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  • 1
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    Identification of Four Gossypium sturtianum Monosomic Alien Addition Derivatives from a Backcrossing Program with G. hirsutum
    Wiley ; 1991
    In:  Crop Science Vol. 31, No. 2 ( 1991-03), p. 337-341
    Details
    In: Crop Science, Wiley, Vol. 31, No. 2 ( 1991-03), p. 337-341
    Abstract: Alien chromosome addition lines provide means to distinguish effects of specific alien chromosomes, to detect homoeologies between chromosomes of different species, and to conduct chromosome‐specific introgression. Over the last few years, numerous monosomic addition (MA) stocks were derived from interspecific backcrosses of Gossypium sturtianum J.H. Willis (2 n = 2 x = 26, C 1 genome) with G. hirsutum L. [2 n = 4 x = 52, (AD), genome] as recurrent parent. Using 10 MA plants of varied pedigrees from this project, our objectives were to (i) identify and characterize different MA stocks, (ii) determine the phenol) pic effect of each addition chromosome, and (iii) estimate the frequency of (AD) 1 ‐C 1 recombination. We identified 4 distinct G. sturtianum MA types among the 10 analyzed, and have temporarily designated these as C 1 ‐A, C 1 ‐B, C 1 ‐C, and C 1 ‐D. The C 1 ‐A, C 1 ‐B, and C 1 ‐D MA stocks differed phenotypically from the recurrent parent, while the C 1 ‐C MA stock was phenotypically indistinguishable from the recurrent parent. None of the MA chromosomes was pollen transmissible, but all were ovule transmissible in vivo. Frequencies of ovule transmission from the MA chromosomes ranged 13.7% for C 1 ‐B to 77% for C 1 ‐A. Cytogenetic analyses of the parental stocks and their backcross progenies revealed low but nonetheless increased frequencies of abnormal meiotic chromosomal configurations, possibly due to previous intergenomic recombination, the meiotic disturbances, or both. Genetic data to substantiate recombination have not yet been obtained, but chiasmata were observed at a frequency of 1.7% per meiotic C 1 chromosome in the MA stocks, indicating that recombination with chromosomes of G. hirsutum is occurring, albeit infrequently. The establishment and characterization of these four MA stocks will facilitate development of dispersed repetitive genomic probes for the C 1 genome, analysis of interspecific C 1 ‐to‐(AD) 1 genomic introgression, and the derivation of additional C 1 MA stocks.
    Type of Medium: Online Resource
    ISSN: 0011-183X , 1435-0653
    URL: Article
    DOI: 10.2135/cropsci1991.0011183X003100020024x
    Language: English
    Publisher: Wiley
    Publication Date: 1991
    detail.hit.zdb_id: 1480918-7
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  • 2
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    A novel and efficient method for identifying cotton leafroll dwarf virus infection in upland cotton ( Gossypium hirsutum )
    Wiley ; 2023
    In:  Plant Breeding Vol. 142, No. 2 ( 2023-04), p. 238-246
    Details
    In: Plant Breeding, Wiley, Vol. 142, No. 2 ( 2023-04), p. 238-246
    Abstract: We report the development of a novel and more efficient, rapid, cost‐effective and simple technique than current PCR‐based identification methods for screening cotton ( Gossypium hirsutum ) plants for the presence of cotton leafroll dwarf virus (CLRDV). This protocol takes advantage of the PACE (PCR Allele Competitive Extension) system and uses PCR amplification of cDNA, coupled with sequence‐specific fluorescent probes to differentiate between infected and uninfected cotton plants. This procedure has the potential for application in detection of other RNA viruses in a variety of other crops, by using primers specific for the RNA‐dependent RNA polymerase (RdRP) gene and a widely conserved housekeeping gene in the host organism; in this case, the G. hirsutum polyubiquitin gene (GhUB).
