, Wiley, Vol. 7, No. 11 ( 2018-06)
    Abstract: Small‐diameter ( 〈 6 mm) tissue–engineered blood vessels (TEBVs) have a low patency rate due to chronic inflammation mediated intimal hyperplasia. Functional coating with drug release is a promising solution, but preventing the released drug from being rushed away by blood flow remains a great challenge. A single‐walled carboxylic acid functionalized carbon nanotube (C‐SWCNT) is used to build an irregular mesh for TEBV coating. However, an interaction between the released drug and the cells is still insufficient due to the blood flow. Thus, an intracellular drug delivery system mediated by macrophage cellular uptake is designed. Resveratrol (RSV) modified CNT is used for macrophage uptake. M1 macrophage uptakes CNT‐RSV and then converts to the M2 phenotype upon intracellular RSV release. Prohealing M2 macrophage inhibits the chronic inflammation thus maintains the contractile phenotype of the vascular smooth muscle cell (VSMC), which reduces intimal hyperplasia. Additionally, RSV released from the mesh coating also directly protects the contractile VSMCs from being converted to a secretory phenotype. Through antishear stress coating and macrophage‐based intracellular drug delivery, CNT‐RSV TEBVs exhibit a long‐term anti‐intimal hyperplasia function. Animal transplantation studies show that the patency rate remains high until day 90 after grafting in rat carotid arteries.
    Type of Medium: Online Resource
    ISSN: 2192-2640 , 2192-2659
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2645585-7
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  • 8
    In: European Journal of Neurology, Wiley
    Abstract: Prodromal infections are associated with neuromyelitis optica spectrum disorder (NMOSD), but it remains unclear which type of infection has a causal association with NMOSD. We aimed to explore the causal associations between four herpesvirus infections (chickenpox, cold sores, mononucleosis and shingles) and NMOSD, as well as between other types of infections and NMOSD. Methods For data on infections, we used the genome‐wide association study (GWAS) summary statistics from the 23andMe cohort. For outcomes, we used the GWAS data of participants of European ancestry, including 215 NMOSD patients (132 anti‐aquaporin‐4 antibody [AQP4‐ab]‐positive patients and 83 AQP4‐ab‐negative patients) and 1244 normal controls. Single‐nucleotide polymorphism (SNP) identification and two‐sample Mendelian randomization (MR) analyses were then performed. Results In the 23andMe cohort, we identified one SNP for chickenpox (rs9266089 in HLA‐B gene), one SNP for cold scores (rs885950 in the POU5F1 gene), one SNP for mononucleosis (rs2596465 in the HCP5 gene), and three SNPs for shingles (rs2523591 in the HLA‐B gene; rs7047299 in the IFNA21 gene; rs9260809 in the MICD gene). The association between cold sores and AQP4‐ab‐positive NMOSD reached statistical significance (odds ratio [OR] 745.318; 95% confidence interval [CI] 22.176, 25,049.53 [ p   〈  0.001, Q   〈  0.001]). The association between shingles and AQP4‐ab‐positive NMOSD was also statistically significant (OR 21.073; 95% CI 4.271, 103.974 [ p   〈  0.001, Q   〈  0.001]). No significant association was observed between other infections and AQP4‐ab‐positive or AQP4‐ab‐negative NMOSD. Conclusion These findings suggest there are positive associations between cold sores and shingles and AQP4‐ab‐positive NMOSD, indicating there may be causal links between herpes simplex virus and varicella‐zoster virus infection and AQP4‐ab‐positive NMOSD.
    Type of Medium: Online Resource
    ISSN: 1351-5101 , 1468-1331
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020241-6
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  • 9
    In: New Phytologist, Wiley, Vol. 230, No. 5 ( 2021-06), p. 1940-1952
    Abstract: Pre‐harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. Here, we mapped the PHS resistance gene PHS‐3D from synthetic hexaploid wheat to a 2.4 Mb presence–absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropic Myb10‐D by integrated omics and functional analyses. Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii , and the germination percentages of wheat lines containing Myb10‐D was approximately 40% lower than that of the other lines. Transcriptome and metabolome profiling indicated that Myb10‐D affected the transcription of genes in both the flavonoid and abscisic acid (ABA) biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines. Myb10‐D activates 9‐ cis ‐epoxycarotenoid dioxygenase ( NCED ) by biding the secondary wall MYB‐responsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function of Myb10‐D confers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. The PAV harboring Myb10‐D associated with grain color and PHS will be useful for understanding and selecting white grained PHS resistant wheat cultivars.
    Type of Medium: Online Resource
    ISSN: 0028-646X , 1469-8137
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 208885-X
    detail.hit.zdb_id: 1472194-6
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  • 10
    In: Zeitschrift für anorganische und allgemeine Chemie, Wiley, Vol. 643, No. 14 ( 2017-08-03), p. 878-882
    Type of Medium: Online Resource
    ISSN: 0044-2313
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 201094-X
    detail.hit.zdb_id: 1481139-X
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