In:
The Plant Journal, Wiley, Vol. 118, No. 6 ( 2024-06), p. 2296-2317
Abstract:
Given that plants contain impurities in their leaves, DNA purification is required to construct NGS libraries with reduced genomic complexity using restriction enzymes, which poses a hinderance to rapid genotyping. We developed degenerate oligonucleotide primer MIG‐seq and its accompanying protocol, a method for constructing NGS libraries with varying degrees of complexity reduction from unpurified plant DNA using PCR, and demonstrated the feasibility of expeditious genetic analysis across various crop species.
Type of Medium:
Online Resource
ISSN:
0960-7412
,
1365-313X
Language:
English
Publisher:
Wiley
Publication Date:
2024
detail.hit.zdb_id:
2020961-7
SSG:
12
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