    Type of Medium: Online Resource
    ISSN: 0179-9541 , 1439-0523
    URL: Issue
    URL: Article
    DOI: 10.1111/pbr.v142.2
    DOI: 10.1111/pbr.13081
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020488-7
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  • 3
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    Evolution of interspersed repetitive elements in Gossypium (Malvaceae)
    Wiley ; 1998
    In:  American Journal of Botany Vol. 85, No. 10 ( 1998-10), p. 1364-1368
    Details
    In: American Journal of Botany, Wiley, Vol. 85, No. 10 ( 1998-10), p. 1364-1368
    Abstract: Very little is known regarding how repetitive elements evolve in polyploid organisms. Here we address this subject by fluorescent in situ hybridization (FISH) of 20 interspersed repetitive elements to metaphase chromosomes of the cotton AD‐genome tetraploid Gossypium hirsutum and its putative A‐ and D‐genome diploid ancestors. These elements collectively represent an estimated 18% of the G. hirsutum genome, and constitute the majority of high‐copy interspersed repetitive elements in G. hirsutum . Seventeen of the elements yielded FISH signals on chromosomes of both G. hirsutum subgenomes, while three were A‐subgenome specific. Hybridization of eight selected elements, two of which were A‐subgenome specific, to the A 2 genome of G. arboreum yielded a signal distribution that was similar to that of the G. hirsutum A‐subgenome. However, when hybridized to the D 5 genome of G. raimondii , the putative diploid ancestor of the G. hirsutum D‐subgenome, none of the probes, including elements that strongly hybridized to both G. hirsutum subgenomes, yielded detectable signal. The results suggest that the majority, although not all, G. hirsutum interspersed repetitive elements have undergone intergenomic concerted evolution following polyploidization and that this has involved colonization of the D‐subgenome by A‐subgenome elements and/or replacement of D‐subgenome elements by elements of the A‐subgenome type.
    Type of Medium: Online Resource
    ISSN: 0002-9122 , 1537-2197
    URL: Article
    DOI: 10.2307/2446394
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 1998
    detail.hit.zdb_id: 2053581-8
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  • 4
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    A Long‐Read Transcriptome Assembly of Cotton ( Gossypium hirsutum L.) and Intraspecific Single Nucleotide Polymorphism Discovery
    Wiley ; 2015
    In:  The Plant Genome Vol. 8, No. 2 ( 2015-07)
    Details
    In: The Plant Genome, Wiley, Vol. 8, No. 2 ( 2015-07)
    Abstract: Upland cotton ( Gossypium hirsutum L.) has a narrow germplasm base, which constrains marker development and hampers intraspecific breeding. A pressing need exists for high‐throughput single nucleotide polymorphism (SNP) markers that can be readily applied to germplasm in breeding and breeding‐related research programs. Despite progress made in developing new sequencing technologies during the past decade, the cost of sequencing remains substantial when one is dealing with numerous samples and large genomes. Several strategies have been proposed to lower the cost of sequencing for multiple genotypes of large‐genome species like cotton, such as transcriptome sequencing and reduced‐representation DNA sequencing. This paper reports the development of a transcriptome assembly of the inbred line Texas Marker‐1 (TM‐1), a genetic standard for cotton, its usefulness as a reference for RNA sequencing (RNA‐seq)‐based SNP identification, and the availability of transcriptome sequences of four other cotton cultivars. An assembly of TM‐1 was made using Roche 454 transcriptome reads combined with an assembly of all available public expressed sequence tag (EST) sequences of TM‐1. The TM‐1 assembly consists of 72,450 contigs with a total of 70 million bp. Functional predictions of the transcripts were estimated by alignment to selected protein databases. Transcriptome sequences of the five lines, including TM‐1, were obtained using an Illumina Genome Analyzer‐II, and the short reads were mapped to the TM‐1 assembly to discover SNPs among the five lines. We identified 〉 14,000 unfiltered allelic SNPs, of which ∼3,700 SNPs were retained for assay development after applying several rigorous filters. This paper reports availability of the reference transcriptome assembly and shows its utility in developing intraspecific SNP markers in upland cotton.
    Type of Medium: Online Resource
    ISSN: 1940-3372 , 1940-3372
    URL: Article
    DOI: 10.3835/plantgenome2014.10.0068
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2440458-5
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  • 5
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    SNP‐Based MAS in Cotton under Depressed‐Recombination for Ren lon –Flanking Recombinants: Results and Inferences on Wide‐Cross Breeding Strategies
    Wiley ; 2016
    In:  Crop Science Vol. 56, No. 4 ( 2016-07), p. 1526-1539
    Details
    In: Crop Science, Wiley, Vol. 56, No. 4 ( 2016-07), p. 1526-1539
    Abstract: Strong resistance to reniform nematode ( Rotylenchulus reniformis ) was previously introgressed from the F‐genome diploid species Gossypium longicalyx (2 n = 2 x = 26) into Upland cotton ( G. hirsutum L., 2 n = 4 x = 52, 2[AD] 1 ), and attributed to the gene Ren lon . Two resistant elite lines were released, but their seedlings are differentially stunted in nematode‐infested fields. Here, we report on the development of linked SNPs and their use to disrupt “linkage drag” around Ren lon . Using advanced backcross‐inbred lines with previously identified proximal (PCO) or distal crossover (DCO) events near Ren lon , we chose 18 Ren lon –linked SNP markers for mapping across two large (880) BC 1 F 1 seed populations. Few recombinants occurred (7 of 1760). Using two of the closest Ren lon –linked SNPs to select from a BC 1 F 1 seed population (17,600), we identified 5 homeologous recombinants, but none separated the stunting and resistant phenotypes. We then compared homologous recombination rates using equally sized DCO × PCO INTERCROSS and BACKCROSS populations ( n = 88). Many more recombinants occurred among progeny of the INTERCROSS (22) than the BACKCROSS (1). This finding suggests that the best SNP‐based strategy for interspecific breeding with the F genome and other homeologous genomes may be to use large‐scale MAS to select nearby flanking homeologous recombinants in early generations, map them to identify the two closest PCO and DCO types, then intermate them and use MAS to identify homologous recombinants.
