In:
International Journal of Cancer, Wiley, Vol. 145, No. 1 ( 2019-07), p. 232-241
Abstract:
What's new? Early metastatic spread to sentinel lymph nodes provides important staging information for melanoma patients. In addition, adjuvant therapies are increasingly explored in node‐positive patients, making the availability of companion diagnostics necessary. Thus, here the authors established a protocol for the analysis of single disseminated cancer cells (DCCs) from lymph nodes applying a commercially available epitope‐independent microfluidic enrichment method. They show that cell suspension from lymph nodes can be processed and that microfluidics‐enriched cells are viable and amenable for subsequent molecular analysis. The presented workflow represents a novel tool for in‐depth characterization of lymph node‐derived cancer cells, including early metastasis precursors.
Type of Medium:
Online Resource
ISSN:
0020-7136
,
1097-0215
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
218257-9
detail.hit.zdb_id:
1474822-8
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