    Type of Medium: Online Resource
    ISSN: 0011-183X , 1435-0653
    URL: Article
    DOI: 10.2135/cropsci2015.07.0436
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1480918-7
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  • 6
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    Executive Vice‐president's Report, 1979
    Wiley ; 1980
    In:  Agronomy Journal Vol. 72, No. 1 ( 1980-01), p. 205-209
    Details
    In: Agronomy Journal, Wiley, Vol. 72, No. 1 ( 1980-01), p. 205-209
    Type of Medium: Online Resource
    ISSN: 0002-1962 , 1435-0645
    URL: Article
    DOI: 10.2134/agronj1980.00021962007200010047x
    Language: English
    Publisher: Wiley
    Publication Date: 1980
    detail.hit.zdb_id: 1471598-3
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  • 7
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    FORMATION OF 2N MEGAGAMETOPHYTES IN DIPLOID TUBER‐BEARING SOLANUMS
    Wiley ; 1986
    In:  American Journal of Botany Vol. 73, No. 9 ( 1986-09), p. 1351-1363
    Details
    In: American Journal of Botany, Wiley, Vol. 73, No. 9 ( 1986-09), p. 1351-1363
    Abstract: Development of megaspores and megagametophytes was analyzed for several diploid potato clones ( Solanum spp.) that exhibit either high (HI) or low (LO) seed set when crossed as female with the tetraploid cultivated potato S. tuberosum Group Tuberosa. The objectives were to determine the relationship between ploidy and diam of nuclei and nucleoli, and to determine the mechanism(s) and frequencies of 2 n megagametophyte formation. Sizes of nuclei and nucleoli were found to depend on ploidy. For HI clones, the distributions of sizes indicated that doubling occurred during meiosis, and that 30 to 50% of the megaspores and megagametophytes were 2 n rather than haploid. Omission of the second meiotic division led to formation of second division restitution (SDR) 2 n megagametophytes. Only one HI clone had abnormal meiosis I, in addition to omission of meiosis II in some meiocytes; this clone seemed to produce not only 1 n and 2 n , but also 4 n megagametophytes. The results indicated that high crossability of the HI clones as female with tetraploids largely was due to formation of SDR 2 n megagametophytes, a finding strongly supporting the hypothesis that sexual polyploidization is the driving force behind polyploidization of Solanums. The results contribute to increasing evidence that meiotic mutants and abnormalities play an important role in angiosperm evolution.
    Type of Medium: Online Resource
    ISSN: 0002-9122 , 1537-2197
    URL: Issue
    URL: Article
    DOI: 10.1002/ajb2.1986.73.issue-9
    DOI: 10.1002/j.1537-2197.1986.tb10876.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 1986
    detail.hit.zdb_id: 2053581-8
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  • 8
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    Genotype Dependent Interspecific Hybridization of Sorghum bicolor
    Wiley ; 2006
    In:  Crop Science Vol. 46, No. 6 ( 2006-11), p. 2617-2622
    Details
    In: Crop Science, Wiley, Vol. 46, No. 6 ( 2006-11), p. 2617-2622
    Abstract: Wild Australian Sorghum species are a tertiary gene pool to grain sorghum [ Sorghum bicolor (L.) Moench], and they are of interest to breeders because of their resistance to important insects and pathogens. However, strong reproductive barriers have prevented hybridization between S. bicolor and these wild species. The purpose of this study was to determine if the recessive iap allele (dominant allele Iap = inhibition of alien pollen ) would reduce or eliminate the pollen–pistil incompatibilities that prevent hybridization between S. bicolor and divergent Sorghum species. Cytoplasmic male‐sterile S. bicolor plants, homozygous for the iap allele, were pollinated with three divergent Sorghum species, S. angustum Blake, S. nitidum (Vahl) Pers., and S. macrospermum Garber. The pollen of these three wild species readily germinated and the pollen tubes grew to the base of the S. bicolor ovary within 2 h after pollination. Hybrid embryos were detected in the S. bicolor florets 13 to 20 d post‐pollination. Sorghum bicolor × S. angustum and S. bicolor × S. nitidum hybrids were obtained using embryo rescue followed by in vitro culture techniques and hybrids between S. bicolor and S. macrospermum were obtained by simply germinating the hybrid seed. These hybrids were confirmed by their morphological and cytological traits. These findings clearly demonstrate that the recessive iap allele circumvents pollen–pistil incompatibilities in the genus Sorghum and permits hybrids to be made between S. bicolor and species of the tertiary gene pool.
    Type of Medium: Online Resource
    ISSN: 0011-183X , 1435-0653
    URL: Article
    DOI: 10.2135/cropsci2005.09.0295
    Language: English
    Publisher: Wiley
    Publication Date: 2006
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  • 9
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    Linkage Mapping of Resistance to Reniform Nematode in Cotton following Introgression from Gossypium longicalyx (Hutch. & Lee)
    Wiley ; 2009
    In:  Crop Science Vol. 49, No. 4 ( 2009-07), p. 1151-1164
    Details
    In: Crop Science, Wiley, Vol. 49, No. 4 ( 2009-07), p. 1151-1164
    Abstract: Reniform nematodes ( Rotylenchulus reniformis Linford & Oliveira) decrease U.S. production of Upland cotton ( Gossypium hirsutum L., 2 n = 52, 2[AD] 1 ) by more than US$100 million yr −1 We report here on the mapping of a gene for extreme resistance that was introgressed from the African species G. longicalyx (Hutch. & Lee, 2 n = 2x = 26; 2F 1 ). The responsible allele, designated Ren lon , was localized to chromosome 11 by first screening A‐subgenome simple sequence repeat (SSR) marker loci for parental polymorphism and then for association with resistance. The three most strongly coupled SSRs and a G. longicalyx gene conferring green seed fuzz, designated Fzg lon , were screened against 984 resistant and susceptible individuals of multiple backcross generations. We used marker data and pedigrees to identify nonrecombinant heterozygous parents and thereby avoid bias from repeated sampling of a recombination event. We constructed linkage maps after progeny testing a small population (147) and after implementing three alternative approaches better suited to larger populations—marker‐assisted genotyping analysis, applying a cut‐off value as population‐wide genotyping criterion, and genotype‐selective sampling. The maps concordantly indicated the order to be Fzg lon – Ren lon –BNL3279_114–BNL1066_156–BNL836_215, with most Ren ‐proximal bilaterally flanking markers within 6 cM of each other. The results will clearly facilitate use of Ren lon in breeding, additional mapping, genomics, and prospective cloning.
    Type of Medium: Online Resource
    ISSN: 0011-183X , 1435-0653
    URL: Article
    DOI: 10.2135/cropsci2008.03.0129
    Language: English
    Publisher: Wiley
    Publication Date: 2009
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  • 10
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    Proposed Schemes for Mass‐Extraction of Doubled Haploids of Cotton
    Wiley ; 1988
    In:  Crop Science Vol. 28, No. 6 ( 1988-11), p. 885-890
    Details
    In: Crop Science, Wiley, Vol. 28, No. 6 ( 1988-11), p. 885-890
    Abstract: We propose (i) synthesis of hybrid‐eliminating, haploid‐inducing (HEHP) lines and populations homozygous for the Le 2 dav hybrid lethality allele and the Se allele for semigamous reproduction, (ii) cross pollination of HEHP plants with pollen of normal cotton ( Gossypium hirsutum and G. barbadense ) to form inviable true hybrids, but viable haploids, and (iii) bulk treatment of all seed or young viable seedlings with colchicine to recover doubled haploids en masse. Doubled paternal haploid plants and sectors will be phenotypically unique, as distinguished by fertility, size of plant parts, and expression of genetic marker(s). Inclusion of male sterility in segregating HEHP populations will facilitate manual or natural cross pollination, allowing large‐scale usage. To convert cytoplasms of HEHP materials, available semigamous lines with the desired cytoplasm can be crossed with a HEHP pollen parent to obtain paternal haploid progeny that can be colchicine‐doubled. The HEHP materials should facilitate all types of cytogenetic manipulations involving extraction of doubled (paternal) haploids. The basic concepts and methods proposed might be extrapolatable to other crops.
    Type of Medium: Online Resource
    ISSN: 0011-183X , 1435-0653
    URL: Article
    DOI: 10.2135/cropsci1988.0011183X002800060002x
    Language: English
    Publisher: Wiley
    Publication Date: 1988
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